Restriction map for
pF1KB7425
(Linear) (Six-base) MAPSORT of: KIBB7425.seq Check: 4437 from: 1 to: 1437
KIBB7425.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AccI GT'mk_AC
Cuts at: 0 605 1317 1437
Size: 605 712 120
Fragments arranged by size:
712 605 120
Acc65I G'GTAC_C
Cuts at: 0 944 1437
Size: 944 493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 822 985 1036 1201 1282 1437
Size: 175 647 163 51 165 81 155
Fragments arranged by size:
647 175 165 163 155 81 51
AflIII A'CryG_T
Cuts at: 0 641 1163 1437
Size: 641 522 274
Fragments arranged by size:
641 522 274
AlwNI CAG_nnn'CTG
Cuts at: 0 48 143 1437
Size: 48 95 1294
Fragments arranged by size:
1294 95 48
ApaLI G'TGCA_C
Cuts at: 0 989 1437
Size: 989 448
ApoI r'AATT_y
Cuts at: 0 330 472 579 632 772 1437
Size: 330 142 107 53 140 665
Fragments arranged by size:
665 330 142 140 107 53
AvaI C'yCGr_G
Cuts at: 0 1244 1437
Size: 1244 193
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 936 969 1437
Size: 936 33 468
Fragments arranged by size:
936 468 33
BanI G'GyrC_C
Cuts at: 0 866 944 1437
Size: 866 78 493
Fragments arranged by size:
866 493 78
BanII G_rGCy'C
Cuts at: 0 104 880 1016 1026 1286 1340 1437
Size: 104 776 136 10 260 54 97
Fragments arranged by size:
776 260 136 104 97 54 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 217 1437
Size: 217 1220
BciVI GTATCCnnnnn_n'
Cuts at: 0 628 1437
Size: 628 809
BclI T'GATC_A
Cuts at: 0 860 1437
Size: 860 577
BglI GCCn_nnn'nGGC
Cuts at: 0 1202 1437
Size: 1202 235
BglII A'GATC_T
Cuts at: 0 227 486 1437
Size: 227 259 951
Fragments arranged by size:
951 259 227
BlpI GC'TnA_GC
Cuts at: 0 739 1210 1304 1437
Size: 739 471 94 133
Fragments arranged by size:
739 471 133 94
Bme1580I G_kGCm'C
Cuts at: 0 993 1437
Size: 993 444
BmgBI CAC'GTC
Cuts at: 0 1166 1385 1437
Size: 1166 219 52
Fragments arranged by size:
1166 219 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 244 371 790 817 1423 1437
Size: 49 195 127 419 27 606 14
Fragments arranged by size:
606 419 195 127 49 27 14
Bpu10I CC'TnA_GC
Cuts at: 0 682 1117 1437
Size: 682 435 320
Fragments arranged by size:
682 435 320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1030 1437
Size: 448 582 407
Fragments arranged by size:
582 448 407
BsaBI GATnn'nnATC
Cuts at: 0 485 1437
Size: 485 952
BsaHI Gr'CG_yC
Cuts at: 0 214 1437
Size: 214 1223
BsaWI w'CCGG_w
Cuts at: 0 1359 1437
Size: 1359 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 8 38 1437
Size: 8 30 1399
Fragments arranged by size:
1399 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 778 1276 1437
Size: 778 498 161
Fragments arranged by size:
778 498 161
BseYI C'CCAG_C
Cuts at: 0 51 1437
Size: 51 1386
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1437
Size: 187 1250
BsiHKAI G_wGCw'C
Cuts at: 0 104 788 993 1026 1286 1437
Size: 104 684 205 33 260 151
Fragments arranged by size:
684 260 205 151 104 33
BsmI GAATG_Cn'
Cuts at: 0 572 1437
Size: 572 865
Bsp1286I G_dGCh'C
Cuts at: 0 104 788 880 993 1016 1026 1286 1340
Size: 104 684 92 113 23 10 260 54
Cuts at: 1340 1437
Size: 97
Fragments arranged by size:
684 260 113 104 97 92 54 23
10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 743 1196 1437
Size: 743 453 241
Fragments arranged by size:
743 453 241
BsrDI GCAATG_nn'
Cuts at: 0 1164 1437
Size: 1164 273
BstAPI GCAn_nnn'nTGC
Cuts at: 0 48 143 1437
Size: 48 95 1294
Fragments arranged by size:
1294 95 48
BstYI r'GATC_y
Cuts at: 0 227 486 1346 1437
Size: 227 259 860 91
Fragments arranged by size:
860 259 227 91
BstZ17I GTA'TAC
Cuts at: 0 606 1318 1437
Size: 606 712 119
Fragments arranged by size:
712 606 119
Bsu36I CC'TnA_GG
Cuts at: 0 790 871 1437
Size: 790 81 566
Fragments arranged by size:
790 566 81
BtgI C'CryG_G
Cuts at: 0 1169 1437
Size: 1169 268
BtsI GCAGTG_nn'
Cuts at: 0 991 1437
Size: 991 446
DrdI GACnn_nn'nnGTC
Cuts at: 0 345 1437
Size: 345 1092
EaeI y'GGCC_r
Cuts at: 0 68 649 1437
Size: 68 581 788
Fragments arranged by size:
788 581 68
EarI CTCTTCn'nnn_
Cuts at: 0 109 516 715 1055 1066 1437
Size: 109 407 199 340 11 371
Fragments arranged by size:
407 371 340 199 109 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 306 1437
Size: 306 1131
EcoICRI GAG'CTC
Cuts at: 0 102 1024 1284 1437
Size: 102 922 260 153
Fragments arranged by size:
922 260 153 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 175 244 371 790 817 822 985
Size: 49 126 69 127 419 27 5 163
Cuts at: 985 1036 1201 1282 1423 1437
Size: 51 165 81 141 14
Fragments arranged by size:
419 165 163 141 127 126 81 69
51 49 27 14 5
EcoO109I rG'GnC_Cy
Cuts at: 0 12 200 359 1185 1437
Size: 12 188 159 826 252
Fragments arranged by size:
826 252 188 159 12
EcoRI G'AATT_C
Cuts at: 0 772 1437
Size: 772 665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 453 1437
Size: 453 984
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 204 236 681 713 1437
Size: 204 32 445 32 724
Fragments arranged by size:
724 445 204 32 32
HincII GTy'rAC
Cuts at: 0 961 1437
Size: 961 476
KpnI G_GTAC'C
Cuts at: 0 948 1437
Size: 948 489
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 1154 1437
Size: 608 546 283
Fragments arranged by size:
608 546 283
MscI TGG'CCA
Cuts at: 0 70 1437
Size: 70 1367
MslI CAynn'nnrTG
Cuts at: 0 1157 1168 1437
Size: 1157 11 269
Fragments arranged by size:
1157 269 11
MspA1I CmG'CkG
Cuts at: 0 858 972 1437
Size: 858 114 465
Fragments arranged by size:
858 465 114
NcoI C'CATG_G
Cuts at: 0 1169 1437
Size: 1169 268
NdeI CA'TA_TG
Cuts at: 0 343 1437
Size: 343 1094
NspI r_CATG'y
Cuts at: 0 645 1437
Size: 645 792
PasI CC'CwG_GG
Cuts at: 0 95 1437
Size: 95 1342
PciI A'CATG_T
Cuts at: 0 641 1437
Size: 641 796
PflMI CCAn_nnn'nTGG
Cuts at: 0 66 627 1437
Size: 66 561 810
Fragments arranged by size:
810 561 66
PfoI T'CCnGG_A
Cuts at: 0 26 1437
Size: 26 1411
PpuMI rG'GwC_Cy
Cuts at: 0 200 1185 1437
Size: 200 985 252
Fragments arranged by size:
985 252 200
PshAI GACnn'nnGTC
Cuts at: 0 1265 1437
Size: 1265 172
PvuII CAG'CTG
Cuts at: 0 858 972 1437
Size: 858 114 465
Fragments arranged by size:
858 465 114
SacI G_AGCT'C
Cuts at: 0 104 1026 1286 1437
Size: 104 922 260 151
Fragments arranged by size:
922 260 151 104
SanDI GG'GwC_CC
Cuts at: 0 200 1437
Size: 200 1237
SapI GCTCTTCn'nnn_
Cuts at: 0 109 1437
Size: 109 1328
SfcI C'TryA_G
Cuts at: 0 7 1437
Size: 7 1430
SmlI C'TyrA_G
Cuts at: 0 463 1009 1437
Size: 463 546 428
Fragments arranged by size:
546 463 428
StuI AGG'CCT
Cuts at: 0 64 127 172 1431 1437
Size: 64 63 45 1259 6
Fragments arranged by size:
1259 64 63 45 6
StyI C'CwwG_G
Cuts at: 0 65 415 1169 1437
Size: 65 350 754 268
Fragments arranged by size:
754 350 268 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 494 1437
Size: 494 943
TatI w'GTAC_w
Cuts at: 0 531 544 1437
Size: 531 13 893
Fragments arranged by size:
893 531 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 8 40 1437
Size: 8 32 1397
Fragments arranged by size:
1397 32 8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 78 1398 1437
Size: 78 1320 39
Fragments arranged by size:
1320 78 39
XmnI GAAnn'nnTTC
Cuts at: 0 445 525 1437
Size: 445 80 912
Fragments arranged by size:
912 445 80
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlwNI ApaLI ApoI AvaI
BaeI BanI BanII BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BstAPI BstYI BstZ17I Bsu36I BtgI BtsI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI Hin4I HincII KpnI MmeI MscI MslI MspA1I
NcoI NdeI NspI PasI PciI PflMI PfoI PpuMI
PshAI PvuII SacI SanDI SapI SfcI SmlI StuI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BamHI
BbeI BbvCI BcgI BfrBI BmrI BmtI BplI BsaI
BsaAI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BtgZI ClaI
CspCI DraI DraIII EagI EcoNI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmaI ZraI