(Linear) (Six-base) MAPSORT of: KIBB7446.seq  Check: 3614  from: 1  to: 933



KIBB7446.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     933 
   Size:         43     890
 
AflIII A'CryG_T

Cuts at:      0     644     933 
   Size:        644     289
 
ApoI r'AATT_y

Cuts at:      0     322     933 
   Size:        322     611
 
BclI T'GATC_A

Cuts at:      0     513     933 
   Size:        513     420
 
BfrBI ATG'CAT

Cuts at:      0     221     933 
   Size:        221     712
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     933 
   Size:        466     467
 
BsaAI yAC'GTr

Cuts at:      0      92     933 
   Size:         92     841
 
BsaWI w'CCGG_w

Cuts at:      0     179     933 
   Size:        179     754
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     469     933 
   Size:        469     464
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     396     933 
   Size:        302      94     537

  Fragments arranged by size: 

                 537     302      94
 
BspEI T'CCGG_A

Cuts at:      0     179     933 
   Size:        179     754
 
BspHI T'CATG_A

Cuts at:      0     395     933 
   Size:        395     538
 
BsrGI T'GTAC_A

Cuts at:      0     647     933 
   Size:        647     286
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     234     933 
   Size:        234     699
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     466     933 
   Size:         43     423     467

  Fragments arranged by size: 

                 467     423      43
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     933 
   Size:        527     406
 
MslI CAynn'nnrTG

Cuts at:      0     313     933 
   Size:        313     620
 
MspA1I CmG'CkG

Cuts at:      0     486     933 
   Size:        486     447
 
NsiI A_TGCA'T

Cuts at:      0     223     933 
   Size:        223     710
 
NspI r_CATG'y

Cuts at:      0     527     648     933 
   Size:        527     121     285

  Fragments arranged by size: 

                 527     285     121
 
PciI A'CATG_T

Cuts at:      0     644     933 
   Size:        644     289
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      12     933 
   Size:         12     921
 
PstI C_TGCA'G

Cuts at:      0     158     933 
   Size:        158     775
 
PvuII CAG'CTG

Cuts at:      0     486     933 
   Size:        486     447
 
SfcI C'TryA_G

Cuts at:      0     154     933 
   Size:        154     779
 
TatI w'GTAC_w

Cuts at:      0     647     933 
   Size:        647     286
 
XmnI GAAnn'nnTTC

Cuts at:      0     850     933 
   Size:        850      83
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     BclI    BfrBI     BpmI    BsaAI    BsaWI
    BseRI     BsgI    BspEI    BspHI    BsrGI    BstXI  Eco57MI     MmeI
     MslI   MspA1I     NsiI     NspI     PciI    PflMI     PstI    PvuII
     SfcI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI