(Linear) (Six-base) MAPSORT of: KIBB7435.seq  Check: 7698  from: 1  to: 420



KIBB7435.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     420 
   Size:        121     299
 
AflIII A'CryG_T

Cuts at:      0     229     420 
   Size:        229     191
 
ApoI r'AATT_y

Cuts at:      0      74     420 
   Size:         74     346
 
BanII G_rGCy'C

Cuts at:      0      35     420 
   Size:         35     385
 
BglI GCCn_nnn'nGGC

Cuts at:      0     130     420 
   Size:        130     290
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     173     420 
   Size:        173     247
 
Bpu10I CC'TnA_GC

Cuts at:      0      37     420 
   Size:         37     383
 
BsaBI GATnn'nnATC

Cuts at:      0      86     420 
   Size:         86     334
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43     420 
   Size:         43     377
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     420 
   Size:         79     341
 
BsiHKAI G_wGCw'C

Cuts at:      0      35     420 
   Size:         35     385
 
BsmI GAATG_Cn'

Cuts at:      0     271     420 
   Size:        271     149
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     420 
   Size:         35     385
 
BsrFI r'CCGG_y

Cuts at:      0      15     420 
   Size:         15     405
 
BssHII G'CGCG_C

Cuts at:      0      68     420 
   Size:         68     352
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     305     420 
   Size:        305     115
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     205     420 
   Size:        205     215
 
DraIII CAC_nnn'GTG

Cuts at:      0     181     420 
   Size:        181     239
 
EaeI y'GGCC_r

Cuts at:      0       2     420 
   Size:          2     418
 
EarI CTCTTCn'nnn_

Cuts at:      0     283     420 
   Size:        283     137
 
EcoICRI GAG'CTC

Cuts at:      0      33     420 
   Size:         33     387
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     420 
   Size:        121     299
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     334     420 
   Size:        334      86
 
EcoO109I rG'GnC_Cy

Cuts at:      0     201     347     420 
   Size:        201     146      73

  Fragments arranged by size: 

                 201     146      73
 
EcoRI G'AATT_C

Cuts at:      0      74     420 
   Size:         74     346
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     126     333     420 
   Size:        126     207      87

  Fragments arranged by size: 

                 207     126      87
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     139     143     171     175     336     368     420 
   Size:        139       4      28       4     161      32      52

  Fragments arranged by size: 

                 161     139      52      32      28       4       4
 
HincII GTy'rAC

Cuts at:      0     383     420 
   Size:        383      37
 
MslI CAynn'nnrTG

Cuts at:      0     228     420 
   Size:        228     192
 
NaeI GCC'GGC

Cuts at:      0      17     420 
   Size:         17     403
 
NgoMIV G'CCGG_C

Cuts at:      0      15     420 
   Size:         15     405
 
NspI r_CATG'y

Cuts at:      0     233     420 
   Size:        233     187
 
PciI A'CATG_T

Cuts at:      0     229     420 
   Size:        229     191
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     135     420 
   Size:        135     285
 
PpuMI rG'GwC_Cy

Cuts at:      0     201     347     420 
   Size:        201     146      73

  Fragments arranged by size: 

                 201     146      73
 
SacI G_AGCT'C

Cuts at:      0      35     420 
   Size:         35     385
 
SexAI A'CCwGG_T

Cuts at:      0     197     420 
   Size:        197     223
 
StuI AGG'CCT

Cuts at:      0     133     420 
   Size:        133     287
 
StyI C'CwwG_G

Cuts at:      0     134     204     420 
   Size:        134      70     216

  Fragments arranged by size: 

                 216     134      70
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     142     192     420 
   Size:        142      50     228

  Fragments arranged by size: 

                 228     142      50
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     132     420 
   Size:        132     288
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI    BanII     BglI     BmrI   Bpu10I    BsaBI
    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrFI   BssHII   BstAPI
    BstXI   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    Hin4I   HincII     MslI     NaeI   NgoMIV     NspI
     PciI    PflMI    PpuMI     SacI    SexAI     StuI     StyI    TaqII
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BssSI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EagI     EciI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI