(Linear) (Six-base) MAPSORT of: KIBB7434.seq  Check: 5134  from: 1  to: 564



KIBB7434.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AvaI C'yCGr_G

Cuts at:      0     423     564 
   Size:        423     141
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       1      35      64      98     315     349     564 
   Size:          1      34      29      34     217      34     215

  Fragments arranged by size: 

                 217     215      34      34      34      29       1
 
Bme1580I G_kGCm'C

Cuts at:      0     421     564 
   Size:        421     143
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     564 
   Size:        295     269
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      21     564 
   Size:         21     543
 
BsaHI Gr'CG_yC

Cuts at:      0      14     564 
   Size:         14     550
 
BsiEI CG_ry'CG

Cuts at:      0     261     564 
   Size:        261     303
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       2     564 
   Size:          2     562
 
Bsp1286I G_dGCh'C

Cuts at:      0     421     564 
   Size:        421     143
 
BspHI T'CATG_A

Cuts at:      0     170     564 
   Size:        170     394
 
BstEII G'GTnAC_C

Cuts at:      0     192     564 
   Size:        192     372
 
Bsu36I CC'TnA_GG

Cuts at:      0     548     564 
   Size:        548      16
 
BtgI C'CryG_G

Cuts at:      0     237     564 
   Size:        237     327
 
EarI CTCTTCn'nnn_

Cuts at:      0     527     564 
   Size:        527      37
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     564 
   Size:        295     269
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      21     564 
   Size:         21     543
 
MslI CAynn'nnrTG

Cuts at:      0     242     564 
   Size:        242     322
 
MspA1I CmG'CkG

Cuts at:      0     414     519     564 
   Size:        414     105      45

  Fragments arranged by size: 

                 414     105      45
 
NspI r_CATG'y

Cuts at:      0     487     564 
   Size:        487      77
 
PasI CC'CwG_GG

Cuts at:      0      32     564 
   Size:         32     532
 
PstI C_TGCA'G

Cuts at:      0     231     564 
   Size:        231     333
 
PvuI CG_AT'CG

Cuts at:      0     261     564 
   Size:        261     303
 
PvuII CAG'CTG

Cuts at:      0     414     519     564 
   Size:        414     105      45

  Fragments arranged by size: 

                 414     105      45
 
SfcI C'TryA_G

Cuts at:      0     227     564 
   Size:        227     337
 
SmaI CCC'GGG

Cuts at:      0     425     564 
   Size:        425     139
 
StuI AGG'CCT

Cuts at:      0      70     564 
   Size:         70     494
 
StyI C'CwwG_G

Cuts at:      0     440     564 
   Size:        440     124
 
XmaI C'CCGG_G

Cuts at:      0     423     564 
   Size:        423     141
 

 Enzymes that do cut:

     AvaI     BcgI Bme1580I     BpmI     BsaI    BsaHI    BsiEI    BsmBI
 Bsp1286I    BspHI   BstEII   Bsu36I     BtgI     EarI  Eco57MI    EcoNI
     MslI   MspA1I     NspI     PasI     PstI     PvuI    PvuII     SfcI
     SmaI     StuI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI