(Linear) (Six-base) MAPSORT of: KIBB7427.seq  Check: 8484  from: 1  to: 456



KIBB7427.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

Acc65I G'GTAC_C

Cuts at:      0     271     456 
   Size:        271     185
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     456 
   Size:        403      53
 
ApoI r'AATT_y

Cuts at:      0     226     456 
   Size:        226     230
 
AseI AT'TA_AT

Cuts at:      0     340     456 
   Size:        340     116
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     255     288     456 
   Size:        255      33     168

  Fragments arranged by size: 

                 255     168      33
 
BanI G'GyrC_C

Cuts at:      0     271     456 
   Size:        271     185
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     170     456 
   Size:        170     286
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     272     456 
   Size:        272     184
 
BstBI TT'CG_AA

Cuts at:      0     105     456 
   Size:        105     351
 
BstEII G'GTnAC_C

Cuts at:      0      26     456 
   Size:         26     430
 
EaeI y'GGCC_r

Cuts at:      0     356     456 
   Size:        356     100
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     456 
   Size:        403      53
 
EcoRI G'AATT_C

Cuts at:      0     226     456 
   Size:        226     230
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     269     271     301     303     456 
   Size:        269       2      30       2     153

  Fragments arranged by size: 

                 269     153      30       2       2
 
KpnI G_GTAC'C

Cuts at:      0     275     456 
   Size:        275     181
 
MscI TGG'CCA

Cuts at:      0     358     456 
   Size:        358      98
 
MslI CAynn'nnrTG

Cuts at:      0     315     456 
   Size:        315     141
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     296     328     456 
   Size:        296      32     128

  Fragments arranged by size: 

                 296     128      32
 
PsiI TTA'TAA

Cuts at:      0     398     456 
   Size:        398      58
 
SfcI C'TryA_G

Cuts at:      0      21     456 
   Size:         21     435
 
SspI AAT'ATT

Cuts at:      0     110     297     456 
   Size:        110     187     159

  Fragments arranged by size: 

                 187     159     110
 
StyI C'CwwG_G

Cuts at:      0     359     456 
   Size:        359      97
 
Tth111I GACn'n_nGTC

Cuts at:      0      51     456 
   Size:         51     405
 
XbaI T'CTAG_A

Cuts at:      0      93     456 
   Size:         93     363
 

 Enzymes that do cut:

   Acc65I     AcuI     ApoI     AseI     BaeI     BanI     BbsI     BsaI
    BstBI   BstEII     EaeI  Eco57MI    EcoRI    Hin4I     KpnI     MscI
     MslI     PpiI     PsiI     SfcI     SspI     StyI  Tth111I     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII    BamHI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TatI     TstI     XcmI
     XhoI     XmaI     XmnI     ZraI