Restriction map for
pF1KB7451
(Linear) (Six-base) MAPSORT of: KIBB7451.seq Check: 3014 from: 1 to: 1248
KIBB7451.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AccI GT'mk_AC
Cuts at: 0 452 944 1248
Size: 452 492 304
Fragments arranged by size:
492 452 304
AflII C'TTAA_G
Cuts at: 0 1048 1248
Size: 1048 200
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 835 1248
Size: 835 413
ApoI r'AATT_y
Cuts at: 0 978 1248
Size: 978 270
AvaI C'yCGr_G
Cuts at: 0 540 1248
Size: 540 708
BamHI G'GATC_C
Cuts at: 0 603 1125 1248
Size: 603 522 123
Fragments arranged by size:
603 522 123
BanI G'GyrC_C
Cuts at: 0 9 205 1248
Size: 9 196 1043
Fragments arranged by size:
1043 196 9
BanII G_rGCy'C
Cuts at: 0 21 245 794 1248
Size: 21 224 549 454
Fragments arranged by size:
549 454 224 21
BbeI G_GCGC'C
Cuts at: 0 13 1248
Size: 13 1235
BbsI GAAGACnn'nnnn_
Cuts at: 0 146 1248
Size: 146 1102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 274 308 1248
Size: 274 34 940
Fragments arranged by size:
940 274 34
BlpI GC'TnA_GC
Cuts at: 0 244 1248
Size: 244 1004
Bme1580I G_kGCm'C
Cuts at: 0 824 1248
Size: 824 424
BmgBI CAC'GTC
Cuts at: 0 600 1248
Size: 600 648
BmrI ACTGGGnnnn_n'
Cuts at: 0 811 1248
Size: 811 437
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 343 1248
Size: 343 905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 772 1248
Size: 259 513 476
Fragments arranged by size:
513 476 259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 366 1248
Size: 123 243 882
Fragments arranged by size:
882 243 123
BsaHI Gr'CG_yC
Cuts at: 0 10 1116 1248
Size: 10 1106 132
Fragments arranged by size:
1106 132 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 135 718 721 724 727 1248
Size: 135 583 3 3 3 521
Fragments arranged by size:
583 521 135 3 3 3
BseYI C'CCAG_C
Cuts at: 0 281 651 1248
Size: 281 370 597
Fragments arranged by size:
597 370 281
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 309 1108 1248
Size: 60 249 799 140
Fragments arranged by size:
799 249 140 60
BsiEI CG_ry'CG
Cuts at: 0 200 1248
Size: 200 1048
BsiHKAI G_wGCw'C
Cuts at: 0 21 245 407 794 1248
Size: 21 224 162 387 454
Fragments arranged by size:
454 387 224 162 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 993 1248
Size: 993 255
Bsp1286I G_dGCh'C
Cuts at: 0 21 245 407 794 824 1248
Size: 21 224 162 387 30 424
Fragments arranged by size:
424 387 224 162 30 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 542 1248
Size: 542 706
BsrBI CCG'CTC
Cuts at: 0 1169 1248
Size: 1169 79
BsrFI r'CCGG_y
Cuts at: 0 202 208 1248
Size: 202 6 1040
Fragments arranged by size:
1040 202 6
BssSI C'ACGA_G
Cuts at: 0 14 1248
Size: 14 1234
BstAPI GCAn_nnn'nTGC
Cuts at: 0 471 1248
Size: 471 777
BstYI r'GATC_y
Cuts at: 0 603 1125 1248
Size: 603 522 123
Fragments arranged by size:
603 522 123
BtgI C'CryG_G
Cuts at: 0 669 1248
Size: 669 579
BtsI GCAGTG_nn'
Cuts at: 0 292 691 769 1248
Size: 292 399 78 479
Fragments arranged by size:
479 399 292 78
EaeI y'GGCC_r
Cuts at: 0 197 585 593 672 1248
Size: 197 388 8 79 576
Fragments arranged by size:
576 388 197 79 8
EagI C'GGCC_G
Cuts at: 0 197 1248
Size: 197 1051
EarI CTCTTCn'nnn_
Cuts at: 0 695 782 1248
Size: 695 87 466
Fragments arranged by size:
695 466 87
EcoICRI GAG'CTC
Cuts at: 0 19 243 792 1248
Size: 19 224 549 456
Fragments arranged by size:
549 456 224 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 772 1248
Size: 259 513 476
Fragments arranged by size:
513 476 259
EcoNI CCTnn'n_nnAGG
Cuts at: 0 501 1248
Size: 501 747
EcoO109I rG'GnC_Cy
Cuts at: 0 1105 1248
Size: 1105 143
HaeII r_GCGC'y
Cuts at: 0 13 233 1248
Size: 13 220 1015
Fragments arranged by size:
1015 220 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 1004 1036 1248
Size: 595 32 377 32 212
Fragments arranged by size:
595 377 212 32 32
HincII GTy'rAC
Cuts at: 0 614 1248
Size: 614 634
HindIII A'AGCT_T
Cuts at: 0 546 1248
Size: 546 702
KasI G'GCGC_C
Cuts at: 0 9 1248
Size: 9 1239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 577 1248
Size: 362 215 671
Fragments arranged by size:
671 362 215
MscI TGG'CCA
Cuts at: 0 587 674 1248
Size: 587 87 574
Fragments arranged by size:
587 574 87
MslI CAynn'nnrTG
Cuts at: 0 460 817 1248
Size: 460 357 431
Fragments arranged by size:
460 431 357
MspA1I CmG'CkG
Cuts at: 0 166 895 924 1248
Size: 166 729 29 324
Fragments arranged by size:
729 324 166 29
NaeI GCC'GGC
Cuts at: 0 204 1248
Size: 204 1044
NarI GG'CG_CC
Cuts at: 0 10 1248
Size: 10 1238
NcoI C'CATG_G
Cuts at: 0 669 1248
Size: 669 579
NgoMIV G'CCGG_C
Cuts at: 0 202 1248
Size: 202 1046
NotI GC'GGCC_GC
Cuts at: 0 197 1248
Size: 197 1051
NspI r_CATG'y
Cuts at: 0 1145 1248
Size: 1145 103
PasI CC'CwG_GG
Cuts at: 0 93 1248
Size: 93 1155
PflMI CCAn_nnn'nTGG
Cuts at: 0 750 1248
Size: 750 498
PfoI T'CCnGG_A
Cuts at: 0 329 1248
Size: 329 919
PpuMI rG'GwC_Cy
Cuts at: 0 1105 1248
Size: 1105 143
PstI C_TGCA'G
Cuts at: 0 863 1248
Size: 863 385
PvuII CAG'CTG
Cuts at: 0 895 924 1248
Size: 895 29 324
Fragments arranged by size:
895 324 29
SacI G_AGCT'C
Cuts at: 0 21 245 794 1248
Size: 21 224 549 454
Fragments arranged by size:
549 454 224 21
SapI GCTCTTCn'nnn_
Cuts at: 0 782 1248
Size: 782 466
ScaI AGT'ACT
Cuts at: 0 1054 1248
Size: 1054 194
SfcI C'TryA_G
Cuts at: 0 859 1248
Size: 859 389
SfoI GGC'GCC
Cuts at: 0 11 1248
Size: 11 1237
SgrAI Cr'CCGG_yG
Cuts at: 0 208 1248
Size: 208 1040
SmlI C'TyrA_G
Cuts at: 0 102 381 1048 1248
Size: 102 279 667 200
Fragments arranged by size:
667 279 200 102
StyI C'CwwG_G
Cuts at: 0 669 1065 1248
Size: 669 396 183
Fragments arranged by size:
669 396 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 692 1248
Size: 692 556
TatI w'GTAC_w
Cuts at: 0 1052 1248
Size: 1052 196
Enzymes that do cut:
AccI AflII AlfI ApoI AvaI BamHI BanI BanII
BbeI BbsI BcgI BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstYI
BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PflMI PfoI PpuMI PstI PvuII SacI SapI
ScaI SfcI SfoI SgrAI SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BglI
BglII BmtI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EciI EcoRI EcoRV FalI FseI FspI
FspAI HpaI KpnI MfeI MluI NdeI NheI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SgfI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI