(Linear) (Six-base) MAPSORT of: KIBB7449.seq  Check: 2860  from: 1  to: 564



KIBB7449.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

ApoI r'AATT_y

Cuts at:      0     121     564 
   Size:        121     443
 
AvaI C'yCGr_G

Cuts at:      0     337     564 
   Size:        337     227
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       1      35      54      64      88      98     315     349 
   Size:          1      34      19      10      24      10     217      34

Cuts at:    349     357     391     564 
   Size:          8      34     173

  Fragments arranged by size: 

                 217     173      34      34      34      24      19      10
                  10       8       1
 
BglII A'GATC_T

Cuts at:      0     460     564 
   Size:        460     104
 
Bme1580I G_kGCm'C

Cuts at:      0     421     564 
   Size:        421     143
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     564 
   Size:        295     269
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      21     564 
   Size:         21     543
 
BsaHI Gr'CG_yC

Cuts at:      0      14     564 
   Size:         14     550
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       2     564 
   Size:          2     562
 
Bsp1286I G_dGCh'C

Cuts at:      0     421     564 
   Size:        421     143
 
BssSI C'ACGA_G

Cuts at:      0     454     564 
   Size:        454     110
 
BstBI TT'CG_AA

Cuts at:      0     367     564 
   Size:        367     197
 
BstYI r'GATC_y

Cuts at:      0     460     564 
   Size:        460     104
 
Bsu36I CC'TnA_GG

Cuts at:      0     548     564 
   Size:        548      16
 
BtgI C'CryG_G

Cuts at:      0     267     564 
   Size:        267     297
 
EarI CTCTTCn'nnn_

Cuts at:      0      52      98     148     527     564 
   Size:         52      46      50     379      37

  Fragments arranged by size: 

                 379      52      50      46      37
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     564 
   Size:        295     269
 
MspA1I CmG'CkG

Cuts at:      0     140     519     564 
   Size:        140     379      45

  Fragments arranged by size: 

                 379     140      45
 
NdeI CA'TA_TG

Cuts at:      0     149     564 
   Size:        149     415
 
NspI r_CATG'y

Cuts at:      0     487     564 
   Size:        487      77
 
PasI CC'CwG_GG

Cuts at:      0      32     564 
   Size:         32     532
 
PvuII CAG'CTG

Cuts at:      0     140     519     564 
   Size:        140     379      45

  Fragments arranged by size: 

                 379     140      45
 
StuI AGG'CCT

Cuts at:      0      70     564 
   Size:         70     494
 
XmnI GAAnn'nnTTC

Cuts at:      0     363     564 
   Size:        363     201
 

 Enzymes that do cut:

     ApoI     AvaI     BcgI    BglII Bme1580I     BpmI     BsaI    BsaHI
    BsmBI Bsp1286I    BssSI    BstBI    BstYI   Bsu36I     BtgI     EarI
  Eco57MI   MspA1I     NdeI     NspI     PasI    PvuII     StuI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI   BstEII    BstXI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI