Restriction map for
pF1KB7442
(Linear) (Six-base) MAPSORT of: KIBB7442.seq Check: 9743 from: 1 to: 1035
KIBB7442.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AatII G_ACGT'C
Cuts at: 0 531 1035
Size: 531 504
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 1035
Size: 785 250
AflII C'TTAA_G
Cuts at: 0 717 1035
Size: 717 318
AhdI GACnn_n'nnGTC
Cuts at: 0 527 1035
Size: 527 508
AlwNI CAG_nnn'CTG
Cuts at: 0 808 1035
Size: 808 227
ApaLI G'TGCA_C
Cuts at: 0 259 1035
Size: 259 776
ApoI r'AATT_y
Cuts at: 0 1021 1035
Size: 1021 14
BanII G_rGCy'C
Cuts at: 0 439 898 1035
Size: 439 459 137
Fragments arranged by size:
459 439 137
BbsI GAAGACnn'nnnn_
Cuts at: 0 934 1035
Size: 934 101
BfrBI ATG'CAT
Cuts at: 0 3 1035
Size: 3 1032
Bme1580I G_kGCm'C
Cuts at: 0 263 1035
Size: 263 772
BmrI ACTGGGnnnn_n'
Cuts at: 0 273 1035
Size: 273 762
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1028 1035
Size: 1028 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 394 1035
Size: 114 280 641
Fragments arranged by size:
641 280 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 845 1035
Size: 845 190
BsaBI GATnn'nnATC
Cuts at: 0 589 1035
Size: 589 446
BsaHI Gr'CG_yC
Cuts at: 0 528 1035
Size: 528 507
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 650 1035
Size: 181 469 385
Fragments arranged by size:
469 385 181
BseYI C'CCAG_C
Cuts at: 0 121 349 557 654 808 1035
Size: 121 228 208 97 154 227
Fragments arranged by size:
228 227 208 154 121 97
BsiHKAI G_wGCw'C
Cuts at: 0 203 263 1035
Size: 203 60 772
Fragments arranged by size:
772 203 60
BsmI GAATG_Cn'
Cuts at: 0 451 1035
Size: 451 584
BsmBI CGTCTCn'nnnn_
Cuts at: 0 535 1035
Size: 535 500
Bsp1286I G_dGCh'C
Cuts at: 0 203 263 439 898 1035
Size: 203 60 176 459 137
Fragments arranged by size:
459 203 176 137 60
BsrDI GCAATG_nn'
Cuts at: 0 243 1035
Size: 243 792
BsrGI T'GTAC_A
Cuts at: 0 290 1035
Size: 290 745
BstXI CCAn_nnnn'nTGG
Cuts at: 0 357 546 1035
Size: 357 189 489
Fragments arranged by size:
489 357 189
BstYI r'GATC_y
Cuts at: 0 185 622 1035
Size: 185 437 413
Fragments arranged by size:
437 413 185
BtsI GCAGTG_nn'
Cuts at: 0 961 1035
Size: 961 74
ClaI AT'CG_AT
Cuts at: 0 477 1035
Size: 477 558
DraI TTT'AAA
Cuts at: 0 26 1035
Size: 26 1009
EaeI y'GGCC_r
Cuts at: 0 359 1035
Size: 359 676
EarI CTCTTCn'nnn_
Cuts at: 0 208 668 754 1035
Size: 208 460 86 281
Fragments arranged by size:
460 281 208 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 394 785 1035
Size: 114 280 391 250
Fragments arranged by size:
391 280 250 114
EcoO109I rG'GnC_Cy
Cuts at: 0 688 761 795 1035
Size: 688 73 34 240
Fragments arranged by size:
688 240 73 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 839 1035
Size: 839 196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 62 513 545 921 953 969 1001
Size: 30 32 451 32 376 32 16 32
Cuts at: 1001 1035
Size: 34
Fragments arranged by size:
451 376 34 32 32 32 32 30
16
HincII GTy'rAC
Cuts at: 0 597 1035
Size: 597 438
MfeI C'AATT_G
Cuts at: 0 552 1035
Size: 552 483
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 1035
Size: 535 500
MscI TGG'CCA
Cuts at: 0 361 1035
Size: 361 674
MspA1I CmG'CkG
Cuts at: 0 808 1035
Size: 808 227
NsiI A_TGCA'T
Cuts at: 0 5 1035
Size: 5 1030
NspI r_CATG'y
Cuts at: 0 357 1035
Size: 357 678
PasI CC'CwG_GG
Cuts at: 0 173 1035
Size: 173 862
PpuMI rG'GwC_Cy
Cuts at: 0 761 795 1035
Size: 761 34 240
Fragments arranged by size:
761 240 34
PvuII CAG'CTG
Cuts at: 0 808 1035
Size: 808 227
SanDI GG'GwC_CC
Cuts at: 0 795 1035
Size: 795 240
SapI GCTCTTCn'nnn_
Cuts at: 0 208 1035
Size: 208 827
ScaI AGT'ACT
Cuts at: 0 878 1035
Size: 878 157
SfcI C'TryA_G
Cuts at: 0 440 1035
Size: 440 595
SmlI C'TyrA_G
Cuts at: 0 717 1035
Size: 717 318
SphI G_CATG'C
Cuts at: 0 357 1035
Size: 357 678
StyI C'CwwG_G
Cuts at: 0 431 1035
Size: 431 604
SwaI ATTT'AAAT
Cuts at: 0 26 1035
Size: 26 1009
TatI w'GTAC_w
Cuts at: 0 290 876 1035
Size: 290 586 159
Fragments arranged by size:
586 290 159
XmnI GAAnn'nnTTC
Cuts at: 0 109 1035
Size: 109 926
ZraI GAC'GTC
Cuts at: 0 529 1035
Size: 529 506
Enzymes that do cut:
AatII AcuI AflII AhdI AlwNI ApaLI ApoI BanII
BbsI BfrBI Bme1580I BmrI BplI BpmI BsaI BsaBI
BsaHI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BsrDI
BsrGI BstXI BstYI BtsI ClaI DraI EaeI EarI
Eco57MI EcoO109I FalI Hin4I HincII MfeI MmeI MscI
MspA1I NsiI NspI PasI PpuMI PvuII SanDI SapI
ScaI SfcI SmlI SphI StyI SwaI TatI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflIII AgeI AleI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaAI
BsaWI BsaXI BsgI BsiEI BsiWI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MluI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI