(Linear) (Six-base) MAPSORT of: KIBB7445.seq  Check: 9916  from: 1  to: 1494



KIBB7445.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0     518    1494 
   Size:        518     976
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     736     821    1494 
   Size:         42     694      85     673

  Fragments arranged by size: 

                 694     673      85      42
 
ApaI G_GGCC'C

Cuts at:      0     268    1494 
   Size:        268    1226
 
ApoI r'AATT_y

Cuts at:      0     643    1494 
   Size:        643     851
 
AseI AT'TA_AT

Cuts at:      0     535    1494 
   Size:        535     959
 
BanII G_rGCy'C

Cuts at:      0     268    1494 
   Size:        268    1226
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     340     736    1494 
   Size:        340     396     758

  Fragments arranged by size: 

                 758     396     340
 
BbvCI CC'TCA_GC

Cuts at:      0       8    1494 
   Size:          8    1486
 
BglI GCCn_nnn'nGGC

Cuts at:      0     942    1494 
   Size:        942     552
 
BglII A'GATC_T

Cuts at:      0     192    1494 
   Size:        192    1302
 
Bme1580I G_kGCm'C

Cuts at:      0     268    1494 
   Size:        268    1226
 
BmgBI CAC'GTC

Cuts at:      0     180    1494 
   Size:        180    1314
 
BmtI G_CTAG'C

Cuts at:      0    1370    1494 
   Size:       1370     124
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     262     320     419    1494 
   Size:        262      58      99    1075

  Fragments arranged by size: 

                1075     262      99      58
 
Bpu10I CC'TnA_GC

Cuts at:      0       8     259    1494 
   Size:          8     251    1235

  Fragments arranged by size: 

                1235     251       8
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     761     968    1494 
   Size:        761     207     526

  Fragments arranged by size: 

                 761     526     207
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     134    1494 
   Size:        134    1360
 
BsaBI GATnn'nnATC

Cuts at:      0     473    1494 
   Size:        473    1021
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     157     187    1494 
   Size:        157      30    1307

  Fragments arranged by size: 

                1307     157      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     268    1494 
   Size:        268    1226
 
BsrBI CCG'CTC

Cuts at:      0     241    1494 
   Size:        241    1253
 
BsrDI GCAATG_nn'

Cuts at:      0    1104    1324    1494 
   Size:       1104     220     170

  Fragments arranged by size: 

                1104     220     170
 
BsrGI T'GTAC_A

Cuts at:      0     615    1494 
   Size:        615     879
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      80    1494 
   Size:         80    1414
 
BstYI r'GATC_y

Cuts at:      0     192     243    1494 
   Size:        192      51    1251

  Fragments arranged by size: 

                1251     192      51
 
BtsI GCAGTG_nn'

Cuts at:      0     547    1494 
   Size:        547     947
 
DraI TTT'AAA

Cuts at:      0     671    1494 
   Size:        671     823
 
EaeI y'GGCC_r

Cuts at:      0     539    1264    1494 
   Size:        539     725     230

  Fragments arranged by size: 

                 725     539     230
 
EarI CTCTTCn'nnn_

Cuts at:      0     289     888     926    1169    1494 
   Size:        289     599      38     243     325

  Fragments arranged by size: 

                 599     325     289     243      38
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     736     821    1494 
   Size:         42     694      85     673

  Fragments arranged by size: 

                 694     673      85      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     102    1494 
   Size:        102    1392
 
EcoO109I rG'GnC_Cy

Cuts at:      0     264     265    1494 
   Size:        264       1    1229

  Fragments arranged by size: 

                1229     264       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      66    1494 
   Size:         66    1428
 
HaeII r_GCGC'y

Cuts at:      0     158    1494 
   Size:        158    1336
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     559     591    1494 
   Size:        559      32     903

  Fragments arranged by size: 

                 903     559      32
 
HincII GTy'rAC

Cuts at:      0     370    1494 
   Size:        370    1124
 
MscI TGG'CCA

Cuts at:      0     541    1266    1494 
   Size:        541     725     228

  Fragments arranged by size: 

                 725     541     228
 
MslI CAynn'nnrTG

Cuts at:      0     253    1494 
   Size:        253    1241
 
NheI G'CTAG_C

Cuts at:      0    1366    1494 
   Size:       1366     128
 
PfoI T'CCnGG_A

Cuts at:      0    1382    1494 
   Size:       1382     112
 
PspOMI G'GGCC_C

Cuts at:      0     264    1494 
   Size:        264    1230
 
PstI C_TGCA'G

Cuts at:      0     106    1494 
   Size:        106    1388
 
SapI GCTCTTCn'nnn_

Cuts at:      0     926    1494 
   Size:        926     568
 
SfcI C'TryA_G

Cuts at:      0     102    1494 
   Size:        102    1392
 
SmlI C'TyrA_G

Cuts at:      0     776     983    1494 
   Size:        776     207     511

  Fragments arranged by size: 

                 776     511     207
 
SspI AAT'ATT

Cuts at:      0     762    1494 
   Size:        762     732
 
StuI AGG'CCT

Cuts at:      0    1188    1494 
   Size:       1188     306
 
StyI C'CwwG_G

Cuts at:      0     169    1494 
   Size:        169    1325
 
SwaI ATTT'AAAT

Cuts at:      0     671    1494 
   Size:        671     823
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     118    1494 
   Size:        118    1376
 
TatI w'GTAC_w

Cuts at:      0     615    1482    1494 
   Size:        615     867      12

  Fragments arranged by size: 

                 867     615      12
 
Tth111I GACn'n_nGTC

Cuts at:      0     365    1494 
   Size:        365    1129
 

 Enzymes that do cut:

     AccI     AcuI     ApaI     ApoI     AseI    BanII     BbsI    BbvCI
     BglI    BglII Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaXI Bsp1286I    BsrBI    BsrDI    BsrGI   BstAPI
    BstYI     BtsI     DraI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I   HincII     MscI     MslI     NheI     PfoI
   PspOMI     PstI     SapI     SfcI     SmlI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
     BclI    BfrBI     BlpI     BmrI     BpmI    BsaAI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrFI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI