(Linear) (Six-base) MAPSORT of: KIBB7430.seq  Check: 3787  from: 1  to: 1488



KIBB7430.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1169    1488 
   Size:       1169     319
 
AccI GT'mk_AC

Cuts at:      0     755    1488 
   Size:        755     733
 
AfeI AGC'GCT

Cuts at:      0     324    1488 
   Size:        324    1164
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1358    1488 
   Size:       1358     130
 
ApoI r'AATT_y

Cuts at:      0     691    1327    1488 
   Size:        691     636     161

  Fragments arranged by size: 

                 691     636     161
 
BanII G_rGCy'C

Cuts at:      0      57    1197    1488 
   Size:         57    1140     291

  Fragments arranged by size: 

                1140     291      57
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     992    1488 
   Size:        992     496
 
BbvCI CC'TCA_GC

Cuts at:      0    1480    1488 
   Size:       1480       8
 
BglI GCCn_nnn'nGGC

Cuts at:      0     512    1488 
   Size:        512     976
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     441    1488 
   Size:        441    1047
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1264    1488 
   Size:       1264     224
 
Bpu10I CC'TnA_GC

Cuts at:      0     566     631    1480    1488 
   Size:        566      65     849       8

  Fragments arranged by size: 

                 849     566      65       8
 
BsaWI w'CCGG_w

Cuts at:      0      21    1488 
   Size:         21    1467
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     156     186    1488 
   Size:        156      30    1302

  Fragments arranged by size: 

                1302     156      30
 
BsiEI CG_ry'CG

Cuts at:      0      36    1137    1488 
   Size:         36    1101     351

  Fragments arranged by size: 

                1101     351      36
 
Bsp1286I G_dGCh'C

Cuts at:      0      57    1197    1488 
   Size:         57    1140     291

  Fragments arranged by size: 

                1140     291      57
 
BspEI T'CCGG_A

Cuts at:      0      21    1488 
   Size:         21    1467
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     108    1008    1169    1488 
   Size:        108     900     161     319

  Fragments arranged by size: 

                 900     319     161     108
 
BsrFI r'CCGG_y

Cuts at:      0      70    1488 
   Size:         70    1418
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     521    1488 
   Size:        521     967
 
BtsI GCAGTG_nn'

Cuts at:      0     485    1488 
   Size:        485    1003
 
ClaI AT'CG_AT

Cuts at:      0    1133    1488 
   Size:       1133     355
 
EaeI y'GGCC_r

Cuts at:      0      33     451     504    1488 
   Size:         33     418      53     984

  Fragments arranged by size: 

                 984     418      53      33
 
EagI C'GGCC_G

Cuts at:      0      33    1488 
   Size:         33    1455
 
EarI CTCTTCn'nnn_

Cuts at:      0     293     432     524     732    1406    1488 
   Size:        293     139      92     208     674      82

  Fragments arranged by size: 

                 674     293     208     139      92      82
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      65    1488 
   Size:         65    1423
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1264    1488 
   Size:       1264     224
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     926    1488 
   Size:        926     562
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     732    1123    1488 
   Size:        732     391     365

  Fragments arranged by size: 

                 732     391     365
 
HaeII r_GCGC'y

Cuts at:      0      12     326    1488 
   Size:         12     314    1162

  Fragments arranged by size: 

                1162     314      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      70     102    1488 
   Size:         70      32    1386

  Fragments arranged by size: 

                1386      70      32
 
HincII GTy'rAC

Cuts at:      0     844    1468    1488 
   Size:        844     624      20

  Fragments arranged by size: 

                 844     624      20
 
HindIII A'AGCT_T

Cuts at:      0    1339    1488 
   Size:       1339     149
 
MfeI C'AATT_G

Cuts at:      0     593    1488 
   Size:        593     895
 
MscI TGG'CCA

Cuts at:      0     506    1488 
   Size:        506     982
 
MslI CAynn'nnrTG

Cuts at:      0      90    1163    1488 
   Size:         90    1073     325

  Fragments arranged by size: 

                1073     325      90
 
MspA1I CmG'CkG

Cuts at:      0      32     909    1488 
   Size:         32     877     579

  Fragments arranged by size: 

                 877     579      32
 
NaeI GCC'GGC

Cuts at:      0      72    1488 
   Size:         72    1416
 
NgoMIV G'CCGG_C

Cuts at:      0      70    1488 
   Size:         70    1418
 
NspI r_CATG'y

Cuts at:      0    1204    1488 
   Size:       1204     284
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     845     877    1488 
   Size:        845      32     611

  Fragments arranged by size: 

                 845     611      32
 
PvuI CG_AT'CG

Cuts at:      0    1137    1488 
   Size:       1137     351
 
PvuII CAG'CTG

Cuts at:      0     909    1488 
   Size:        909     579
 
SfcI C'TryA_G

Cuts at:      0     702     752    1488 
   Size:        702      50     736

  Fragments arranged by size: 

                 736     702      50
 
SspI AAT'ATT

Cuts at:      0     829    1488 
   Size:        829     659
 
StyI C'CwwG_G

Cuts at:      0     964    1488 
   Size:        964     524
 
TatI w'GTAC_w

Cuts at:      0     376    1488 
   Size:        376    1112
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     117     149    1488 
   Size:        117      32    1339

  Fragments arranged by size: 

                1339     117      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     461    1488 
   Size:        461    1027
 
XmnI GAAnn'nnTTC

Cuts at:      0     445     989    1106    1488 
   Size:        445     544     117     382

  Fragments arranged by size: 

                 544     445     382     117
 

 Enzymes that do cut:

     AarI     AccI     AfeI     AlfI     ApoI    BanII     BbsI    BbvCI
     BglI     BplI     BpmI   Bpu10I    BsaWI    BsaXI    BsiEI Bsp1286I
    BspEI    BspMI    BsrFI   BstAPI     BtsI     ClaI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI     FalI    HaeII    Hin4I   HincII
  HindIII     MfeI     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI
     PsrI     PvuI    PvuII     SfcI     SspI     StyI     TatI     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI    CspCI     DraI   DraIII     DrdI  EcoICRI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI     HpaI     KasI     KpnI
     MluI     MmeI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI
     XmaI     ZraI