(Linear) (Six-base) MAPSORT of: KIBB7424.seq  Check: 8170  from: 1  to: 1446



KIBB7424.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     928    1297    1446 
   Size:        928     369     149

  Fragments arranged by size: 

                 928     369     149
 
ApoI r'AATT_y

Cuts at:      0     990    1008    1446 
   Size:        990      18     438

  Fragments arranged by size: 

                 990     438      18
 
AseI AT'TA_AT

Cuts at:      0    1049    1399    1446 
   Size:       1049     350      47

  Fragments arranged by size: 

                1049     350      47
 
BanII G_rGCy'C

Cuts at:      0     276    1446 
   Size:        276    1170
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     797    1446 
   Size:        797     649
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     488     522    1446 
   Size:        488      34     924

  Fragments arranged by size: 

                 924     488      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     767    1446 
   Size:        767     679
 
BclI T'GATC_A

Cuts at:      0    1026    1446 
   Size:       1026     420
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     131     133    1446 
   Size:        131       2    1313

  Fragments arranged by size: 

                1313     131       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     288     953     981    1446 
   Size:        288     665      28     465

  Fragments arranged by size: 

                 665     465     288      28
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1182    1446 
   Size:       1182     264
 
BsaBI GATnn'nnATC

Cuts at:      0      32    1343    1446 
   Size:         32    1311     103

  Fragments arranged by size: 

                1311     103      32
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1073    1103    1446 
   Size:       1073      30     343

  Fragments arranged by size: 

                1073     343      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     422    1446 
   Size:        422    1024
 
BseYI C'CCAG_C

Cuts at:      0    1077    1184    1446 
   Size:       1077     107     262

  Fragments arranged by size: 

                1077     262     107
 
Bsp1286I G_dGCh'C

Cuts at:      0     276    1446 
   Size:        276    1170
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     892     928    1446 
   Size:        892      36     518

  Fragments arranged by size: 

                 892     518      36
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     410    1446 
   Size:        410    1036
 
BtgI C'CryG_G

Cuts at:      0     251     796    1446 
   Size:        251     545     650

  Fragments arranged by size: 

                 650     545     251
 
DraI TTT'AAA

Cuts at:      0     408    1208    1446 
   Size:        408     800     238

  Fragments arranged by size: 

                 800     408     238
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      12    1446 
   Size:         12    1434
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1048    1446 
   Size:       1048     398
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     288     953     981    1446 
   Size:        288     665      28     465

  Fragments arranged by size: 

                 665     465     288      28
 
EcoRI G'AATT_C

Cuts at:      0    1008    1446 
   Size:       1008     438
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     926     958    1038    1070    1446 
   Size:        926      32      80      32     376

  Fragments arranged by size: 

                 926     376      80      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     624    1446 
   Size:        624     822
 
MslI CAynn'nnrTG

Cuts at:      0     408    1446 
   Size:        408    1038
 
NcoI C'CATG_G

Cuts at:      0     251     796    1446 
   Size:        251     545     650

  Fragments arranged by size: 

                 650     545     251
 
NspI r_CATG'y

Cuts at:      0     636     983    1446 
   Size:        636     347     463

  Fragments arranged by size: 

                 636     463     347
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1004    1446 
   Size:       1004     442
 
PsiI TTA'TAA

Cuts at:      0    1166    1446 
   Size:       1166     280
 
PstI C_TGCA'G

Cuts at:      0     501    1438    1446 
   Size:        501     937       8

  Fragments arranged by size: 

                 937     501       8
 
ScaI AGT'ACT

Cuts at:      0      57    1446 
   Size:         57    1389
 
SfcI C'TryA_G

Cuts at:      0     497     677    1434    1446 
   Size:        497     180     757      12

  Fragments arranged by size: 

                 757     497     180      12
 
SmlI C'TyrA_G

Cuts at:      0    1197    1446 
   Size:       1197     249
 
SphI G_CATG'C

Cuts at:      0     983    1446 
   Size:        983     463
 
SspI AAT'ATT

Cuts at:      0    1403    1446 
   Size:       1403      43
 
StyI C'CwwG_G

Cuts at:      0     224     251     796    1232    1446 
   Size:        224      27     545     436     214

  Fragments arranged by size: 

                 545     436     224     214      27
 
SwaI ATTT'AAAT

Cuts at:      0     408    1446 
   Size:        408    1038
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     350     579     585    1446 
   Size:        350     229       6     861

  Fragments arranged by size: 

                 861     350     229       6
 
TatI w'GTAC_w

Cuts at:      0      55     234    1446 
   Size:         55     179    1212

  Fragments arranged by size: 

                1212     179      55
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     101    1446 
   Size:        101    1345
 

 Enzymes that do cut:

    AlwNI     ApoI     AseI    BanII     BbsI     BcgI    BciVI     BclI
     BplI     BpmI    BpuEI    BsaBI    BsaXI    BseRI    BseYI Bsp1286I
   BstAPI    BstXI     BtgI     DraI     DrdI     EciI  Eco57MI    EcoRI
    Hin4I     MmeI     MslI     NcoI     NspI    PflMI     PsiI     PstI
     ScaI     SfcI     SmlI     SphI     SspI     StyI     SwaI    TaqII
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     EaeI
     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     StuI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI