Restriction map for
pF1KB7448
(Linear) (Six-base) MAPSORT of: KIBB7448.seq Check: 7864 from: 1 to: 972
KIBB7448.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
Acc65I G'GTAC_C
Cuts at: 0 243 972
Size: 243 729
AflIII A'CryG_T
Cuts at: 0 730 972
Size: 730 242
AleI CACnn'nnGTG
Cuts at: 0 337 832 972
Size: 337 495 140
Fragments arranged by size:
495 337 140
ApoI r'AATT_y
Cuts at: 0 413 972
Size: 413 559
AvaI C'yCGr_G
Cuts at: 0 270 972
Size: 270 702
BanI G'GyrC_C
Cuts at: 0 243 972
Size: 243 729
BanII G_rGCy'C
Cuts at: 0 185 637 972
Size: 185 452 335
Fragments arranged by size:
452 335 185
BciVI GTATCCnnnnn_n'
Cuts at: 0 70 972
Size: 70 902
BclI T'GATC_A
Cuts at: 0 857 972
Size: 857 115
Bme1580I G_kGCm'C
Cuts at: 0 593 821 972
Size: 593 228 151
Fragments arranged by size:
593 228 151
BmtI G_CTAG'C
Cuts at: 0 844 972
Size: 844 128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 972
Size: 934 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 972
Size: 410 562
BsaAI yAC'GTr
Cuts at: 0 855 972
Size: 855 117
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 225 687 972
Size: 225 462 285
Fragments arranged by size:
462 285 225
BseYI C'CCAG_C
Cuts at: 0 3 435 592 864 972
Size: 3 432 157 272 108
Fragments arranged by size:
432 272 157 108 3
BsmBI CGTCTCn'nnnn_
Cuts at: 0 337 972
Size: 337 635
Bsp1286I G_dGCh'C
Cuts at: 0 185 593 637 821 972
Size: 185 408 44 184 151
Fragments arranged by size:
408 185 184 151 44
BspHI T'CATG_A
Cuts at: 0 476 972
Size: 476 496
BsrDI GCAATG_nn'
Cuts at: 0 650 972
Size: 650 322
BsrGI T'GTAC_A
Cuts at: 0 113 972
Size: 113 859
BstAPI GCAn_nnn'nTGC
Cuts at: 0 266 972
Size: 266 706
BstXI CCAn_nnnn'nTGG
Cuts at: 0 339 491 972
Size: 339 152 481
Fragments arranged by size:
481 339 152
BtgI C'CryG_G
Cuts at: 0 239 362 972
Size: 239 123 610
Fragments arranged by size:
610 239 123
DraI TTT'AAA
Cuts at: 0 875 972
Size: 875 97
DraIII CAC_nnn'GTG
Cuts at: 0 106 972
Size: 106 866
EaeI y'GGCC_r
Cuts at: 0 449 563 972
Size: 449 114 409
Fragments arranged by size:
449 409 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 972
Size: 934 38
EcoRI G'AATT_C
Cuts at: 0 413 972
Size: 413 559
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 486 489 518 521 682 714 972
Size: 486 3 29 3 161 32 258
Fragments arranged by size:
486 258 161 32 29 3 3
KpnI G_GTAC'C
Cuts at: 0 247 972
Size: 247 725
MfeI C'AATT_G
Cuts at: 0 907 972
Size: 907 65
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 972
Size: 472 500
MscI TGG'CCA
Cuts at: 0 565 972
Size: 565 407
MslI CAynn'nnrTG
Cuts at: 0 226 310 337 475 832 972
Size: 226 84 27 138 357 140
Fragments arranged by size:
357 226 140 138 84 27
MspA1I CmG'CkG
Cuts at: 0 337 972
Size: 337 635
NcoI C'CATG_G
Cuts at: 0 362 972
Size: 362 610
NheI G'CTAG_C
Cuts at: 0 840 972
Size: 840 132
NspI r_CATG'y
Cuts at: 0 578 734 972
Size: 578 156 238
Fragments arranged by size:
578 238 156
PciI A'CATG_T
Cuts at: 0 730 972
Size: 730 242
PflMI CCAn_nnn'nTGG
Cuts at: 0 27 972
Size: 27 945
PvuII CAG'CTG
Cuts at: 0 337 972
Size: 337 635
SfcI C'TryA_G
Cuts at: 0 603 972
Size: 603 369
SmlI C'TyrA_G
Cuts at: 0 425 972
Size: 425 547
StyI C'CwwG_G
Cuts at: 0 362 972
Size: 362 610
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 844 972
Size: 844 128
TatI w'GTAC_w
Cuts at: 0 113 761 972
Size: 113 648 211
Fragments arranged by size:
648 211 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 861 893 972
Size: 861 32 79
Fragments arranged by size:
861 79 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 909 972
Size: 909 63
Enzymes that do cut:
Acc65I AflIII AleI ApoI AvaI BanI BanII BciVI
BclI Bme1580I BmtI BpmI BpuEI BsaAI BseRI BseYI
BsmBI Bsp1286I BspHI BsrDI BsrGI BstAPI BstXI BtgI
DraI DraIII EaeI Eco57MI EcoRI Hin4I KpnI MfeI
MmeI MscI MslI MspA1I NcoI NheI NspI PciI
PflMI PvuII SfcI SmlI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BfrBI BglI BglII BlpI BmgBI BmrI BplI Bpu10I
BsaI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmI BspEI BspMI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MluI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI