(Linear) (Six-base) MAPSORT of: KIBB7439.seq  Check: 4692  from: 1  to: 441



KIBB7439.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      33     441 
   Size:         33     408
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     441 
   Size:        187     254
 
ApoI r'AATT_y

Cuts at:      0     100     221     441 
   Size:        100     121     220

  Fragments arranged by size: 

                 220     121     100
 
BlpI GC'TnA_GC

Cuts at:      0      64     441 
   Size:         64     377
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     321     441 
   Size:        321     120
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     441 
   Size:        173     268
 
BsaHI Gr'CG_yC

Cuts at:      0     260     441 
   Size:        260     181
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     218     441 
   Size:        218     223
 
BseYI C'CCAG_C

Cuts at:      0     242     268     441 
   Size:        242      26     173

  Fragments arranged by size: 

                 242     173      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     234     260     441 
   Size:        234      26     181

  Fragments arranged by size: 

                 234     181      26
 
BsmI GAATG_Cn'

Cuts at:      0     395     441 
   Size:        395      46
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      33     441 
   Size:         33     408
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     283     441 
   Size:        283     158
 
BstYI r'GATC_y

Cuts at:      0     170     358     378     441 
   Size:        170     188      20      63

  Fragments arranged by size: 

                 188     170      63      20
 
BtsI GCAGTG_nn'

Cuts at:      0     155     219     251     277     380     441 
   Size:        155      64      32      26     103      61

  Fragments arranged by size: 

                 155     103      64      61      32      26
 
DraIII CAC_nnn'GTG

Cuts at:      0     283     419     441 
   Size:        283     136      22

  Fragments arranged by size: 

                 283     136      22
 
EarI CTCTTCn'nnn_

Cuts at:      0     119     137     441 
   Size:        119      18     304

  Fragments arranged by size: 

                 304     119      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     321     441 
   Size:        187     134     120

  Fragments arranged by size: 

                 187     134     120
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     181     441 
   Size:        181     260
 
EcoRI G'AATT_C

Cuts at:      0     100     441 
   Size:        100     341
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      79     441 
   Size:         79     362
 
FspI TGC'GCA

Cuts at:      0     233     441 
   Size:        233     208
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     370     402     441 
   Size:        370      32      39

  Fragments arranged by size: 

                 370      39      32
 
MspA1I CmG'CkG

Cuts at:      0     229     441 
   Size:        229     212
 
PstI C_TGCA'G

Cuts at:      0     326     441 
   Size:        326     115
 
PvuII CAG'CTG

Cuts at:      0     229     441 
   Size:        229     212
 
SbfI CC_TGCA'GG

Cuts at:      0     326     441 
   Size:        326     115
 
SfcI C'TryA_G

Cuts at:      0     322     441 
   Size:        322     119
 
SmlI C'TyrA_G

Cuts at:      0     188     441 
   Size:        188     253
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     155     187     441 
   Size:        155      32     254

  Fragments arranged by size: 

                 254     155      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     100     441 
   Size:        100     341
 

 Enzymes that do cut:

     AarI     AcuI     ApoI     BlpI     BpmI    BpuEI    BsaHI    BseRI
    BseYI     BsgI     BsmI    BspMI   BstAPI    BstYI     BtsI   DraIII
     EarI  Eco57MI    EcoNI    EcoRI     FalI     FspI    Hin4I   MspA1I
     PstI    PvuII     SbfI     SfcI     SmlI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EciI  EcoICRI
 EcoO109I    EcoRV     FseI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI