Restriction map for
pF1KB7414
(Linear) (Six-base) MAPSORT of: KIBB7414.seq Check: 4131 from: 1 to: 1266
KIBB7414.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 681 849 1185 1266
Size: 318 363 168 336 81
Fragments arranged by size:
363 336 318 168 81
AleI CACnn'nnGTG
Cuts at: 0 656 1266
Size: 656 610
ApaI G_GGCC'C
Cuts at: 0 102 1266
Size: 102 1164
ApoI r'AATT_y
Cuts at: 0 1067 1266
Size: 1067 199
BanI G'GyrC_C
Cuts at: 0 17 470 1266
Size: 17 453 796
Fragments arranged by size:
796 453 17
BanII G_rGCy'C
Cuts at: 0 102 1266
Size: 102 1164
BbeI G_GCGC'C
Cuts at: 0 21 1266
Size: 21 1245
BbsI GAAGACnn'nnnn_
Cuts at: 0 873 1255 1266
Size: 873 382 11
Fragments arranged by size:
873 382 11
BbvCI CC'TCA_GC
Cuts at: 0 462 1266
Size: 462 804
Bme1580I G_kGCm'C
Cuts at: 0 102 1266
Size: 102 1164
BmrI ACTGGGnnnn_n'
Cuts at: 0 917 1001 1085 1266
Size: 917 84 84 181
Fragments arranged by size:
917 181 84 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 208 845 871 1266
Size: 142 66 637 26 395
Fragments arranged by size:
637 395 142 66 26
Bpu10I CC'TnA_GC
Cuts at: 0 462 1266
Size: 462 804
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1266
Size: 945 321
BsaI GGTCTCn'nnnn_
Cuts at: 0 183 1266
Size: 183 1083
BsaHI Gr'CG_yC
Cuts at: 0 18 1266
Size: 18 1248
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 456 1266
Size: 456 810
BseYI C'CCAG_C
Cuts at: 0 182 371 1266
Size: 182 189 895
Fragments arranged by size:
895 189 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1266
Size: 112 1154
BsmI GAATG_Cn'
Cuts at: 0 151 679 989 1114 1266
Size: 151 528 310 125 152
Fragments arranged by size:
528 310 152 151 125
Bsp1286I G_dGCh'C
Cuts at: 0 102 1266
Size: 102 1164
BsrGI T'GTAC_A
Cuts at: 0 104 1266
Size: 104 1162
BtgI C'CryG_G
Cuts at: 0 205 1266
Size: 205 1061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 666 701 1266
Size: 666 35 565
Fragments arranged by size:
666 565 35
DraI TTT'AAA
Cuts at: 0 756 1266
Size: 756 510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 208 318 681 845 849 871 1185
Size: 142 66 110 363 164 4 22 314
Cuts at: 1185 1266
Size: 81
Fragments arranged by size:
363 314 164 142 110 81 66 22
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 465 1266
Size: 465 801
EcoO109I rG'GnC_Cy
Cuts at: 0 98 99 210 351 1266
Size: 98 1 111 141 915
Fragments arranged by size:
915 141 111 98 1
EcoRI G'AATT_C
Cuts at: 0 1067 1266
Size: 1067 199
EcoRV GAT'ATC
Cuts at: 0 581 1266
Size: 581 685
HaeII r_GCGC'y
Cuts at: 0 21 1266
Size: 21 1245
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 792 824 1044 1076 1266
Size: 298 32 462 32 220 32 190
Fragments arranged by size:
462 298 220 190 32 32 32
KasI G'GCGC_C
Cuts at: 0 17 1266
Size: 17 1249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1064 1266
Size: 919 145 202
Fragments arranged by size:
919 202 145
MslI CAynn'nnrTG
Cuts at: 0 619 638 656 756 1266
Size: 619 19 18 100 510
Fragments arranged by size:
619 510 100 19 18
MspA1I CmG'CkG
Cuts at: 0 186 1205 1266
Size: 186 1019 61
Fragments arranged by size:
1019 186 61
NarI GG'CG_CC
Cuts at: 0 18 1266
Size: 18 1248
NcoI C'CATG_G
Cuts at: 0 205 1266
Size: 205 1061
PasI CC'CwG_GG
Cuts at: 0 221 1266
Size: 221 1045
PshAI GACnn'nnGTC
Cuts at: 0 1255 1266
Size: 1255 11
PspOMI G'GGCC_C
Cuts at: 0 98 1266
Size: 98 1168
PvuII CAG'CTG
Cuts at: 0 186 1205 1266
Size: 186 1019 61
Fragments arranged by size:
1019 186 61
SfoI GGC'GCC
Cuts at: 0 19 1266
Size: 19 1247
SmlI C'TyrA_G
Cuts at: 0 960 1266
Size: 960 306
SspI AAT'ATT
Cuts at: 0 528 1266
Size: 528 738
StuI AGG'CCT
Cuts at: 0 481 862 1266
Size: 481 381 404
Fragments arranged by size:
481 404 381
StyI C'CwwG_G
Cuts at: 0 205 1266
Size: 205 1061
SwaI ATTT'AAAT
Cuts at: 0 756 1266
Size: 756 510
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 76 1266
Size: 76 1190
TatI w'GTAC_w
Cuts at: 0 53 104 1266
Size: 53 51 1162
Fragments arranged by size:
1162 53 51
Tth111I GACn'n_nGTC
Cuts at: 0 432 1266
Size: 432 834
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 413 1266
Size: 171 242 853
Fragments arranged by size:
853 242 171
XmnI GAAnn'nnTTC
Cuts at: 0 946 1114 1266
Size: 946 168 152
Fragments arranged by size:
946 168 152
Enzymes that do cut:
AcuI AleI ApaI ApoI BanI BanII BbeI BbsI
BbvCI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI
BseRI BseYI BsgI BsmI Bsp1286I BsrGI BtgI CspCI
DraI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I
KasI MmeI MslI MspA1I NarI NcoI PasI PshAI
PspOMI PvuII SfoI SmlI SspI StuI StyI SwaI
TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaAI
BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI DraIII DrdI EaeI EagI EarI EciI EcoICRI
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI TstI XbaI XhoI XmaI ZraI