(Linear) (Six-base) MAPSORT of: KIBB7437.seq  Check: 4130  from: 1  to: 714



KIBB7437.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0     113     421     714 
   Size:        113     308     293

  Fragments arranged by size: 

                 308     293     113
 
Acc65I G'GTAC_C

Cuts at:      0      20     714 
   Size:         20     694
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     714 
   Size:        641      73
 
ApaLI G'TGCA_C

Cuts at:      0      82     714 
   Size:         82     632
 
ApoI r'AATT_y

Cuts at:      0     451     714 
   Size:        451     263
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     262     295     714 
   Size:        262      33     419

  Fragments arranged by size: 

                 419     262      33
 
BanI G'GyrC_C

Cuts at:      0      20     714 
   Size:         20     694
 
BanII G_rGCy'C

Cuts at:      0     561     714 
   Size:        561     153
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     290     714 
   Size:        290     424
 
BfrBI ATG'CAT

Cuts at:      0     606     714 
   Size:        606     108
 
Bme1580I G_kGCm'C

Cuts at:      0      86     714 
   Size:         86     628
 
BsaAI yAC'GTr

Cuts at:      0     630     714 
   Size:        630      84
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      13      43     714 
   Size:         13      30     671

  Fragments arranged by size: 

                 671      30      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      21     714 
   Size:         21     693
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661     714 
   Size:        661      53
 
BsiEI CG_ry'CG

Cuts at:      0      38     714 
   Size:         38     676
 
BsiHKAI G_wGCw'C

Cuts at:      0      86     714 
   Size:         86     628
 
BsmI GAATG_Cn'

Cuts at:      0     119     714 
   Size:        119     595
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     561     714 
   Size:         86     475     153

  Fragments arranged by size: 

                 475     153      86
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     123     714 
   Size:        123     591
 
BsrGI T'GTAC_A

Cuts at:      0     195     714 
   Size:        195     519
 
BstYI r'GATC_y

Cuts at:      0     235     714 
   Size:        235     479
 
BstZ17I GTA'TAC

Cuts at:      0     422     714 
   Size:        422     292
 
DraI TTT'AAA

Cuts at:      0     266     714 
   Size:        266     448
 
EarI CTCTTCn'nnn_

Cuts at:      0     617     714 
   Size:        617      97
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      63     714 
   Size:         63     651
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     714 
   Size:        641      73
 
EcoRV GAT'ATC

Cuts at:      0     660     714 
   Size:        660      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     566     580     598     612     714 
   Size:        566      14      18      14     102

  Fragments arranged by size: 

                 566     102      18      14      14
 
HindIII A'AGCT_T

Cuts at:      0     377     714 
   Size:        377     337
 
KpnI G_GTAC'C

Cuts at:      0      24     714 
   Size:         24     690
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     714 
   Size:        299     415
 
MslI CAynn'nnrTG

Cuts at:      0      91     714 
   Size:         91     623
 
MspA1I CmG'CkG

Cuts at:      0     550     714 
   Size:        550     164
 
NsiI A_TGCA'T

Cuts at:      0     608     714 
   Size:        608     106
 
PstI C_TGCA'G

Cuts at:      0     134     555     714 
   Size:        134     421     159

  Fragments arranged by size: 

                 421     159     134
 
PvuI CG_AT'CG

Cuts at:      0      38     714 
   Size:         38     676
 
SfcI C'TryA_G

Cuts at:      0     114     130     146     395     551     714 
   Size:        114      16      16     249     156     163

  Fragments arranged by size: 

                 249     163     156     114      16      16
 
SnaBI TAC'GTA

Cuts at:      0     630     714 
   Size:        630      84
 
TatI w'GTAC_w

Cuts at:      0     195     714 
   Size:        195     519
 
XmnI GAAnn'nnTTC

Cuts at:      0     442     714 
   Size:        442     272
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    ApaLI     ApoI     BaeI     BanI    BanII
    BciVI    BfrBI Bme1580I    BsaAI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrGI    BstYI  BstZ17I     DraI
     EarI     EciI  Eco57MI    EcoRV    Hin4I  HindIII     KpnI     MmeI
     MslI   MspA1I     NsiI     PstI     PvuI     SfcI    SnaBI     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BbeI     BbsI    BbvCI     BcgI     BclI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BseYI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI