Restriction map for
pF1KB7420
(Linear) (Six-base) MAPSORT of: KIBB7420.seq Check: 168 from: 1 to: 807
KIBB7420.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 386 807
Size: 386 421
AfeI AGC'GCT
Cuts at: 0 168 807
Size: 168 639
AlwNI CAG_nnn'CTG
Cuts at: 0 799 807
Size: 799 8
AscI GG'CGCG_CC
Cuts at: 0 150 807
Size: 150 657
BanI G'GyrC_C
Cuts at: 0 369 536 629 807
Size: 369 167 93 178
Fragments arranged by size:
369 178 167 93
BanII G_rGCy'C
Cuts at: 0 614 807
Size: 614 193
BbeI G_GCGC'C
Cuts at: 0 633 807
Size: 633 174
BbsI GAAGACnn'nnnn_
Cuts at: 0 417 807
Size: 417 390
BbvCI CC'TCA_GC
Cuts at: 0 511 807
Size: 511 296
BglII A'GATC_T
Cuts at: 0 36 807
Size: 36 771
Bme1580I G_kGCm'C
Cuts at: 0 374 539 807
Size: 374 165 268
Fragments arranged by size:
374 268 165
BmrI ACTGGGnnnn_n'
Cuts at: 0 9 537 807
Size: 9 528 270
Fragments arranged by size:
528 270 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 379 391 628 807
Size: 155 224 12 237 179
Fragments arranged by size:
237 224 179 155 12
Bpu10I CC'TnA_GC
Cuts at: 0 511 807
Size: 511 296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 685 807
Size: 619 66 122
Fragments arranged by size:
619 122 66
BsaBI GATnn'nnATC
Cuts at: 0 766 807
Size: 766 41
BsaHI Gr'CG_yC
Cuts at: 0 307 630 807
Size: 307 323 177
Fragments arranged by size:
323 307 177
BsaWI w'CCGG_w
Cuts at: 0 50 807
Size: 50 757
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 807
Size: 187 30 590
Fragments arranged by size:
590 187 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 807
Size: 274 533
BsiHKAI G_wGCw'C
Cuts at: 0 614 807
Size: 614 193
Bsp1286I G_dGCh'C
Cuts at: 0 374 539 614 807
Size: 374 165 75 193
Fragments arranged by size:
374 193 165 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 386 807
Size: 386 421
BsrFI r'CCGG_y
Cuts at: 0 456 807
Size: 456 351
BssHII G'CGCG_C
Cuts at: 0 150 807
Size: 150 657
BstXI CCAn_nnnn'nTGG
Cuts at: 0 22 807
Size: 22 785
BstYI r'GATC_y
Cuts at: 0 36 386 807
Size: 36 350 421
Fragments arranged by size:
421 350 36
BtgI C'CryG_G
Cuts at: 0 434 807
Size: 434 373
BtsI GCAGTG_nn'
Cuts at: 0 213 807
Size: 213 594
DrdI GACnn_nn'nnGTC
Cuts at: 0 703 807
Size: 703 104
EaeI y'GGCC_r
Cuts at: 0 431 489 807
Size: 431 58 318
Fragments arranged by size:
431 318 58
EarI CTCTTCn'nnn_
Cuts at: 0 736 807
Size: 736 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 232 807
Size: 232 575
EcoICRI GAG'CTC
Cuts at: 0 612 807
Size: 612 195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 379 391 628 807
Size: 155 224 12 237 179
Fragments arranged by size:
237 224 179 155 12
EcoO109I rG'GnC_Cy
Cuts at: 0 572 783 807
Size: 572 211 24
Fragments arranged by size:
572 211 24
HaeII r_GCGC'y
Cuts at: 0 170 633 807
Size: 170 463 174
Fragments arranged by size:
463 174 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 404 436 807
Size: 404 32 371
Fragments arranged by size:
404 371 32
HincII GTy'rAC
Cuts at: 0 114 399 807
Size: 114 285 408
Fragments arranged by size:
408 285 114
KasI G'GCGC_C
Cuts at: 0 629 807
Size: 629 178
MscI TGG'CCA
Cuts at: 0 433 491 807
Size: 433 58 316
Fragments arranged by size:
433 316 58
MslI CAynn'nnrTG
Cuts at: 0 439 559 807
Size: 439 120 248
Fragments arranged by size:
439 248 120
MspA1I CmG'CkG
Cuts at: 0 88 807
Size: 88 719
NaeI GCC'GGC
Cuts at: 0 458 807
Size: 458 349
NarI GG'CG_CC
Cuts at: 0 630 807
Size: 630 177
NgoMIV G'CCGG_C
Cuts at: 0 456 807
Size: 456 351
NspI r_CATG'y
Cuts at: 0 324 444 807
Size: 324 120 363
Fragments arranged by size:
363 324 120
PpuMI rG'GwC_Cy
Cuts at: 0 572 783 807
Size: 572 211 24
Fragments arranged by size:
572 211 24
PshAI GACnn'nnGTC
Cuts at: 0 781 807
Size: 781 26
SacI G_AGCT'C
Cuts at: 0 614 807
Size: 614 193
SanDI GG'GwC_CC
Cuts at: 0 572 807
Size: 572 235
SapI GCTCTTCn'nnn_
Cuts at: 0 736 807
Size: 736 71
SfoI GGC'GCC
Cuts at: 0 631 807
Size: 631 176
SmlI C'TyrA_G
Cuts at: 0 634 664 807
Size: 634 30 143
Fragments arranged by size:
634 143 30
StyI C'CwwG_G
Cuts at: 0 486 807
Size: 486 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 807
Size: 595 212
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 187 219 807
Size: 187 32 588
Fragments arranged by size:
588 187 32
Enzymes that do cut:
AarI AfeI AlwNI AscI BanI BanII BbeI BbsI
BbvCI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI
BsaHI BsaWI BsaXI BsgI BsiHKAI Bsp1286I BspMI BsrFI
BssHII BstXI BstYI BtgI BtsI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PpuMI
PshAI SacI SanDI SapI SfoI SmlI StyI TaqII
TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmtI BplI BsaI BsaAI
BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI