Restriction map for
pF1KB7413
(Linear) (Six-base) MAPSORT of: KIBB7413.seq Check: 2441 from: 1 to: 1155
KIBB7413.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 662 1155
Size: 662 493
AccI GT'mk_AC
Cuts at: 0 237 1155
Size: 237 918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1023 1155
Size: 1023 132
AhdI GACnn_n'nnGTC
Cuts at: 0 798 1155
Size: 798 357
AlwNI CAG_nnn'CTG
Cuts at: 0 540 757 790 1063 1155
Size: 540 217 33 273 92
Fragments arranged by size:
540 273 217 92 33
ApaI G_GGCC'C
Cuts at: 0 945 1155
Size: 945 210
ApaLI G'TGCA_C
Cuts at: 0 551 1155
Size: 551 604
AvaI C'yCGr_G
Cuts at: 0 594 875 1155
Size: 594 281 280
Fragments arranged by size:
594 281 280
BanI G'GyrC_C
Cuts at: 0 507 696 1010 1155
Size: 507 189 314 145
Fragments arranged by size:
507 314 189 145
BanII G_rGCy'C
Cuts at: 0 710 885 945 1155
Size: 710 175 60 210
Fragments arranged by size:
710 210 175 60
BbeI G_GCGC'C
Cuts at: 0 700 1155
Size: 700 455
BbsI GAAGACnn'nnnn_
Cuts at: 0 458 1115 1155
Size: 458 657 40
Fragments arranged by size:
657 458 40
BbvCI CC'TCA_GC
Cuts at: 0 909 1155
Size: 909 246
BclI T'GATC_A
Cuts at: 0 467 1155
Size: 467 688
BglI GCCn_nnn'nGGC
Cuts at: 0 626 1155
Size: 626 529
BlpI GC'TnA_GC
Cuts at: 0 287 583 1155
Size: 287 296 572
Fragments arranged by size:
572 296 287
Bme1580I G_kGCm'C
Cuts at: 0 555 945 1013 1155
Size: 555 390 68 142
Fragments arranged by size:
555 390 142 68
BmrI ACTGGGnnnn_n'
Cuts at: 0 69 1155
Size: 69 1086
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 418 1155
Size: 418 737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 724 740 1155
Size: 505 219 16 415
Fragments arranged by size:
505 415 219 16
Bpu10I CC'TnA_GC
Cuts at: 0 512 909 1155
Size: 512 397 246
Fragments arranged by size:
512 397 246
BsaAI yAC'GTr
Cuts at: 0 498 1155
Size: 498 657
BsaHI Gr'CG_yC
Cuts at: 0 697 1155
Size: 697 458
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 330 1155
Size: 330 825
BseYI C'CCAG_C
Cuts at: 0 216 533 750 1006 1155
Size: 216 317 217 256 149
Fragments arranged by size:
317 256 217 216 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 331 1155
Size: 167 164 824
Fragments arranged by size:
824 167 164
BsiHKAI G_wGCw'C
Cuts at: 0 555 885 1155
Size: 555 330 270
Fragments arranged by size:
555 330 270
BsmI GAATG_Cn'
Cuts at: 0 141 1155
Size: 141 1014
Bsp1286I G_dGCh'C
Cuts at: 0 555 710 885 945 1013 1155
Size: 555 155 175 60 68 142
Fragments arranged by size:
555 175 155 142 68 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 662 1155
Size: 662 493
BsrGI T'GTAC_A
Cuts at: 0 363 1155
Size: 363 792
BssSI C'ACGA_G
Cuts at: 0 776 1155
Size: 776 379
BstXI CCAn_nnnn'nTGG
Cuts at: 0 658 1155
Size: 658 497
BstYI r'GATC_y
Cuts at: 0 102 383 1155
Size: 102 281 772
Fragments arranged by size:
772 281 102
BtgI C'CryG_G
Cuts at: 0 686 1155
Size: 686 469
EaeI y'GGCC_r
Cuts at: 0 127 1155
Size: 127 1028
EarI CTCTTCn'nnn_
Cuts at: 0 11 409 1142 1155
Size: 11 398 733 13
Fragments arranged by size:
733 398 13 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 875 1155
Size: 875 280
EcoICRI GAG'CTC
Cuts at: 0 883 1155
Size: 883 272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 724 740 1023 1155
Size: 505 219 16 283 132
Fragments arranged by size:
505 283 219 132 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1051 1075 1155
Size: 1051 24 80
Fragments arranged by size:
1051 80 24
EcoO109I rG'GnC_Cy
Cuts at: 0 320 744 787 1155
Size: 320 424 43 368
Fragments arranged by size:
424 368 320 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 972 1155
Size: 972 183
HaeII r_GCGC'y
Cuts at: 0 540 700 1155
Size: 540 160 455
Fragments arranged by size:
540 455 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 784 816 1155
Size: 784 32 339
Fragments arranged by size:
784 339 32
HindIII A'AGCT_T
Cuts at: 0 977 1155
Size: 977 178
KasI G'GCGC_C
Cuts at: 0 696 1155
Size: 696 459
MscI TGG'CCA
Cuts at: 0 129 1155
Size: 129 1026
MslI CAynn'nnrTG
Cuts at: 0 460 656 1155
Size: 460 196 499
Fragments arranged by size:
499 460 196
MspA1I CmG'CkG
Cuts at: 0 207 291 1060 1155
Size: 207 84 769 95
Fragments arranged by size:
769 207 95 84
NarI GG'CG_CC
Cuts at: 0 697 1155
Size: 697 458
PasI CC'CwG_GG
Cuts at: 0 433 1155
Size: 433 722
PflMI CCAn_nnn'nTGG
Cuts at: 0 757 1155
Size: 757 398
PfoI T'CCnGG_A
Cuts at: 0 740 1155
Size: 740 415
PmlI CAC'GTG
Cuts at: 0 498 1155
Size: 498 657
PpuMI rG'GwC_Cy
Cuts at: 0 744 1155
Size: 744 411
PspOMI G'GGCC_C
Cuts at: 0 941 1155
Size: 941 214
PvuII CAG'CTG
Cuts at: 0 291 1060 1155
Size: 291 769 95
Fragments arranged by size:
769 291 95
SacI G_AGCT'C
Cuts at: 0 885 1155
Size: 885 270
SapI GCTCTTCn'nnn_
Cuts at: 0 11 1142 1155
Size: 11 1131 13
Fragments arranged by size:
1131 13 11
SfcI C'TryA_G
Cuts at: 0 247 1155
Size: 247 908
SfoI GGC'GCC
Cuts at: 0 698 1155
Size: 698 457
SmaI CCC'GGG
Cuts at: 0 596 877 1155
Size: 596 281 278
Fragments arranged by size:
596 281 278
StyI C'CwwG_G
Cuts at: 0 130 1155
Size: 130 1025
TatI w'GTAC_w
Cuts at: 0 363 1155
Size: 363 792
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 131 163 1155
Size: 131 32 992
Fragments arranged by size:
992 131 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 262 1155
Size: 262 893
XmaI C'CCGG_G
Cuts at: 0 594 875 1155
Size: 594 281 280
Fragments arranged by size:
594 281 280
XmnI GAAnn'nnTTC
Cuts at: 0 119 1155
Size: 119 1036
Enzymes that do cut:
AarI AccI AcuI AhdI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BbvCI BclI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrGI BssSI
BstXI BstYI BtgI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HindIII KasI MscI
MslI MspA1I NarI PasI PflMI PfoI PmlI PpuMI
PspOMI PvuII SacI SapI SfcI SfoI SmaI StyI
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BfrBI BglII BmgBI BmtI BpuEI
BsaI BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EcoRI EcoRV FseI FspI FspAI HincII
HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XhoI ZraI