Restriction map for
pF1KB7426
(Linear) (Six-base) MAPSORT of: KIBB7426.seq Check: 9681 from: 1 to: 1236
KIBB7426.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AfeI AGC'GCT
Cuts at: 0 406 1215 1236
Size: 406 809 21
Fragments arranged by size:
809 406 21
AlwNI CAG_nnn'CTG
Cuts at: 0 11 1236
Size: 11 1225
ApaI G_GGCC'C
Cuts at: 0 665 1236
Size: 665 571
AvaI C'yCGr_G
Cuts at: 0 31 265 564 664 858 1236
Size: 31 234 299 100 194 378
Fragments arranged by size:
378 299 234 194 100 31
BanI G'GyrC_C
Cuts at: 0 114 455 577 636 1236
Size: 114 341 122 59 600
Fragments arranged by size:
600 341 122 114 59
BanII G_rGCy'C
Cuts at: 0 665 715 806 1236
Size: 665 50 91 430
Fragments arranged by size:
665 430 91 50
BbeI G_GCGC'C
Cuts at: 0 459 640 1236
Size: 459 181 596
Fragments arranged by size:
596 459 181
BbsI GAAGACnn'nnnn_
Cuts at: 0 1231 1236
Size: 1231 5
BbvCI CC'TCA_GC
Cuts at: 0 787 1236
Size: 787 449
BglI GCCn_nnn'nGGC
Cuts at: 0 54 575 624 660 1236
Size: 54 521 49 36 576
Fragments arranged by size:
576 521 54 49 36
BlpI GC'TnA_GC
Cuts at: 0 394 1236
Size: 394 842
Bme1580I G_kGCm'C
Cuts at: 0 665 1236
Size: 665 571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1202 1236
Size: 1202 34
Bpu10I CC'TnA_GC
Cuts at: 0 787 1236
Size: 787 449
BsaI GGTCTCn'nnnn_
Cuts at: 0 661 1236
Size: 661 575
BsaAI yAC'GTr
Cuts at: 0 348 1236
Size: 348 888
BsaBI GATnn'nnATC
Cuts at: 0 184 1236
Size: 184 1052
BsaHI Gr'CG_yC
Cuts at: 0 456 533 637 1236
Size: 456 77 104 599
Fragments arranged by size:
599 456 104 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 67 1178 1208 1236
Size: 37 30 1111 30 28
Fragments arranged by size:
1111 37 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 585 1199 1236
Size: 585 614 37
Fragments arranged by size:
614 585 37
BseYI C'CCAG_C
Cuts at: 0 723 1236
Size: 723 513
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 364 1236
Size: 345 19 872
Fragments arranged by size:
872 345 19
BsiEI CG_ry'CG
Cuts at: 0 709 838 854 1236
Size: 709 129 16 382
Fragments arranged by size:
709 382 129 16
BsiHKAI G_wGCw'C
Cuts at: 0 485 1236
Size: 485 751
BsmI GAATG_Cn'
Cuts at: 0 706 1236
Size: 706 530
Bsp1286I G_dGCh'C
Cuts at: 0 485 665 715 806 1236
Size: 485 180 50 91 430
Fragments arranged by size:
485 430 180 91 50
BsrBI CCG'CTC
Cuts at: 0 161 429 1090 1236
Size: 161 268 661 146
Fragments arranged by size:
661 268 161 146
BsrFI r'CCGG_y
Cuts at: 0 117 508 704 830 878 1236
Size: 117 391 196 126 48 358
Fragments arranged by size:
391 358 196 126 117 48
BssHII G'CGCG_C
Cuts at: 0 467 728 1236
Size: 467 261 508
Fragments arranged by size:
508 467 261
BstEII G'GTnAC_C
Cuts at: 0 120 171 447 750 1236
Size: 120 51 276 303 486
Fragments arranged by size:
486 303 276 120 51
BtgI C'CryG_G
Cuts at: 0 52 491 671 680 1236
Size: 52 439 180 9 556
Fragments arranged by size:
556 439 180 52 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 459 1236
Size: 459 777
BtsI GCAGTG_nn'
Cuts at: 0 1154 1236
Size: 1154 82
DraI TTT'AAA
Cuts at: 0 1101 1236
Size: 1101 135
EaeI y'GGCC_r
Cuts at: 0 239 413 706 835 851 1236
Size: 239 174 293 129 16 385
Fragments arranged by size:
385 293 239 174 129 16
EagI C'GGCC_G
Cuts at: 0 706 835 851 1236
Size: 706 129 16 385
Fragments arranged by size:
706 385 129 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1202 1236
Size: 1202 34
EcoO109I rG'GnC_Cy
Cuts at: 0 543 661 1236
Size: 543 118 575
Fragments arranged by size:
575 543 118
HaeII r_GCGC'y
Cuts at: 0 84 112 195 300 408 459 499 640
Size: 84 28 83 105 108 51 40 141
Cuts at: 640 688 1217 1236
Size: 48 529 19
Fragments arranged by size:
529 141 108 105 84 83 51 48
40 28 19
KasI G'GCGC_C
Cuts at: 0 455 636 1236
Size: 455 181 600
Fragments arranged by size:
600 455 181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 1236
Size: 272 964
MscI TGG'CCA
Cuts at: 0 415 1236
Size: 415 821
MslI CAynn'nnrTG
Cuts at: 0 241 478 1236
Size: 241 237 758
Fragments arranged by size:
758 241 237
MspA1I CmG'CkG
Cuts at: 0 86 127 324 673 682 723 840 1236
Size: 86 41 197 349 9 41 117 396
Fragments arranged by size:
396 349 197 117 86 41 41 9
NaeI GCC'GGC
Cuts at: 0 706 832 1236
Size: 706 126 404
Fragments arranged by size:
706 404 126
NarI GG'CG_CC
Cuts at: 0 456 637 1236
Size: 456 181 599
Fragments arranged by size:
599 456 181
NgoMIV G'CCGG_C
Cuts at: 0 704 830 1236
Size: 704 126 406
Fragments arranged by size:
704 406 126
NotI GC'GGCC_GC
Cuts at: 0 835 1236
Size: 835 401
NspI r_CATG'y
Cuts at: 0 207 1236
Size: 207 1029
PasI CC'CwG_GG
Cuts at: 0 102 308 1236
Size: 102 206 928
Fragments arranged by size:
928 206 102
PpuMI rG'GwC_Cy
Cuts at: 0 543 1236
Size: 543 693
PsiI TTA'TAA
Cuts at: 0 922 1236
Size: 922 314
PspOMI G'GGCC_C
Cuts at: 0 661 1236
Size: 661 575
PvuII CAG'CTG
Cuts at: 0 723 1236
Size: 723 513
SacII CC_GC'GG
Cuts at: 0 674 683 1236
Size: 674 9 553
Fragments arranged by size:
674 553 9
ScaI AGT'ACT
Cuts at: 0 1003 1236
Size: 1003 233
SfoI GGC'GCC
Cuts at: 0 457 638 1236
Size: 457 181 598
Fragments arranged by size:
598 457 181
SmaI CCC'GGG
Cuts at: 0 566 1236
Size: 566 670
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 300 1236
Size: 300 936
TatI w'GTAC_w
Cuts at: 0 989 1001 1082 1236
Size: 989 12 81 154
Fragments arranged by size:
989 154 81 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 1178 1210 1236
Size: 37 32 1109 32 26
Fragments arranged by size:
1109 37 32 32 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 225 1236
Size: 225 1011
XmaI C'CCGG_G
Cuts at: 0 564 1236
Size: 564 672
Enzymes that do cut:
AfeI AlwNI ApaI AvaI BanI BanII BbeI BbsI
BbvCI BglI BlpI Bme1580I BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BsrBI BsrFI BssHII BstEII BtgI BtgZI
BtsI DraI EaeI EagI Eco57MI EcoO109I HaeII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PasI PpuMI PsiI PspOMI PvuII SacII ScaI
SfoI SmaI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI BplI BpuEI BsaWI
BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I ClaI CspCI
DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI NcoI NdeI NheI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PspXI PsrI PstI PvuI RsrII SacI SalI SanDI
SapI SbfI SexAI SfcI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
Tth111I XbaI XhoI XmnI ZraI