Restriction map for
pF1KB7418
(Linear) (Six-base) MAPSORT of: KIBB7418.seq Check: 2388 from: 1 to: 1131
KIBB7418.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1105 1131
Size: 1105 26
AlwNI CAG_nnn'CTG
Cuts at: 0 519 759 1131
Size: 519 240 372
Fragments arranged by size:
519 372 240
ApaI G_GGCC'C
Cuts at: 0 7 116 441 1131
Size: 7 109 325 690
Fragments arranged by size:
690 325 109 7
ApaLI G'TGCA_C
Cuts at: 0 795 1131
Size: 795 336
ApoI r'AATT_y
Cuts at: 0 1096 1131
Size: 1096 35
AvaI C'yCGr_G
Cuts at: 0 115 1131
Size: 115 1016
BanI G'GyrC_C
Cuts at: 0 255 312 451 1131
Size: 255 57 139 680
Fragments arranged by size:
680 255 139 57
BanII G_rGCy'C
Cuts at: 0 7 116 171 441 854 1131
Size: 7 109 55 270 413 277
Fragments arranged by size:
413 277 270 109 55 7
BbeI G_GCGC'C
Cuts at: 0 259 1131
Size: 259 872
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 786 820 1131
Size: 786 34 311
Fragments arranged by size:
786 311 34
BfrBI ATG'CAT
Cuts at: 0 577 1131
Size: 577 554
BglI GCCn_nnn'nGGC
Cuts at: 0 96 253 722 1131
Size: 96 157 469 409
Fragments arranged by size:
469 409 157 96
BlpI GC'TnA_GC
Cuts at: 0 928 1131
Size: 928 203
Bme1580I G_kGCm'C
Cuts at: 0 7 116 441 735 799 1131
Size: 7 109 325 294 64 332
Fragments arranged by size:
332 325 294 109 64 7
BmgBI CAC'GTC
Cuts at: 0 189 1131
Size: 189 942
BmrI ACTGGGnnnn_n'
Cuts at: 0 360 1131
Size: 360 771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1057 1131
Size: 241 816 74
Fragments arranged by size:
816 241 74
Bpu10I CC'TnA_GC
Cuts at: 0 523 1131
Size: 523 608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 1131
Size: 892 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 961 1131
Size: 961 170
BsaAI yAC'GTr
Cuts at: 0 345 1131
Size: 345 786
BsaBI GATnn'nnATC
Cuts at: 0 289 1131
Size: 289 842
BsaHI Gr'CG_yC
Cuts at: 0 227 256 829 1131
Size: 227 29 573 302
Fragments arranged by size:
573 302 227 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 848 878 995 1025 1105 1131
Size: 848 30 117 30 80 26
Fragments arranged by size:
848 117 80 30 30 26
BseYI C'CCAG_C
Cuts at: 0 99 264 512 818 1131
Size: 99 165 248 306 313
Fragments arranged by size:
313 306 248 165 99
BsiEI CG_ry'CG
Cuts at: 0 149 273 1131
Size: 149 124 858
Fragments arranged by size:
858 149 124
BsiHKAI G_wGCw'C
Cuts at: 0 799 854 1131
Size: 799 55 277
Fragments arranged by size:
799 277 55
BsmBI CGTCTCn'nnnn_
Cuts at: 0 65 1131
Size: 65 1066
Bsp1286I G_dGCh'C
Cuts at: 0 7 116 171 441 735 799 854 1131
Size: 7 109 55 270 294 64 55 277
Fragments arranged by size:
294 277 270 109 64 55 55 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1090 1131
Size: 1090 41
BsrBI CCG'CTC
Cuts at: 0 13 1131
Size: 13 1118
BsrDI GCAATG_nn'
Cuts at: 0 577 1131
Size: 577 554
BsrFI r'CCGG_y
Cuts at: 0 141 923 1131
Size: 141 782 208
Fragments arranged by size:
782 208 141
BssSI C'ACGA_G
Cuts at: 0 805 1033 1131
Size: 805 228 98
Fragments arranged by size:
805 228 98
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 570 777 1131
Size: 417 153 207 354
Fragments arranged by size:
417 354 207 153
BstYI r'GATC_y
Cuts at: 0 954 1131
Size: 954 177
BtgI C'CryG_G
Cuts at: 0 122 599 1131
Size: 122 477 532
Fragments arranged by size:
532 477 122
BtsI GCAGTG_nn'
Cuts at: 0 341 396 1131
Size: 341 55 735
Fragments arranged by size:
735 341 55
DraIII CAC_nnn'GTG
Cuts at: 0 489 1131
Size: 489 642
DrdI GACnn_nn'nnGTC
Cuts at: 0 214 1131
Size: 214 917
EaeI y'GGCC_r
Cuts at: 0 146 782 1131
Size: 146 636 349
Fragments arranged by size:
636 349 146
EagI C'GGCC_G
Cuts at: 0 146 1131
Size: 146 985
EarI CTCTTCn'nnn_
Cuts at: 0 448 1131
Size: 448 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 35 142 179 1131
Size: 35 107 37 952
Fragments arranged by size:
952 107 37 35
EcoICRI GAG'CTC
Cuts at: 0 852 1131
Size: 852 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1057 1131
Size: 241 816 74
Fragments arranged by size:
816 241 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 679 1131
Size: 679 452
EcoO109I rG'GnC_Cy
Cuts at: 0 4 112 414 438 590 945 1131
Size: 4 108 302 24 152 355 186
Fragments arranged by size:
355 302 186 152 108 24 4
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 672 1131
Size: 672 459
HaeII r_GCGC'y
Cuts at: 0 259 882 921 1131
Size: 259 623 39 210
Fragments arranged by size:
623 259 210 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 613 645 1131
Size: 613 32 486
Fragments arranged by size:
613 486 32
HincII GTy'rAC
Cuts at: 0 653 1131
Size: 653 478
KasI G'GCGC_C
Cuts at: 0 255 1131
Size: 255 876
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 1131
Size: 551 580
MscI TGG'CCA
Cuts at: 0 784 1131
Size: 784 347
MspA1I CmG'CkG
Cuts at: 0 66 93 124 617 759 822 846 1131
Size: 66 27 31 493 142 63 24 285
Fragments arranged by size:
493 285 142 66 63 31 27 24
NaeI GCC'GGC
Cuts at: 0 143 925 1131
Size: 143 782 206
Fragments arranged by size:
782 206 143
NarI GG'CG_CC
Cuts at: 0 256 1131
Size: 256 875
NgoMIV G'CCGG_C
Cuts at: 0 141 923 1131
Size: 141 782 208
Fragments arranged by size:
782 208 141
NsiI A_TGCA'T
Cuts at: 0 579 1131
Size: 579 552
PflMI CCAn_nnn'nTGG
Cuts at: 0 489 1131
Size: 489 642
PfoI T'CCnGG_A
Cuts at: 0 218 1131
Size: 218 913
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1105 1131
Size: 1105 26
PpuMI rG'GwC_Cy
Cuts at: 0 590 1131
Size: 590 541
PspOMI G'GGCC_C
Cuts at: 0 3 112 437 1131
Size: 3 109 325 694
Fragments arranged by size:
694 325 109 3
PstI C_TGCA'G
Cuts at: 0 587 755 1131
Size: 587 168 376
Fragments arranged by size:
587 376 168
PvuII CAG'CTG
Cuts at: 0 759 1131
Size: 759 372
SacI G_AGCT'C
Cuts at: 0 854 1131
Size: 854 277
SacII CC_GC'GG
Cuts at: 0 125 1131
Size: 125 1006
SfcI C'TryA_G
Cuts at: 0 583 751 1131
Size: 583 168 380
Fragments arranged by size:
583 380 168
SfoI GGC'GCC
Cuts at: 0 257 1131
Size: 257 874
SmaI CCC'GGG
Cuts at: 0 117 1131
Size: 117 1014
SmlI C'TyrA_G
Cuts at: 0 907 1131
Size: 907 224
StuI AGG'CCT
Cuts at: 0 403 803 1131
Size: 403 400 328
Fragments arranged by size:
403 400 328
StyI C'CwwG_G
Cuts at: 0 410 680 717 1131
Size: 410 270 37 414
Fragments arranged by size:
414 410 270 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 439 1131
Size: 439 692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 654 686 848 880 1131
Size: 654 32 162 32 251
Fragments arranged by size:
654 251 162 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 709 1131
Size: 709 422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 323 777 1131
Size: 323 454 354
Fragments arranged by size:
454 354 323
XmaI C'CCGG_G
Cuts at: 0 115 1131
Size: 115 1016
Enzymes that do cut:
AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII
BbeI BcgI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstXI BstYI BtgI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI MmeI MscI MspA1I
NaeI NarI NgoMIV NsiI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII SacI SacII SfcI SfoI SmaI
SmlI StuI StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BclI BglII
BmtI BplI BsaWI BseRI BsgI BsiWI BsmI BspEI
BspHI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MslI NcoI
NdeI NheI NotI NruI NspI PacI PasI PciI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
XbaI XhoI XmnI ZraI