Restriction map for
pF1KB7416
(Linear) (Six-base) MAPSORT of: KIBB7416.seq Check: 9634 from: 1 to: 1401
KIBB7416.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AatII G_ACGT'C
Cuts at: 0 170 1401
Size: 170 1231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 681 888 1246 1401
Size: 681 207 358 155
Fragments arranged by size:
681 358 207 155
AfeI AGC'GCT
Cuts at: 0 707 1401
Size: 707 694
AflIII A'CryG_T
Cuts at: 0 1235 1287 1401
Size: 1235 52 114
Fragments arranged by size:
1235 114 52
AleI CACnn'nnGTG
Cuts at: 0 1048 1351 1401
Size: 1048 303 50
Fragments arranged by size:
1048 303 50
AlwNI CAG_nnn'CTG
Cuts at: 0 126 995 1074 1131 1261 1401
Size: 126 869 79 57 130 140
Fragments arranged by size:
869 140 130 126 79 57
ApaI G_GGCC'C
Cuts at: 0 597 945 1401
Size: 597 348 456
Fragments arranged by size:
597 456 348
AvaI C'yCGr_G
Cuts at: 0 35 589 719 1320 1401
Size: 35 554 130 601 81
Fragments arranged by size:
601 554 130 81 35
BamHI G'GATC_C
Cuts at: 0 628 1265 1401
Size: 628 637 136
Fragments arranged by size:
637 628 136
BanI G'GyrC_C
Cuts at: 0 226 800 1401
Size: 226 574 601
Fragments arranged by size:
601 574 226
BanII G_rGCy'C
Cuts at: 0 597 920 945 1327 1361 1401
Size: 597 323 25 382 34 40
Fragments arranged by size:
597 382 323 40 34 25
BbeI G_GCGC'C
Cuts at: 0 230 1401
Size: 230 1171
BbvCI CC'TCA_GC
Cuts at: 0 502 988 1401
Size: 502 486 413
Fragments arranged by size:
502 486 413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 150 184 1401
Size: 150 34 1217
Fragments arranged by size:
1217 150 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1252 1401
Size: 1252 149
Bme1580I G_kGCm'C
Cuts at: 0 597 945 1401
Size: 597 348 456
Fragments arranged by size:
597 456 348
BmrI ACTGGGnnnn_n'
Cuts at: 0 852 1104 1401
Size: 852 252 297
Fragments arranged by size:
852 297 252
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1003 1401
Size: 1003 398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 943 1153 1294 1401
Size: 628 315 210 141 107
Fragments arranged by size:
628 315 210 141 107
Bpu10I CC'TnA_GC
Cuts at: 0 502 988 1401
Size: 502 486 413
Fragments arranged by size:
502 486 413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 1401
Size: 763 638
BsaAI yAC'GTr
Cuts at: 0 1304 1401
Size: 1304 97
BsaHI Gr'CG_yC
Cuts at: 0 167 227 301 836 1172 1401
Size: 167 60 74 535 336 229
Fragments arranged by size:
535 336 229 167 74 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 676 706 1379 1401
Size: 676 30 673 22
Fragments arranged by size:
676 673 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 220 223 1024 1401
Size: 220 3 801 377
Fragments arranged by size:
801 377 220 3
BseYI C'CCAG_C
Cuts at: 0 708 808 1175 1261 1327 1401
Size: 708 100 367 86 66 74
Fragments arranged by size:
708 367 100 86 74 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 202 259 686 1165 1319 1401
Size: 193 9 57 427 479 154 82
Fragments arranged by size:
479 427 193 154 82 57 9
BsiEI CG_ry'CG
Cuts at: 0 30 1401
Size: 30 1371
BsiHKAI G_wGCw'C
Cuts at: 0 653 920 1401
Size: 653 267 481
Fragments arranged by size:
653 481 267
BsmI GAATG_Cn'
Cuts at: 0 858 1401
Size: 858 543
BsmBI CGTCTCn'nnnn_
Cuts at: 0 204 292 1278 1401
Size: 204 88 986 123
Fragments arranged by size:
986 204 123 88
Bsp1286I G_dGCh'C
Cuts at: 0 597 653 920 945 1327 1361 1401
Size: 597 56 267 25 382 34 40
Fragments arranged by size:
597 382 267 56 40 34 25
BspHI T'CATG_A
Cuts at: 0 653 848 1196 1401
Size: 653 195 348 205
Fragments arranged by size:
653 348 205 195
BsrBI CCG'CTC
Cuts at: 0 69 1401
Size: 69 1332
BsrDI GCAATG_nn'
Cuts at: 0 542 1401
Size: 542 859
BsrFI r'CCGG_y
Cuts at: 0 7 348 440 1401
Size: 7 341 92 961
Fragments arranged by size:
961 341 92 7
BsrGI T'GTAC_A
Cuts at: 0 1238 1401
Size: 1238 163
BssSI C'ACGA_G
Cuts at: 0 790 1401
Size: 790 611
BstEII G'GTnAC_C
Cuts at: 0 132 1401
Size: 132 1269
BstXI CCAn_nnnn'nTGG
Cuts at: 0 378 1050 1353 1401
Size: 378 672 303 48
Fragments arranged by size:
672 378 303 48
BstYI r'GATC_y
Cuts at: 0 628 821 1265 1401
Size: 628 193 444 136
Fragments arranged by size:
628 444 193 136
Bsu36I CC'TnA_GG
Cuts at: 0 343 1401
Size: 343 1058
BtgI C'CryG_G
Cuts at: 0 860 932 1118 1312 1401
Size: 860 72 186 194 89
Fragments arranged by size:
860 194 186 89 72
BtsI GCAGTG_nn'
Cuts at: 0 181 1401
Size: 181 1220
DraIII CAC_nnn'GTG
Cuts at: 0 153 1401
Size: 153 1248
DrdI GACnn_nn'nnGTC
Cuts at: 0 538 1401
Size: 538 863
EaeI y'GGCC_r
Cuts at: 0 2 27 289 380 765 1401
Size: 2 25 262 91 385 636
Fragments arranged by size:
636 385 262 91 25 2
EagI C'GGCC_G
Cuts at: 0 27 1401
Size: 27 1374
EarI CTCTTCn'nnn_
Cuts at: 0 1276 1401
Size: 1276 125
EcoICRI GAG'CTC
Cuts at: 0 918 1401
Size: 918 483
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 681 888 943 1153 1246 1294 1401
Size: 628 53 207 55 210 93 48 107
Fragments arranged by size:
628 210 207 107 93 55 53 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1215 1255 1401
Size: 1215 40 146
Fragments arranged by size:
1215 146 40
EcoO109I rG'GnC_Cy
Cuts at: 0 90 213 407 471 581 593 594 716
Size: 90 123 194 64 110 12 1 122
Cuts at: 716 927 1038 1245 1401
Size: 211 111 207 156
Fragments arranged by size:
211 207 194 156 123 122 111 110
90 64 12 1
HaeII r_GCGC'y
Cuts at: 0 126 230 709 1401
Size: 126 104 479 692
Fragments arranged by size:
692 479 126 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 36 68 1401
Size: 36 32 1333
Fragments arranged by size:
1333 36 32
HincII GTy'rAC
Cuts at: 0 1101 1401
Size: 1101 300
KasI G'GCGC_C
Cuts at: 0 226 1401
Size: 226 1175
MluI A'CGCG_T
Cuts at: 0 1287 1401
Size: 1287 114
MscI TGG'CCA
Cuts at: 0 382 1401
Size: 382 1019
MslI CAynn'nnrTG
Cuts at: 0 1048 1157 1189 1195 1351 1401
Size: 1048 109 32 6 156 50
Fragments arranged by size:
1048 156 109 50 32 6
MspA1I CmG'CkG
Cuts at: 0 261 501 1179 1272 1366 1401
Size: 261 240 678 93 94 35
Fragments arranged by size:
678 261 240 94 93 35
NaeI GCC'GGC
Cuts at: 0 9 350 1401
Size: 9 341 1051
Fragments arranged by size:
1051 341 9
NarI GG'CG_CC
Cuts at: 0 227 1401
Size: 227 1174
NcoI C'CATG_G
Cuts at: 0 1118 1401
Size: 1118 283
NgoMIV G'CCGG_C
Cuts at: 0 7 348 1401
Size: 7 341 1053
Fragments arranged by size:
1053 341 7
NspI r_CATG'y
Cuts at: 0 87 1007 1194 1239 1401
Size: 87 920 187 45 162
Fragments arranged by size:
920 187 162 87 45
PasI CC'CwG_GG
Cuts at: 0 1378 1401
Size: 1378 23
PciI A'CATG_T
Cuts at: 0 1235 1401
Size: 1235 166
PflMI CCAn_nnn'nTGG
Cuts at: 0 951 1401
Size: 951 450
PfoI T'CCnGG_A
Cuts at: 0 911 920 1163 1401
Size: 911 9 243 238
Fragments arranged by size:
911 243 238 9
PpuMI rG'GwC_Cy
Cuts at: 0 90 407 471 716 927 1038 1401
Size: 90 317 64 245 211 111 363
Fragments arranged by size:
363 317 245 211 111 90 64
PspOMI G'GGCC_C
Cuts at: 0 593 941 1401
Size: 593 348 460
Fragments arranged by size:
593 460 348
PspXI vC'TCGA_Gb
Cuts at: 0 1320 1401
Size: 1320 81
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1054 1086 1401
Size: 1054 32 315
Fragments arranged by size:
1054 315 32
PstI C_TGCA'G
Cuts at: 0 357 1401
Size: 357 1044
PvuII CAG'CTG
Cuts at: 0 501 1179 1401
Size: 501 678 222
Fragments arranged by size:
678 501 222
SacI G_AGCT'C
Cuts at: 0 920 1401
Size: 920 481
SanDI GG'GwC_CC
Cuts at: 0 407 1401
Size: 407 994
ScaI AGT'ACT
Cuts at: 0 694 1401
Size: 694 707
SexAI A'CCwGG_T
Cuts at: 0 473 1401
Size: 473 928
SfcI C'TryA_G
Cuts at: 0 306 353 1401
Size: 306 47 1048
Fragments arranged by size:
1048 306 47
SfoI GGC'GCC
Cuts at: 0 228 1401
Size: 228 1173
SmaI CCC'GGG
Cuts at: 0 37 591 1401
Size: 37 554 810
Fragments arranged by size:
810 554 37
SmlI C'TyrA_G
Cuts at: 0 742 1320 1401
Size: 742 578 81
Fragments arranged by size:
742 578 81
SphI G_CATG'C
Cuts at: 0 87 1401
Size: 87 1314
StyI C'CwwG_G
Cuts at: 0 1118 1216 1401
Size: 1118 98 185
Fragments arranged by size:
1118 185 98
TatI w'GTAC_w
Cuts at: 0 692 1238 1401
Size: 692 546 163
Fragments arranged by size:
692 546 163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 126 158 1379 1401
Size: 126 32 1221 22
Fragments arranged by size:
1221 126 32 22
XhoI C'TCGA_G
Cuts at: 0 1320 1401
Size: 1320 81
XmaI C'CCGG_G
Cuts at: 0 35 589 1401
Size: 35 554 812
Fragments arranged by size:
812 554 35
ZraI GAC'GTC
Cuts at: 0 168 1401
Size: 168 1233
Enzymes that do cut:
AatII AcuI AfeI AflIII AleI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BglI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KasI MluI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PpuMI PspOMI
PspXI PsrI PstI PvuII SacI SanDI ScaI SexAI
SfcI SfoI SmaI SmlI SphI StyI TatI TstI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BsaI BsaBI BsaWI BsiWI BspEI BspMI BssHII BstAPI
BstBI BstZ17I BtgZI ClaI CspCI DraI EciI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MmeI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PvuI RsrII SacII
SalI SapI SbfI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI
XmnI