Restriction map for
pF1KB7415
(Linear) (Six-base) MAPSORT of: KIBB7415.seq Check: 1212 from: 1 to: 963
KIBB7415.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 963
Size: 102 861
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 403 435 963
Size: 403 32 528
Fragments arranged by size:
528 403 32
AlwNI CAG_nnn'CTG
Cuts at: 0 169 963
Size: 169 794
ApoI r'AATT_y
Cuts at: 0 177 246 272 293 963
Size: 177 69 26 21 670
Fragments arranged by size:
670 177 69 26 21
AvaI C'yCGr_G
Cuts at: 0 664 963
Size: 664 299
BanII G_rGCy'C
Cuts at: 0 816 963
Size: 816 147
BbsI GAAGACnn'nnnn_
Cuts at: 0 570 963
Size: 570 393
BfrBI ATG'CAT
Cuts at: 0 388 963
Size: 388 575
Bme1580I G_kGCm'C
Cuts at: 0 514 611 963
Size: 514 97 352
Fragments arranged by size:
514 352 97
BmtI G_CTAG'C
Cuts at: 0 761 963
Size: 761 202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 963
Size: 128 835
Bpu10I CC'TnA_GC
Cuts at: 0 155 963
Size: 155 808
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 963
Size: 394 569
BsaI GGTCTCn'nnnn_
Cuts at: 0 110 305 963
Size: 110 195 658
Fragments arranged by size:
658 195 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 403 433 963
Size: 403 30 530
Fragments arranged by size:
530 403 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 963
Size: 391 572
BseYI C'CCAG_C
Cuts at: 0 513 963
Size: 513 450
BsiHKAI G_wGCw'C
Cuts at: 0 816 963
Size: 816 147
BsmI GAATG_Cn'
Cuts at: 0 388 963
Size: 388 575
BsmBI CGTCTCn'nnnn_
Cuts at: 0 14 963
Size: 14 949
Bsp1286I G_dGCh'C
Cuts at: 0 514 611 816 963
Size: 514 97 205 147
Fragments arranged by size:
514 205 147 97
BspHI T'CATG_A
Cuts at: 0 108 963
Size: 108 855
BstAPI GCAn_nnn'nTGC
Cuts at: 0 633 963
Size: 633 330
BstBI TT'CG_AA
Cuts at: 0 291 963
Size: 291 672
BstXI CCAn_nnnn'nTGG
Cuts at: 0 442 644 963
Size: 442 202 319
Fragments arranged by size:
442 319 202
BtsI GCAGTG_nn'
Cuts at: 0 838 878 963
Size: 838 40 85
Fragments arranged by size:
838 85 40
DraI TTT'AAA
Cuts at: 0 542 963
Size: 542 421
EaeI y'GGCC_r
Cuts at: 0 203 255 963
Size: 203 52 708
Fragments arranged by size:
708 203 52
EarI CTCTTCn'nnn_
Cuts at: 0 821 963
Size: 821 142
EcoICRI GAG'CTC
Cuts at: 0 814 963
Size: 814 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 128 963
Size: 102 26 835
Fragments arranged by size:
835 102 26
EcoO109I rG'GnC_Cy
Cuts at: 0 151 963
Size: 151 812
EcoRI G'AATT_C
Cuts at: 0 272 963
Size: 272 691
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 36 68 124 156 785 817 963
Size: 36 32 56 32 629 32 146
Fragments arranged by size:
629 146 56 36 32 32 32
MscI TGG'CCA
Cuts at: 0 205 257 963
Size: 205 52 706
Fragments arranged by size:
706 205 52
MslI CAynn'nnrTG
Cuts at: 0 440 963
Size: 440 523
NheI G'CTAG_C
Cuts at: 0 757 963
Size: 757 206
NsiI A_TGCA'T
Cuts at: 0 390 963
Size: 390 573
NspI r_CATG'y
Cuts at: 0 69 963
Size: 69 894
PasI CC'CwG_GG
Cuts at: 0 644 963
Size: 644 319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 403 435 963
Size: 403 32 528
Fragments arranged by size:
528 403 32
PpuMI rG'GwC_Cy
Cuts at: 0 151 963
Size: 151 812
SacI G_AGCT'C
Cuts at: 0 816 963
Size: 816 147
SapI GCTCTTCn'nnn_
Cuts at: 0 821 963
Size: 821 142
SmlI C'TyrA_G
Cuts at: 0 373 963
Size: 373 590
SphI G_CATG'C
Cuts at: 0 69 963
Size: 69 894
SspI AAT'ATT
Cuts at: 0 873 963
Size: 873 90
StuI AGG'CCT
Cuts at: 0 371 963
Size: 371 592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 265 963
Size: 265 698
Enzymes that do cut:
AcuI AloI AlwNI ApoI AvaI BanII BbsI BfrBI
Bme1580I BmtI BpmI Bpu10I BpuEI BsaI BsaXI BseRI
BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BstAPI BstBI
BstXI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI Hin4I MscI MslI NheI NsiI NspI PasI
PpiI PpuMI SacI SapI SmlI SphI SspI StuI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI BmgBI BmrI BplI
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NruI PacI PciI PflMI PfoI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI