Restriction map for
pF1KB7431
(Linear) (Six-base) MAPSORT of: KIBB7431.seq Check: 2182 from: 1 to: 924
KIBB7431.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AatII G_ACGT'C
Cuts at: 0 901 924
Size: 901 23
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 902 924
Size: 102 800 22
Fragments arranged by size:
800 102 22
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 868 924
Size: 868 56
AlwNI CAG_nnn'CTG
Cuts at: 0 319 924
Size: 319 605
ApaI G_GGCC'C
Cuts at: 0 305 924
Size: 305 619
BanI G'GyrC_C
Cuts at: 0 30 109 445 924
Size: 30 79 336 479
Fragments arranged by size:
479 336 79 30
BanII G_rGCy'C
Cuts at: 0 16 305 924
Size: 16 289 619
Fragments arranged by size:
619 289 16
BbeI G_GCGC'C
Cuts at: 0 449 924
Size: 449 475
BbsI GAAGACnn'nnnn_
Cuts at: 0 539 924
Size: 539 385
BciVI GTATCCnnnnn_n'
Cuts at: 0 722 924
Size: 722 202
BclI T'GATC_A
Cuts at: 0 515 924
Size: 515 409
BglI GCCn_nnn'nGGC
Cuts at: 0 11 39 274 382 924
Size: 11 28 235 108 542
Fragments arranged by size:
542 235 108 28 11
BlpI GC'TnA_GC
Cuts at: 0 541 924
Size: 541 383
Bme1580I G_kGCm'C
Cuts at: 0 112 279 305 924
Size: 112 167 26 619
Fragments arranged by size:
619 167 112 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 906 924
Size: 906 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 376 580 924
Size: 346 30 204 344
Fragments arranged by size:
346 344 204 30
BsaHI Gr'CG_yC
Cuts at: 0 446 898 924
Size: 446 452 26
Fragments arranged by size:
452 446 26
BsaWI w'CCGG_w
Cuts at: 0 714 924
Size: 714 210
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 250 253 277 343 924
Size: 196 54 3 24 66 581
Fragments arranged by size:
581 196 66 54 24 3
BseYI C'CCAG_C
Cuts at: 0 269 924
Size: 269 655
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 305 924
Size: 154 151 619
Fragments arranged by size:
619 154 151
BsiEI CG_ry'CG
Cuts at: 0 149 924
Size: 149 775
BsmBI CGTCTCn'nnnn_
Cuts at: 0 889 924
Size: 889 35
Bsp1286I G_dGCh'C
Cuts at: 0 16 112 279 305 924
Size: 16 96 167 26 619
Fragments arranged by size:
619 167 96 26 16
BspEI T'CCGG_A
Cuts at: 0 714 924
Size: 714 210
BsrBI CCG'CTC
Cuts at: 0 255 414 924
Size: 255 159 510
Fragments arranged by size:
510 255 159
BsrFI r'CCGG_y
Cuts at: 0 360 924
Size: 360 564
BssSI C'ACGA_G
Cuts at: 0 247 924
Size: 247 677
BstAPI GCAn_nnn'nTGC
Cuts at: 0 288 924
Size: 288 636
BstXI CCAn_nnnn'nTGG
Cuts at: 0 707 924
Size: 707 217
BstYI r'GATC_y
Cuts at: 0 802 821 924
Size: 802 19 103
Fragments arranged by size:
802 103 19
Bsu36I CC'TnA_GG
Cuts at: 0 8 924
Size: 8 916
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 684 719 924
Size: 684 35 205
Fragments arranged by size:
684 205 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 315 924
Size: 315 609
EaeI y'GGCC_r
Cuts at: 0 146 338 924
Size: 146 192 586
Fragments arranged by size:
586 192 146
EagI C'GGCC_G
Cuts at: 0 146 924
Size: 146 778
EarI CTCTTCn'nnn_
Cuts at: 0 566 878 924
Size: 566 312 46
Fragments arranged by size:
566 312 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 229 247 924
Size: 229 18 677
Fragments arranged by size:
677 229 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 346 376 580 902 924
Size: 102 244 30 204 322 22
Fragments arranged by size:
322 244 204 102 30 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 829 924
Size: 829 95
EcoO109I rG'GnC_Cy
Cuts at: 0 243 834 924
Size: 243 591 90
Fragments arranged by size:
591 243 90
HaeII r_GCGC'y
Cuts at: 0 449 924
Size: 449 475
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 434 466 924
Size: 434 32 458
Fragments arranged by size:
458 434 32
KasI G'GCGC_C
Cuts at: 0 445 924
Size: 445 479
MfeI C'AATT_G
Cuts at: 0 701 924
Size: 701 223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 924
Size: 179 745
MspA1I CmG'CkG
Cuts at: 0 273 399 924
Size: 273 126 525
Fragments arranged by size:
525 273 126
NarI GG'CG_CC
Cuts at: 0 446 924
Size: 446 478
NspI r_CATG'y
Cuts at: 0 540 924
Size: 540 384
PasI CC'CwG_GG
Cuts at: 0 298 924
Size: 298 626
PpuMI rG'GwC_Cy
Cuts at: 0 834 924
Size: 834 90
PspOMI G'GGCC_C
Cuts at: 0 301 924
Size: 301 623
SfoI GGC'GCC
Cuts at: 0 447 924
Size: 447 477
StuI AGG'CCT
Cuts at: 0 82 385 733 924
Size: 82 303 348 191
Fragments arranged by size:
348 303 191 82
StyI C'CwwG_G
Cuts at: 0 83 101 650 924
Size: 83 18 549 274
Fragments arranged by size:
549 274 83 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 463 657 924
Size: 463 194 267
Fragments arranged by size:
463 267 194
ZraI GAC'GTC
Cuts at: 0 899 924
Size: 899 25
Enzymes that do cut:
AatII AcuI AlfI AlwNI ApaI BanI BanII BbeI
BbsI BciVI BclI BglI BlpI Bme1580I BmrI BpmI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I
BspEI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I
CspCI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MfeI MmeI MspA1I NarI
NspI PasI PpuMI PspOMI SfoI StuI StyI XcmI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BfrBI BglII
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaXI BsiHKAI BsiWI BsmI BspHI BspMI BsrDI BsrGI
BssHII BstBI BstEII BstZ17I BtgI BtgZI BtsI ClaI
DraI DraIII EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MluI MscI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI