(Linear) (Six-base) MAPSORT of: KIBB7450.seq  Check: 2827  from: 1  to: 936



KIBB7450.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0     531     936 
   Size:        531     405
 
AflII C'TTAA_G

Cuts at:      0     381     936 
   Size:        381     555
 
AflIII A'CryG_T

Cuts at:      0     712     936 
   Size:        712     224
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     779     811     936 
   Size:        779      32     125

  Fragments arranged by size: 

                 779     125      32
 
BanII G_rGCy'C

Cuts at:      0       8     167     936 
   Size:          8     159     769

  Fragments arranged by size: 

                 769     159       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     735     936 
   Size:        735     201
 
BclI T'GATC_A

Cuts at:      0     147     936 
   Size:        147     789
 
BmtI G_CTAG'C

Cuts at:      0     556     936 
   Size:        556     380
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     936 
   Size:        704     232
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     643     936 
   Size:        643     293
 
BsaBI GATnn'nnATC

Cuts at:      0      76     936 
   Size:         76     860
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     701     936 
   Size:        701     235
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     586     936 
   Size:        586     350
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     167     936 
   Size:          8     159     769

  Fragments arranged by size: 

                 769     159       8
 
BsrDI GCAATG_nn'

Cuts at:      0     123     614     936 
   Size:        123     491     322

  Fragments arranged by size: 

                 491     322     123
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      37     936 
   Size:         37     899
 
BtgI C'CryG_G

Cuts at:      0     273     344     936 
   Size:        273      71     592

  Fragments arranged by size: 

                 592     273      71
 
EaeI y'GGCC_r

Cuts at:      0     270     936 
   Size:        270     666
 
EarI CTCTTCn'nnn_

Cuts at:      0     131     936 
   Size:        131     805
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     936 
   Size:        704     232
 
EcoO109I rG'GnC_Cy

Cuts at:      0     702     936 
   Size:        702     234
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     599     936 
   Size:        599     337
 
MscI TGG'CCA

Cuts at:      0     272     936 
   Size:        272     664
 
MslI CAynn'nnrTG

Cuts at:      0     539     817     936 
   Size:        539     278     119

  Fragments arranged by size: 

                 539     278     119
 
NcoI C'CATG_G

Cuts at:      0     273     344     936 
   Size:        273      71     592

  Fragments arranged by size: 

                 592     273      71
 
NheI G'CTAG_C

Cuts at:      0     552     936 
   Size:        552     384
 
NspI r_CATG'y

Cuts at:      0     716     796     936 
   Size:        716      80     140

  Fragments arranged by size: 

                 716     140      80
 
PciI A'CATG_T

Cuts at:      0     712     936 
   Size:        712     224
 
SfcI C'TryA_G

Cuts at:      0     197     755     867     936 
   Size:        197     558     112      69

  Fragments arranged by size: 

                 558     197     112      69
 
SmlI C'TyrA_G

Cuts at:      0     381     658     936 
   Size:        381     277     278

  Fragments arranged by size: 

                 381     278     277
 
StuI AGG'CCT

Cuts at:      0     295     936 
   Size:        295     641
 
StyI C'CwwG_G

Cuts at:      0     273     344     698     936 
   Size:        273      71     354     238

  Fragments arranged by size: 

                 354     273     238      71
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     424     936 
   Size:        424     512
 
TatI w'GTAC_w

Cuts at:      0     170     510     936 
   Size:        170     340     426

  Fragments arranged by size: 

                 426     340     170
 
XbaI T'CTAG_A

Cuts at:      0     928     936 
   Size:        928       8
 

 Enzymes that do cut:

     AccI    AflII   AflIII     AloI    BanII     BbsI     BclI     BmtI
     BpmI    BpuEI    BsaBI    BseRI    BsmBI Bsp1286I    BsrDI    BstXI
     BtgI     EaeI     EarI  Eco57MI EcoO109I     MmeI     MscI     MslI
     NcoI     NheI     NspI     PciI     SfcI     SmlI     StuI     StyI
    TaqII     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI