Restriction map for
pF1KB7443
(Linear) (Six-base) MAPSORT of: KIBB7443.seq Check: 7793 from: 1 to: 876
KIBB7443.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 269 377 850 876
Size: 269 108 473 26
Fragments arranged by size:
473 269 108 26
AatII G_ACGT'C
Cuts at: 0 662 876
Size: 662 214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 876
Size: 337 539
AflII C'TTAA_G
Cuts at: 0 30 876
Size: 30 846
AleI CACnn'nnGTG
Cuts at: 0 148 676 876
Size: 148 528 200
Fragments arranged by size:
528 200 148
AlwNI CAG_nnn'CTG
Cuts at: 0 633 876
Size: 633 243
AvaI C'yCGr_G
Cuts at: 0 622 828 876
Size: 622 206 48
Fragments arranged by size:
622 206 48
BanI G'GyrC_C
Cuts at: 0 365 391 876
Size: 365 26 485
Fragments arranged by size:
485 365 26
BanII G_rGCy'C
Cuts at: 0 18 218 876
Size: 18 200 658
Fragments arranged by size:
658 200 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 364 398 876
Size: 364 34 478
Fragments arranged by size:
478 364 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 713 876
Size: 713 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 876
Size: 155 721
BsaHI Gr'CG_yC
Cuts at: 0 659 876
Size: 659 217
BseYI C'CCAG_C
Cuts at: 0 298 876
Size: 298 578
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 802 876
Size: 562 240 74
Fragments arranged by size:
562 240 74
BsiEI CG_ry'CG
Cuts at: 0 537 876
Size: 537 339
BsiWI C'GTAC_G
Cuts at: 0 154 876
Size: 154 722
BsmBI CGTCTCn'nnnn_
Cuts at: 0 351 876
Size: 351 525
Bsp1286I G_dGCh'C
Cuts at: 0 18 218 876
Size: 18 200 658
Fragments arranged by size:
658 200 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 269 377 850 876
Size: 269 108 473 26
Fragments arranged by size:
473 269 108 26
BsrBI CCG'CTC
Cuts at: 0 239 609 876
Size: 239 370 267
Fragments arranged by size:
370 267 239
BsrFI r'CCGG_y
Cuts at: 0 114 455 876
Size: 114 341 421
Fragments arranged by size:
421 341 114
BtgI C'CryG_G
Cuts at: 0 181 387 566 876
Size: 181 206 179 310
Fragments arranged by size:
310 206 181 179
ClaI AT'CG_AT
Cuts at: 0 89 876
Size: 89 787
DraIII CAC_nnn'GTG
Cuts at: 0 64 876
Size: 64 812
EaeI y'GGCC_r
Cuts at: 0 158 876
Size: 158 718
EarI CTCTTCn'nnn_
Cuts at: 0 313 876
Size: 313 563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 337 876
Size: 155 182 539
Fragments arranged by size:
539 182 155
EcoO109I rG'GnC_Cy
Cuts at: 0 486 504 588 876
Size: 486 18 84 288
Fragments arranged by size:
486 288 84 18
HaeII r_GCGC'y
Cuts at: 0 576 876
Size: 576 300
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 696 728 876
Size: 62 32 602 32 148
Fragments arranged by size:
602 148 62 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 876
Size: 572 304
MslI CAynn'nnrTG
Cuts at: 0 148 415 676 719 876
Size: 148 267 261 43 157
Fragments arranged by size:
267 261 157 148 43
MspA1I CmG'CkG
Cuts at: 0 389 558 594 816 876
Size: 389 169 36 222 60
Fragments arranged by size:
389 222 169 60 36
NaeI GCC'GGC
Cuts at: 0 457 876
Size: 457 419
NgoMIV G'CCGG_C
Cuts at: 0 455 876
Size: 455 421
NspI r_CATG'y
Cuts at: 0 528 876
Size: 528 348
PasI CC'CwG_GG
Cuts at: 0 747 876
Size: 747 129
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 876
Size: 252 624
PpuMI rG'GwC_Cy
Cuts at: 0 486 588 876
Size: 486 102 288
Fragments arranged by size:
486 288 102
PstI C_TGCA'G
Cuts at: 0 285 876
Size: 285 591
PvuII CAG'CTG
Cuts at: 0 558 816 876
Size: 558 258 60
Fragments arranged by size:
558 258 60
SacII CC_GC'GG
Cuts at: 0 390 876
Size: 390 486
SfcI C'TryA_G
Cuts at: 0 281 876
Size: 281 595
SmaI CCC'GGG
Cuts at: 0 830 876
Size: 830 46
SmlI C'TyrA_G
Cuts at: 0 30 876
Size: 30 846
SphI G_CATG'C
Cuts at: 0 528 876
Size: 528 348
StyI C'CwwG_G
Cuts at: 0 439 876
Size: 439 437
Tth111I GACn'n_nGTC
Cuts at: 0 484 876
Size: 484 392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 678 876
Size: 678 198
XmaI C'CCGG_G
Cuts at: 0 828 876
Size: 828 48
ZraI GAC'GTC
Cuts at: 0 660 876
Size: 660 216
Enzymes that do cut:
AarI AatII AcuI AflII AleI AlwNI AvaI BanI
BanII BcgI BmrI BpmI BsaHI BseYI BsgI BsiEI
BsiWI BsmBI Bsp1286I BspMI BsrBI BsrFI BtgI ClaI
DraIII EaeI EarI Eco57MI EcoO109I HaeII Hin4I MmeI
MslI MspA1I NaeI NgoMIV NspI PasI PflMI PpuMI
PstI PvuII SacII SfcI SmaI SmlI SphI StyI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiHKAI
BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI TaqII TatI TstI XbaI XhoI XmnI