(Linear) (Six-base) MAPSORT of: KIBB7438.seq  Check: 2076  from: 1  to: 939



KIBB7438.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     170     939 
   Size:        170     769
 
AleI CACnn'nnGTG

Cuts at:      0     580     939 
   Size:        580     359
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     170     939 
   Size:        170     769
 
ApaI G_GGCC'C

Cuts at:      0     515     939 
   Size:        515     424
 
ApoI r'AATT_y

Cuts at:      0      31     939 
   Size:         31     908
 
BanII G_rGCy'C

Cuts at:      0     515     939 
   Size:        515     424
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     702     892     939 
   Size:        702     190      47

  Fragments arranged by size: 

                 702     190      47
 
BbvCI CC'TCA_GC

Cuts at:      0     904     939 
   Size:        904      35
 
BclI T'GATC_A

Cuts at:      0     668     939 
   Size:        668     271
 
BglII A'GATC_T

Cuts at:      0     302     939 
   Size:        302     637
 
Bme1580I G_kGCm'C

Cuts at:      0     515     939 
   Size:        515     424
 
Bpu10I CC'TnA_GC

Cuts at:      0     220     904     939 
   Size:        220     684      35

  Fragments arranged by size: 

                 684     220      35
 
BseYI C'CCAG_C

Cuts at:      0     416     939 
   Size:        416     523
 
BsmI GAATG_Cn'

Cuts at:      0     923     939 
   Size:        923      16
 
Bsp1286I G_dGCh'C

Cuts at:      0     515     939 
   Size:        515     424
 
BspHI T'CATG_A

Cuts at:      0     407     939 
   Size:        407     532
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     170     468     939 
   Size:        170     298     471

  Fragments arranged by size: 

                 471     298     170
 
BsrDI GCAATG_nn'

Cuts at:      0     104     928     939 
   Size:        104     824      11

  Fragments arranged by size: 

                 824     104      11
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     522     939 
   Size:        522     417
 
BstYI r'GATC_y

Cuts at:      0     302     939 
   Size:        302     637
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     939 
   Size:         85     854
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     262     363     939 
   Size:        262     101     576

  Fragments arranged by size: 

                 576     262     101
 
MslI CAynn'nnrTG

Cuts at:      0     580     939 
   Size:        580     359
 
MspA1I CmG'CkG

Cuts at:      0     498     939 
   Size:        498     441
 
PshAI GACnn'nnGTC

Cuts at:      0     739     939 
   Size:        739     200
 
PspOMI G'GGCC_C

Cuts at:      0     511     939 
   Size:        511     428
 
PstI C_TGCA'G

Cuts at:      0      71     793     939 
   Size:         71     722     146

  Fragments arranged by size: 

                 722     146      71
 
PvuII CAG'CTG

Cuts at:      0     498     939 
   Size:        498     441
 
SfcI C'TryA_G

Cuts at:      0      67     259     789     939 
   Size:         67     192     530     150

  Fragments arranged by size: 

                 530     192     150      67
 
SpeI A'CTAG_T

Cuts at:      0     486     939 
   Size:        486     453
 
StuI AGG'CCT

Cuts at:      0     783     939 
   Size:        783     156
 
XmnI GAAnn'nnTTC

Cuts at:      0      35     939 
   Size:         35     904
 

 Enzymes that do cut:

     AarI     AleI     AlfI     ApaI     ApoI    BanII     BbsI    BbvCI
     BclI    BglII Bme1580I   Bpu10I    BseYI     BsmI Bsp1286I    BspHI
    BspMI    BsrDI    BstXI    BstYI     EarI    EcoNI     MslI   MspA1I
    PshAI   PspOMI     PstI    PvuII     SfcI     SpeI     StuI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI
     SspI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI