(Linear) (Six-base) MAPSORT of: KIBB7423.seq  Check: 6333  from: 1  to: 729



KIBB7423.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     729 
   Size:        189     540
 
AflIII A'CryG_T

Cuts at:      0      31     729 
   Size:         31     698
 
AleI CACnn'nnGTG

Cuts at:      0     436     729 
   Size:        436     293
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     454     729 
   Size:        454     275
 
AlwNI CAG_nnn'CTG

Cuts at:      0      78     729 
   Size:         78     651
 
ApaLI G'TGCA_C

Cuts at:      0     439     579     729 
   Size:        439     140     150

  Fragments arranged by size: 

                 439     150     140
 
BanI G'GyrC_C

Cuts at:      0     198     729 
   Size:        198     531
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     576     729 
   Size:        576     153
 
BclI T'GATC_A

Cuts at:      0     183     729 
   Size:        183     546
 
Bme1580I G_kGCm'C

Cuts at:      0     203     379     443     583     729 
   Size:        203     176      64     140     146

  Fragments arranged by size: 

                 203     176     146     140      64
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     532     626     729 
   Size:         86     446      94     103

  Fragments arranged by size: 

                 446     103      94      86
 
BsaAI yAC'GTr

Cuts at:      0     444     729 
   Size:        444     285
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     428     458     729 
   Size:        428      30     271

  Fragments arranged by size: 

                 428     271      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     156     161     478     504     529     556     570     729 
   Size:        156       5     317      26      25      27      14     159

  Fragments arranged by size: 

                 317     159     156      27      26      25      14       5
            
 
BseYI C'CCAG_C

Cuts at:      0     595     729 
   Size:        595     134
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     567     729 
   Size:        567     162
 
BsiHKAI G_wGCw'C

Cuts at:      0     443     562     583     729 
   Size:        443     119      21     146

  Fragments arranged by size: 

                 443     146     119      21
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      62     729 
   Size:         62     667
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     379     443     562     583     729 
   Size:        203     176      64     119      21     146

  Fragments arranged by size: 

                 203     176     146     119      64      21
 
BsrDI GCAATG_nn'

Cuts at:      0     659     729 
   Size:        659      70
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     374     729 
   Size:        374     355
 
BstYI r'GATC_y

Cuts at:      0     500     729 
   Size:        500     229
 
BtsI GCAGTG_nn'

Cuts at:      0     691     729 
   Size:        691      38
 
EarI CTCTTCn'nnn_

Cuts at:      0     672     729 
   Size:        672      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     189     532     626     729 
   Size:         86     103     343      94     103

  Fragments arranged by size: 

                 343     103     103      94      86
 
HaeII r_GCGC'y

Cuts at:      0      16     729 
   Size:         16     713
 
HincII GTy'rAC

Cuts at:      0     234     729 
   Size:        234     495
 
MslI CAynn'nnrTG

Cuts at:      0      87     436     729 
   Size:         87     349     293

  Fragments arranged by size: 

                 349     293      87
 
MspA1I CmG'CkG

Cuts at:      0      78     729 
   Size:         78     651
 
NspI r_CATG'y

Cuts at:      0      35     395     729 
   Size:         35     360     334

  Fragments arranged by size: 

                 360     334      35
 
PasI CC'CwG_GG

Cuts at:      0     676     729 
   Size:        676      53
 
PciI A'CATG_T

Cuts at:      0      31     729 
   Size:         31     698
 
PfoI T'CCnGG_A

Cuts at:      0     412     729 
   Size:        412     317
 
SfcI C'TryA_G

Cuts at:      0     291     729 
   Size:        291     438
 
StyI C'CwwG_G

Cuts at:      0     177     452     729 
   Size:        177     275     277

  Fragments arranged by size: 

                 277     275     177
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     609     729 
   Size:        609     120
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    AlwNI    ApaLI     BanI     BbsI
     BclI Bme1580I     BpmI    BsaAI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BsrDI   BstAPI    BstYI     BtsI     EarI
  Eco57MI    HaeII   HincII     MslI   MspA1I     NspI     PasI     PciI
     PfoI     SfcI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI     ApaI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI