(Linear) (Six-base) MAPSORT of: KIBB7436.seq  Check: 4768  from: 1  to: 441



KIBB7436.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      96     128     441 
   Size:         96      32     313

  Fragments arranged by size: 

                 313      96      32
 
ApaLI G'TGCA_C

Cuts at:      0     286     441 
   Size:        286     155
 
ApoI r'AATT_y

Cuts at:      0     251     441 
   Size:        251     190
 
AvaI C'yCGr_G

Cuts at:      0     379     441 
   Size:        379      62
 
Bme1580I G_kGCm'C

Cuts at:      0     290     405     441 
   Size:        290     115      36

  Fragments arranged by size: 

                 290     115      36
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      20     441 
   Size:         20     421
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     201     441 
   Size:         54     147     240

  Fragments arranged by size: 

                 240     147      54
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     441 
   Size:        160     281
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      96     126     441 
   Size:         96      30     315

  Fragments arranged by size: 

                 315      96      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     290     441 
   Size:        290     151
 
Bsp1286I G_dGCh'C

Cuts at:      0     290     405     441 
   Size:        290     115      36

  Fragments arranged by size: 

                 290     115      36
 
BspHI T'CATG_A

Cuts at:      0     429     441 
   Size:        429      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     323     441 
   Size:        323     118
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      62     441 
   Size:         62     379
 
BstYI r'GATC_y

Cuts at:      0     159     441 
   Size:        159     282
 
BtgI C'CryG_G

Cuts at:      0     390     441 
   Size:        390      51
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      32     441 
   Size:         32     409
 
DraIII CAC_nnn'GTG

Cuts at:      0     220     441 
   Size:        220     221
 
EaeI y'GGCC_r

Cuts at:      0     420     441 
   Size:        420      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     201     441 
   Size:         54     147     240

  Fragments arranged by size: 

                 240     147      54
 
MscI TGG'CCA

Cuts at:      0     422     441 
   Size:        422      19
 
MslI CAynn'nnrTG

Cuts at:      0     428     441 
   Size:        428      13
 
NspI r_CATG'y

Cuts at:      0     111     441 
   Size:        111     330
 
PfoI T'CCnGG_A

Cuts at:      0      31     441 
   Size:         31     410
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      96     128     441 
   Size:         96      32     313

  Fragments arranged by size: 

                 313      96      32
 
SmlI C'TyrA_G

Cuts at:      0     139     379     441 
   Size:        139     240      62

  Fragments arranged by size: 

                 240     139      62
 
SspI AAT'ATT

Cuts at:      0      78     441 
   Size:         78     363
 
StyI C'CwwG_G

Cuts at:      0     267     441 
   Size:        267     174
 
XhoI C'TCGA_G

Cuts at:      0     379     441 
   Size:        379      62
 

 Enzymes that do cut:

     AloI    ApaLI     ApoI     AvaI Bme1580I     BmrI     BpmI    BpuEI
    BsaXI  BsiHKAI Bsp1286I    BspHI    BspMI   BstAPI    BstYI     BtgI
    CspCI   DraIII     EaeI  Eco57MI     MscI     MslI     NspI     PfoI
     PpiI     SmlI     SspI     StyI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
     DraI     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI