(Linear) (Six-base) MAPSORT of: KIBB7422.seq  Check: 3475  from: 1  to: 408



KIBB7422.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0      31     408 
   Size:         31     377
 
AlwNI CAG_nnn'CTG

Cuts at:      0     210     408 
   Size:        210     198
 
AvaI C'yCGr_G

Cuts at:      0      48     408 
   Size:         48     360
 
BanI G'GyrC_C

Cuts at:      0     133     408 
   Size:        133     275
 
BanII G_rGCy'C

Cuts at:      0     262     408 
   Size:        262     146
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     323     408 
   Size:        323      85
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       4      38     408 
   Size:          4      34     370

  Fragments arranged by size: 

                 370      34       4
 
BglI GCCn_nnn'nGGC

Cuts at:      0      70     271     292     408 
   Size:         70     201      21     116

  Fragments arranged by size: 

                 201     116      70      21
 
BsiEI CG_ry'CG

Cuts at:      0      35     266     408 
   Size:         35     231     142

  Fragments arranged by size: 

                 231     142      35
 
BsiHKAI G_wGCw'C

Cuts at:      0     262     408 
   Size:        262     146
 
Bsp1286I G_dGCh'C

Cuts at:      0     262     408 
   Size:        262     146
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     254     408 
   Size:        254     154
 
BsrBI CCG'CTC

Cuts at:      0     402     408 
   Size:        402       6
 
BsrFI r'CCGG_y

Cuts at:      0      34      90     125     408 
   Size:         34      56      35     283

  Fragments arranged by size: 

                 283      56      35      34
 
BssHII G'CGCG_C

Cuts at:      0      86     408 
   Size:         86     322
 
BstBI TT'CG_AA

Cuts at:      0     314     408 
   Size:        314      94
 
BstYI r'GATC_y

Cuts at:      0     152     408 
   Size:        152     256
 
BtgI C'CryG_G

Cuts at:      0      95     173     393     408 
   Size:         95      78     220      15

  Fragments arranged by size: 

                 220      95      78      15
 
EaeI y'GGCC_r

Cuts at:      0      62      92     263     408 
   Size:         62      30     171     145

  Fragments arranged by size: 

                 171     145      62      30
 
EagI C'GGCC_G

Cuts at:      0     263     408 
   Size:        263     145
 
EcoICRI GAG'CTC

Cuts at:      0     260     408 
   Size:        260     148
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45     408 
   Size:         45     363
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     216     408 
   Size:        216     192
 
HaeII r_GCGC'y

Cuts at:      0     162     277     408 
   Size:        162     115     131

  Fragments arranged by size: 

                 162     131     115
 
HincII GTy'rAC

Cuts at:      0      32     408 
   Size:         32     376
 
MscI TGG'CCA

Cuts at:      0      64     408 
   Size:         64     344
 
MspA1I CmG'CkG

Cuts at:      0      60     207     378     408 
   Size:         60     147     171      30

  Fragments arranged by size: 

                 171     147      60      30
 
NaeI GCC'GGC

Cuts at:      0      92     408 
   Size:         92     316
 
NgoMIV G'CCGG_C

Cuts at:      0      90     408 
   Size:         90     318
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     210     408 
   Size:        210     198
 
PstI C_TGCA'G

Cuts at:      0     281     408 
   Size:        281     127
 
PvuII CAG'CTG

Cuts at:      0      60     378     408 
   Size:         60     318      30

  Fragments arranged by size: 

                 318      60      30
 
SacI G_AGCT'C

Cuts at:      0     262     408 
   Size:        262     146
 
SalI G'TCGA_C

Cuts at:      0      30     408 
   Size:         30     378
 
SexAI A'CCwGG_T

Cuts at:      0     299     408 
   Size:        299     109
 
SfcI C'TryA_G

Cuts at:      0     277     408 
   Size:        277     131
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     292     408 
   Size:        292     116
 
StuI AGG'CCT

Cuts at:      0     295     408 
   Size:        295     113
 
StyI C'CwwG_G

Cuts at:      0      68     365     408 
   Size:         68     297      43

  Fragments arranged by size: 

                 297      68      43
 

 Enzymes that do cut:

     AccI    AlwNI     AvaI     BanI    BanII     BbsI     BcgI     BglI
    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BstBI
    BstYI     BtgI     EaeI     EagI  EcoICRI EcoO109I     FalI    HaeII
   HincII     MscI   MspA1I     NaeI   NgoMIV    PflMI     PstI    PvuII
     SacI     SalI    SexAI     SfcI     SfiI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI    BssSI   BstAPI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EarI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII    SanDI     SapI     SbfI     ScaI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI