Restriction map for
pF1KB7440
(Linear) (Six-base) MAPSORT of: KIBB7440.seq Check: 3795 from: 1 to: 456
KIBB7440.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 456
Size: 331 125
AflIII A'CryG_T
Cuts at: 0 368 456
Size: 368 88
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 416 456
Size: 416 40
AvaI C'yCGr_G
Cuts at: 0 146 456
Size: 146 310
BanI G'GyrC_C
Cuts at: 0 142 456
Size: 142 314
BanII G_rGCy'C
Cuts at: 0 389 456
Size: 389 67
BbeI G_GCGC'C
Cuts at: 0 146 456
Size: 146 310
Bme1580I G_kGCm'C
Cuts at: 0 441 456
Size: 441 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 456
Size: 265 191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 456
Size: 64 392
BsaAI yAC'GTr
Cuts at: 0 442 456
Size: 442 14
BsaHI Gr'CG_yC
Cuts at: 0 143 456
Size: 143 313
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 274 304 456
Size: 274 30 152
Fragments arranged by size:
274 152 30
BseYI C'CCAG_C
Cuts at: 0 151 168 456
Size: 151 17 288
Fragments arranged by size:
288 151 17
BsiHKAI G_wGCw'C
Cuts at: 0 389 456
Size: 389 67
BsiWI C'GTAC_G
Cuts at: 0 68 456
Size: 68 388
Bsp1286I G_dGCh'C
Cuts at: 0 389 441 456
Size: 389 52 15
Fragments arranged by size:
389 52 15
BsrFI r'CCGG_y
Cuts at: 0 139 456
Size: 139 317
BstXI CCAn_nnnn'nTGG
Cuts at: 0 434 456
Size: 434 22
BtgI C'CryG_G
Cuts at: 0 197 456
Size: 197 259
EaeI y'GGCC_r
Cuts at: 0 137 320 456
Size: 137 183 136
Fragments arranged by size:
183 137 136
EarI CTCTTCn'nnn_
Cuts at: 0 377 456
Size: 377 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 65 100 265 456
Size: 65 35 165 191
Fragments arranged by size:
191 165 65 35
EcoICRI GAG'CTC
Cuts at: 0 387 456
Size: 387 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 331 456
Size: 265 66 125
Fragments arranged by size:
265 125 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 78 456
Size: 78 378
EcoO109I rG'GnC_Cy
Cuts at: 0 240 456
Size: 240 216
HaeII r_GCGC'y
Cuts at: 0 146 212 456
Size: 146 66 244
Fragments arranged by size:
244 146 66
HincII GTy'rAC
Cuts at: 0 65 456
Size: 65 391
KasI G'GCGC_C
Cuts at: 0 142 456
Size: 142 314
MluI A'CGCG_T
Cuts at: 0 368 456
Size: 368 88
MslI CAynn'nnrTG
Cuts at: 0 398 456
Size: 398 58
MspA1I CmG'CkG
Cuts at: 0 26 103 185 237 342 456
Size: 26 77 82 52 105 114
Fragments arranged by size:
114 105 82 77 52 26
NaeI GCC'GGC
Cuts at: 0 141 456
Size: 141 315
NarI GG'CG_CC
Cuts at: 0 143 456
Size: 143 313
NcoI C'CATG_G
Cuts at: 0 197 456
Size: 197 259
NgoMIV G'CCGG_C
Cuts at: 0 139 456
Size: 139 317
NspI r_CATG'y
Cuts at: 0 259 456
Size: 259 197
PmlI CAC'GTG
Cuts at: 0 442 456
Size: 442 14
PpuMI rG'GwC_Cy
Cuts at: 0 240 456
Size: 240 216
PstI C_TGCA'G
Cuts at: 0 236 456
Size: 236 220
PvuII CAG'CTG
Cuts at: 0 185 456
Size: 185 271
SacI G_AGCT'C
Cuts at: 0 389 456
Size: 389 67
SapI GCTCTTCn'nnn_
Cuts at: 0 377 456
Size: 377 79
ScaI AGT'ACT
Cuts at: 0 57 456
Size: 57 399
SfcI C'TryA_G
Cuts at: 0 232 294 456
Size: 232 62 162
Fragments arranged by size:
232 162 62
SfoI GGC'GCC
Cuts at: 0 144 456
Size: 144 312
SmlI C'TyrA_G
Cuts at: 0 43 456
Size: 43 413
StyI C'CwwG_G
Cuts at: 0 197 456
Size: 197 259
TatI w'GTAC_w
Cuts at: 0 55 456
Size: 55 401
Enzymes that do cut:
AcuI AflIII AlfI AvaI BanI BanII BbeI Bme1580I
BpmI BpuEI BsaAI BsaHI BsaXI BseYI BsiHKAI BsiWI
Bsp1286I BsrFI BstXI BtgI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII HincII KasI MluI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PmlI PpuMI
PstI PvuII SacI SapI ScaI SfcI SfoI SmlI
StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BsaI BsaBI BsaWI BseRI BsgI
BsiEI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI KpnI MfeI MmeI MscI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI