Restriction map for
pF1KB7447
(Linear) (Six-base) MAPSORT of: KIBB7447.seq Check: 7692 from: 1 to: 816
KIBB7447.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 816
Size: 655 161
AleI CACnn'nnGTG
Cuts at: 0 451 816
Size: 451 365
AlwNI CAG_nnn'CTG
Cuts at: 0 194 679 816
Size: 194 485 137
Fragments arranged by size:
485 194 137
ApoI r'AATT_y
Cuts at: 0 768 816
Size: 768 48
BanII G_rGCy'C
Cuts at: 0 223 301 323 816
Size: 223 78 22 493
Fragments arranged by size:
493 223 78 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 579 816
Size: 579 237
BmrI ACTGGGnnnn_n'
Cuts at: 0 257 816
Size: 257 559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 712 816
Size: 91 621 104
Fragments arranged by size:
621 104 91
BsaI GGTCTCn'nnnn_
Cuts at: 0 340 816
Size: 340 476
BsaAI yAC'GTr
Cuts at: 0 435 597 816
Size: 435 162 219
Fragments arranged by size:
435 219 162
BsaWI w'CCGG_w
Cuts at: 0 365 482 816
Size: 365 117 334
Fragments arranged by size:
365 334 117
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 569 599 816
Size: 569 30 217
Fragments arranged by size:
569 217 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 816
Size: 228 588
BsiHKAI G_wGCw'C
Cuts at: 0 301 323 816
Size: 301 22 493
Fragments arranged by size:
493 301 22
Bsp1286I G_dGCh'C
Cuts at: 0 223 301 323 816
Size: 223 78 22 493
Fragments arranged by size:
493 223 78 22
BspEI T'CCGG_A
Cuts at: 0 365 816
Size: 365 451
BsrDI GCAATG_nn'
Cuts at: 0 516 816
Size: 516 300
BsrGI T'GTAC_A
Cuts at: 0 660 816
Size: 660 156
BstXI CCAn_nnnn'nTGG
Cuts at: 0 690 816
Size: 690 126
BtsI GCAGTG_nn'
Cuts at: 0 198 253 677 772 816
Size: 198 55 424 95 44
Fragments arranged by size:
424 198 95 55 44
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1 36 816
Size: 1 35 780
Fragments arranged by size:
780 35 1
DraI TTT'AAA
Cuts at: 0 32 816
Size: 32 784
DrdI GACnn_nn'nnGTC
Cuts at: 0 520 816
Size: 520 296
EaeI y'GGCC_r
Cuts at: 0 532 816
Size: 532 284
EarI CTCTTCn'nnn_
Cuts at: 0 209 816
Size: 209 607
EcoICRI GAG'CTC
Cuts at: 0 299 321 816
Size: 299 22 495
Fragments arranged by size:
495 299 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 655 712 816
Size: 91 564 57 104
Fragments arranged by size:
564 104 91 57
EcoRV GAT'ATC
Cuts at: 0 56 816
Size: 56 760
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 351 816
Size: 351 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 569 601 816
Size: 569 32 215
Fragments arranged by size:
569 215 32
MscI TGG'CCA
Cuts at: 0 534 816
Size: 534 282
MslI CAynn'nnrTG
Cuts at: 0 451 816
Size: 451 365
MspA1I CmG'CkG
Cuts at: 0 490 816
Size: 490 326
NruI TCG'CGA
Cuts at: 0 442 816
Size: 442 374
PstI C_TGCA'G
Cuts at: 0 190 786 816
Size: 190 596 30
Fragments arranged by size:
596 190 30
SacI G_AGCT'C
Cuts at: 0 301 323 816
Size: 301 22 493
Fragments arranged by size:
493 301 22
SfcI C'TryA_G
Cuts at: 0 186 382 782 816
Size: 186 196 400 34
Fragments arranged by size:
400 196 186 34
SnaBI TAC'GTA
Cuts at: 0 597 816
Size: 597 219
SspI AAT'ATT
Cuts at: 0 413 816
Size: 413 403
StuI AGG'CCT
Cuts at: 0 640 816
Size: 640 176
TatI w'GTAC_w
Cuts at: 0 660 816
Size: 660 156
XmnI GAAnn'nnTTC
Cuts at: 0 412 700 816
Size: 412 288 116
Fragments arranged by size:
412 288 116
Enzymes that do cut:
AcuI AleI AlwNI ApoI BanII BbsI BmrI BpmI
BsaI BsaAI BsaWI BsaXI BsgI BsiHKAI Bsp1286I BspEI
BsrDI BsrGI BstXI BtsI CspCI DraI DrdI EaeI
EarI EcoICRI Eco57MI EcoRV FalI Hin4I MscI MslI
MspA1I NruI PstI SacI SfcI SnaBI SspI StuI
TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI Bpu10I BpuEI BsaBI BsaHI BseRI
BseYI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI DraIII EagI EciI EcoNI
EcoO109I EcoRI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI ZraI