Restriction map for
pF1KB7432
(Linear) (Six-base) MAPSORT of: KIBB7432.seq Check: 2119 from: 1 to: 984
KIBB7432.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AccI GT'mk_AC
Cuts at: 0 107 984
Size: 107 877
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 677 984
Size: 99 578 307
Fragments arranged by size:
578 307 99
AleI CACnn'nnGTG
Cuts at: 0 483 844 984
Size: 483 361 140
Fragments arranged by size:
483 361 140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 409 984
Size: 409 575
ApoI r'AATT_y
Cuts at: 0 77 984
Size: 77 907
AseI AT'TA_AT
Cuts at: 0 340 984
Size: 340 644
BanI G'GyrC_C
Cuts at: 0 704 763 984
Size: 704 59 221
Fragments arranged by size:
704 221 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 708 984
Size: 708 276
BciVI GTATCCnnnnn_n'
Cuts at: 0 968 984
Size: 968 16
BclI T'GATC_A
Cuts at: 0 146 984
Size: 146 838
BfrBI ATG'CAT
Cuts at: 0 454 661 984
Size: 454 207 323
Fragments arranged by size:
454 323 207
Bme1580I G_kGCm'C
Cuts at: 0 707 984
Size: 707 277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 567 984
Size: 567 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 789 984
Size: 789 195
BsaBI GATnn'nnATC
Cuts at: 0 145 984
Size: 145 839
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 984
Size: 187 30 767
Fragments arranged by size:
767 187 30
BsiHKAI G_wGCw'C
Cuts at: 0 490 984
Size: 490 494
BsmI GAATG_Cn'
Cuts at: 0 661 984
Size: 661 323
Bsp1286I G_dGCh'C
Cuts at: 0 490 707 984
Size: 490 217 277
Fragments arranged by size:
490 277 217
BsrDI GCAATG_nn'
Cuts at: 0 233 984
Size: 233 751
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 438 984
Size: 249 189 546
Fragments arranged by size:
546 249 189
BtgI C'CryG_G
Cuts at: 0 759 842 984
Size: 759 83 142
Fragments arranged by size:
759 142 83
DraI TTT'AAA
Cuts at: 0 497 984
Size: 497 487
DraIII CAC_nnn'GTG
Cuts at: 0 375 984
Size: 375 609
EaeI y'GGCC_r
Cuts at: 0 377 984
Size: 377 607
EarI CTCTTCn'nnn_
Cuts at: 0 91 778 984
Size: 91 687 206
Fragments arranged by size:
687 206 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 677 789 984
Size: 99 578 112 195
Fragments arranged by size:
578 195 112 99
EcoRV GAT'ATC
Cuts at: 0 560 984
Size: 560 424
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 861 893 984
Size: 861 32 91
Fragments arranged by size:
861 91 32
MscI TGG'CCA
Cuts at: 0 379 984
Size: 379 605
MslI CAynn'nnrTG
Cuts at: 0 436 483 844 984
Size: 436 47 361 140
Fragments arranged by size:
436 361 140 47
NcoI C'CATG_G
Cuts at: 0 759 984
Size: 759 225
NsiI A_TGCA'T
Cuts at: 0 456 663 984
Size: 456 207 321
Fragments arranged by size:
456 321 207
NspI r_CATG'y
Cuts at: 0 470 984
Size: 470 514
PflMI CCAn_nnn'nTGG
Cuts at: 0 375 984
Size: 375 609
SexAI A'CCwGG_T
Cuts at: 0 257 984
Size: 257 727
SfcI C'TryA_G
Cuts at: 0 873 984
Size: 873 111
SspI AAT'ATT
Cuts at: 0 931 984
Size: 931 53
StyI C'CwwG_G
Cuts at: 0 759 984
Size: 759 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 284 316 984
Size: 284 32 668
Fragments arranged by size:
668 284 32
XmnI GAAnn'nnTTC
Cuts at: 0 279 341 984
Size: 279 62 643
Fragments arranged by size:
643 279 62
Enzymes that do cut:
AccI AcuI AleI AlfI ApoI AseI BanI BbsI
BciVI BclI BfrBI Bme1580I BplI BpmI BsaBI BsaXI
BsiHKAI BsmI Bsp1286I BsrDI BstXI BtgI DraI DraIII
EaeI EarI Eco57MI EcoRV Hin4I MscI MslI NcoI
NsiI NspI PflMI SexAI SfcI SspI StyI TstI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BglI
BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmaI ZraI