Restriction map for
pF1KB7433
(Linear) (Six-base) MAPSORT of: KIBB7433.seq Check: 9687 from: 1 to: 957
KIBB7433.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 922 957
Size: 713 209 35
Fragments arranged by size:
713 209 35
AflIII A'CryG_T
Cuts at: 0 653 957
Size: 653 304
ApoI r'AATT_y
Cuts at: 0 249 957
Size: 249 708
BanI G'GyrC_C
Cuts at: 0 757 957
Size: 757 200
BanII G_rGCy'C
Cuts at: 0 688 957
Size: 688 269
BbsI GAAGACnn'nnnn_
Cuts at: 0 705 819 957
Size: 705 114 138
Fragments arranged by size:
705 138 114
BciVI GTATCCnnnnn_n'
Cuts at: 0 147 957
Size: 147 810
BfrBI ATG'CAT
Cuts at: 0 286 957
Size: 286 671
BlpI GC'TnA_GC
Cuts at: 0 687 957
Size: 687 270
BmrI ACTGGGnnnn_n'
Cuts at: 0 310 957
Size: 310 647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 957
Size: 395 562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 774 957
Size: 774 183
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 541 957
Size: 541 416
BsiHKAI G_wGCw'C
Cuts at: 0 688 957
Size: 688 269
BsmI GAATG_Cn'
Cuts at: 0 422 957
Size: 422 535
Bsp1286I G_dGCh'C
Cuts at: 0 688 957
Size: 688 269
BsrBI CCG'CTC
Cuts at: 0 151 957
Size: 151 806
BsrGI T'GTAC_A
Cuts at: 0 257 957
Size: 257 700
Bsu36I CC'TnA_GG
Cuts at: 0 553 676 957
Size: 553 123 281
Fragments arranged by size:
553 281 123
BtgI C'CryG_G
Cuts at: 0 350 957
Size: 350 607
EaeI y'GGCC_r
Cuts at: 0 371 957
Size: 371 586
EarI CTCTTCn'nnn_
Cuts at: 0 112 225 957
Size: 112 113 732
Fragments arranged by size:
732 113 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 481 957
Size: 481 476
EcoICRI GAG'CTC
Cuts at: 0 686 957
Size: 686 271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 713 922 957
Size: 395 318 209 35
Fragments arranged by size:
395 318 209 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 855 887 957
Size: 855 32 70
Fragments arranged by size:
855 70 32
HincII GTy'rAC
Cuts at: 0 522 957
Size: 522 435
MfeI C'AATT_G
Cuts at: 0 12 957
Size: 12 945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 957
Size: 536 421
MscI TGG'CCA
Cuts at: 0 373 957
Size: 373 584
MslI CAynn'nnrTG
Cuts at: 0 172 319 957
Size: 172 147 638
Fragments arranged by size:
638 172 147
MspA1I CmG'CkG
Cuts at: 0 691 957
Size: 691 266
NcoI C'CATG_G
Cuts at: 0 350 957
Size: 350 607
NsiI A_TGCA'T
Cuts at: 0 288 957
Size: 288 669
NspI r_CATG'y
Cuts at: 0 290 546 657 735 957
Size: 290 256 111 78 222
Fragments arranged by size:
290 256 222 111 78
PciI A'CATG_T
Cuts at: 0 653 957
Size: 653 304
PfoI T'CCnGG_A
Cuts at: 0 411 957
Size: 411 546
PvuII CAG'CTG
Cuts at: 0 691 957
Size: 691 266
SacI G_AGCT'C
Cuts at: 0 688 957
Size: 688 269
SfcI C'TryA_G
Cuts at: 0 450 720 957
Size: 450 270 237
Fragments arranged by size:
450 270 237
SmlI C'TyrA_G
Cuts at: 0 789 957
Size: 789 168
StyI C'CwwG_G
Cuts at: 0 350 957
Size: 350 607
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 34 249 377 957
Size: 34 215 128 580
Fragments arranged by size:
580 215 128 34
TatI w'GTAC_w
Cuts at: 0 176 257 773 957
Size: 176 81 516 184
Fragments arranged by size:
516 184 176 81
Enzymes that do cut:
AcuI AflIII ApoI BanI BanII BbsI BciVI BfrBI
BlpI BmrI BpmI BpuEI BseRI BsiHKAI BsmI Bsp1286I
BsrBI BsrGI Bsu36I BtgI EaeI EarI EciI EcoICRI
Eco57MI Hin4I HincII MfeI MmeI MscI MslI MspA1I
NcoI NsiI NspI PciI PfoI PvuII SacI SfcI
SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BcgI BclI BglI BglII Bme1580I BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI