Restriction map for
pF1KB7419
(Linear) (Six-base) MAPSORT of: KIBB7419.seq Check: 8275 from: 1 to: 954
KIBB7419.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
Acc65I G'GTAC_C
Cuts at: 0 757 954
Size: 757 197
ApoI r'AATT_y
Cuts at: 0 31 954
Size: 31 923
AseI AT'TA_AT
Cuts at: 0 521 954
Size: 521 433
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 422 455 954
Size: 422 33 499
Fragments arranged by size:
499 422 33
BanI G'GyrC_C
Cuts at: 0 757 954
Size: 757 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 154 705 954
Size: 154 551 249
Fragments arranged by size:
551 249 154
BclI T'GATC_A
Cuts at: 0 134 743 954
Size: 134 609 211
Fragments arranged by size:
609 211 134
BglII A'GATC_T
Cuts at: 0 539 954
Size: 539 415
BlpI GC'TnA_GC
Cuts at: 0 723 931 954
Size: 723 208 23
Fragments arranged by size:
723 208 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 912 954
Size: 88 824 42
Fragments arranged by size:
824 88 42
BsaAI yAC'GTr
Cuts at: 0 105 954
Size: 105 849
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 216 954
Size: 216 738
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 316 954
Size: 311 5 638
Fragments arranged by size:
638 311 5
BspHI T'CATG_A
Cuts at: 0 404 954
Size: 404 550
BsrDI GCAATG_nn'
Cuts at: 0 129 323 954
Size: 129 194 631
Fragments arranged by size:
631 194 129
BssSI C'ACGA_G
Cuts at: 0 634 954
Size: 634 320
BstEII G'GTnAC_C
Cuts at: 0 55 954
Size: 55 899
BstYI r'GATC_y
Cuts at: 0 539 954
Size: 539 415
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 507 954
Size: 507 447
BtsI GCAGTG_nn'
Cuts at: 0 299 605 954
Size: 299 306 349
Fragments arranged by size:
349 306 299
EaeI y'GGCC_r
Cuts at: 0 371 689 954
Size: 371 318 265
Fragments arranged by size:
371 318 265
EcoO109I rG'GnC_Cy
Cuts at: 0 795 954
Size: 795 159
EcoRI G'AATT_C
Cuts at: 0 31 954
Size: 31 923
EcoRV GAT'ATC
Cuts at: 0 210 954
Size: 210 744
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 954
Size: 383 32 539
Fragments arranged by size:
539 383 32
HincII GTy'rAC
Cuts at: 0 326 954
Size: 326 628
KpnI G_GTAC'C
Cuts at: 0 761 954
Size: 761 193
MscI TGG'CCA
Cuts at: 0 373 954
Size: 373 581
MslI CAynn'nnrTG
Cuts at: 0 172 403 954
Size: 172 231 551
Fragments arranged by size:
551 231 172
NspI r_CATG'y
Cuts at: 0 171 722 954
Size: 171 551 232
Fragments arranged by size:
551 232 171
PflMI CCAn_nnn'nTGG
Cuts at: 0 494 954
Size: 494 460
PfoI T'CCnGG_A
Cuts at: 0 203 954
Size: 203 751
PpuMI rG'GwC_Cy
Cuts at: 0 795 954
Size: 795 159
SfcI C'TryA_G
Cuts at: 0 824 885 954
Size: 824 61 69
Fragments arranged by size:
824 69 61
SmlI C'TyrA_G
Cuts at: 0 67 891 954
Size: 67 824 63
Fragments arranged by size:
824 67 63
SnaBI TAC'GTA
Cuts at: 0 105 954
Size: 105 849
SspI AAT'ATT
Cuts at: 0 516 585 609 925 954
Size: 516 69 24 316 29
Fragments arranged by size:
516 316 69 29 24
StyI C'CwwG_G
Cuts at: 0 342 801 954
Size: 342 459 153
Fragments arranged by size:
459 342 153
TatI w'GTAC_w
Cuts at: 0 176 423 954
Size: 176 247 531
Fragments arranged by size:
531 247 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 278 310 954
Size: 278 32 644
Fragments arranged by size:
644 278 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 799 954
Size: 799 155
XmnI GAAnn'nnTTC
Cuts at: 0 35 273 462 954
Size: 35 238 189 492
Fragments arranged by size:
492 238 189 35
Enzymes that do cut:
Acc65I ApoI AseI BaeI BanI BbsI BclI BglII
BlpI BpuEI BsaAI BseRI BsgI BspHI BsrDI BssSI
BstEII BstYI BtgZI BtsI EaeI EcoO109I EcoRI EcoRV
Hin4I HincII KpnI MscI MslI NspI PflMI PfoI
PpuMI SfcI SmlI SnaBI SspI StyI TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AsiSI AvaI AvrII BamHI BanII BbeI BbvCI
BcgI BciVI BfrBI BglI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI Eco57MI EcoNI FalI FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI
SphI SrfI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI ZraI