Restriction map for
pF1KB7441
(Linear) (Six-base) MAPSORT of: KIBB7441.seq Check: 6201 from: 1 to: 645
KIBB7441.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AatII G_ACGT'C
Cuts at: 0 599 645
Size: 599 46
AccI GT'mk_AC
Cuts at: 0 173 645
Size: 173 472
AflIII A'CryG_T
Cuts at: 0 54 645
Size: 54 591
AleI CACnn'nnGTG
Cuts at: 0 55 645
Size: 55 590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 47 645
Size: 47 598
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 364 396 645
Size: 364 32 249
Fragments arranged by size:
364 249 32
ApaLI G'TGCA_C
Cuts at: 0 3 58 645
Size: 3 55 587
Fragments arranged by size:
587 55 3
AseI AT'TA_AT
Cuts at: 0 614 645
Size: 614 31
BanII G_rGCy'C
Cuts at: 0 100 593 645
Size: 100 493 52
Fragments arranged by size:
493 100 52
BclI T'GATC_A
Cuts at: 0 546 645
Size: 546 99
BglII A'GATC_T
Cuts at: 0 28 645
Size: 28 617
Bme1580I G_kGCm'C
Cuts at: 0 7 62 645
Size: 7 55 583
Fragments arranged by size:
583 55 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 645
Size: 574 71
Bpu10I CC'TnA_GC
Cuts at: 0 263 645
Size: 263 382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 645
Size: 435 210
BsaI GGTCTCn'nnnn_
Cuts at: 0 190 645
Size: 190 455
BsaAI yAC'GTr
Cuts at: 0 369 645
Size: 369 276
BsaHI Gr'CG_yC
Cuts at: 0 596 645
Size: 596 49
BsiHKAI G_wGCw'C
Cuts at: 0 7 62 169 593 645
Size: 7 55 107 424 52
Fragments arranged by size:
424 107 55 52 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 62 100 169 593 645
Size: 7 55 38 69 424 52
Fragments arranged by size:
424 69 55 52 38 7
BspHI T'CATG_A
Cuts at: 0 624 645
Size: 624 21
BsrDI GCAATG_nn'
Cuts at: 0 431 478 645
Size: 431 47 167
Fragments arranged by size:
431 167 47
BstXI CCAn_nnnn'nTGG
Cuts at: 0 92 645
Size: 92 553
BstYI r'GATC_y
Cuts at: 0 28 645
Size: 28 617
BtgI C'CryG_G
Cuts at: 0 106 180 645
Size: 106 74 465
Fragments arranged by size:
465 106 74
BtsI GCAGTG_nn'
Cuts at: 0 495 496 645
Size: 495 1 149
Fragments arranged by size:
495 149 1
EarI CTCTTCn'nnn_
Cuts at: 0 221 645
Size: 221 424
EcoICRI GAG'CTC
Cuts at: 0 591 645
Size: 591 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 645
Size: 574 71
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 288 645
Size: 288 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 477 509 645
Size: 477 32 136
Fragments arranged by size:
477 136 32
MslI CAynn'nnrTG
Cuts at: 0 55 71 77 211 645
Size: 55 16 6 134 434
Fragments arranged by size:
434 134 55 16 6
MspA1I CmG'CkG
Cuts at: 0 552 645
Size: 552 93
NspI r_CATG'y
Cuts at: 0 58 216 456 645
Size: 58 158 240 189
Fragments arranged by size:
240 189 158 58
PasI CC'CwG_GG
Cuts at: 0 582 645
Size: 582 63
PciI A'CATG_T
Cuts at: 0 54 645
Size: 54 591
PstI C_TGCA'G
Cuts at: 0 314 502 645
Size: 314 188 143
Fragments arranged by size:
314 188 143
PvuII CAG'CTG
Cuts at: 0 552 645
Size: 552 93
SacI G_AGCT'C
Cuts at: 0 593 645
Size: 593 52
SfcI C'TryA_G
Cuts at: 0 310 498 645
Size: 310 188 147
Fragments arranged by size:
310 188 147
SmlI C'TyrA_G
Cuts at: 0 414 645
Size: 414 231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 579 645
Size: 354 225 66
Fragments arranged by size:
354 225 66
ZraI GAC'GTC
Cuts at: 0 597 645
Size: 597 48
Enzymes that do cut:
AatII AccI AflIII AleI AlfI AloI ApaLI AseI
BanII BclI BglII Bme1580I BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsiHKAI Bsp1286I BspHI BsrDI BstXI BstYI
BtgI BtsI EarI EcoICRI Eco57MI FalI Hin4I MslI
MspA1I NspI PasI PciI PstI PvuII SacI SfcI
SmlI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AlwNI ApaI ApoI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI