Restriction map for
pF1KB7452
(Linear) (Six-base) MAPSORT of: KIBB7452.seq Check: 5306 from: 1 to: 813
KIBB7452.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 568 813
Size: 568 245
Acc65I G'GTAC_C
Cuts at: 0 30 813
Size: 30 783
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 813
Size: 178 635
AlwNI CAG_nnn'CTG
Cuts at: 0 273 291 612 813
Size: 273 18 321 201
Fragments arranged by size:
321 273 201 18
AvaI C'yCGr_G
Cuts at: 0 77 178 261 469 813
Size: 77 101 83 208 344
Fragments arranged by size:
344 208 101 83 77
BanI G'GyrC_C
Cuts at: 0 30 247 308 483 525 741 813
Size: 30 217 61 175 42 216 72
Fragments arranged by size:
217 216 175 72 61 42 30
BanII G_rGCy'C
Cuts at: 0 260 813
Size: 260 553
BbeI G_GCGC'C
Cuts at: 0 251 487 745 813
Size: 251 236 258 68
Fragments arranged by size:
258 251 236 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 531 813
Size: 531 282
BciVI GTATCCnnnnn_n'
Cuts at: 0 84 813
Size: 84 729
BglI GCCn_nnn'nGGC
Cuts at: 0 199 458 634 650 813
Size: 199 259 176 16 163
Fragments arranged by size:
259 199 176 163 16
BlpI GC'TnA_GC
Cuts at: 0 532 813
Size: 532 281
Bme1580I G_kGCm'C
Cuts at: 0 553 813
Size: 553 260
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 747 813
Size: 747 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 595 634 694 742 787 813
Size: 187 408 39 60 48 45 26
Fragments arranged by size:
408 187 60 48 45 39 26
Bpu10I CC'TnA_GC
Cuts at: 0 379 439 813
Size: 379 60 374
Fragments arranged by size:
379 374 60
BsaAI yAC'GTr
Cuts at: 0 145 813
Size: 145 668
BsaHI Gr'CG_yC
Cuts at: 0 248 484 698 742 813
Size: 248 236 214 44 71
Fragments arranged by size:
248 236 214 71 44
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 196 487 772 813
Size: 118 78 291 285 41
Fragments arranged by size:
291 285 118 78 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 813
Size: 523 290
BsiEI CG_ry'CG
Cuts at: 0 44 63 95 191 626 642 813
Size: 44 19 32 96 435 16 171
Fragments arranged by size:
435 171 96 44 32 19 16
Bsp1286I G_dGCh'C
Cuts at: 0 260 553 813
Size: 260 293 260
Fragments arranged by size:
293 260 260
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 111 568 813
Size: 111 457 245
Fragments arranged by size:
457 245 111
BsrFI r'CCGG_y
Cuts at: 0 39 43 385 592 727 804 813
Size: 39 4 342 207 135 77 9
Fragments arranged by size:
342 207 135 77 39 9 4
BssHII G'CGCG_C
Cuts at: 0 299 586 588 596 680 712 813
Size: 299 287 2 8 84 32 101
Fragments arranged by size:
299 287 101 84 32 8 2
BssSI C'ACGA_G
Cuts at: 0 517 813
Size: 517 296
BtgI C'CryG_G
Cuts at: 0 632 813
Size: 632 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 774 813
Size: 774 39
BtsI GCAGTG_nn'
Cuts at: 0 14 511 813
Size: 14 497 302
Fragments arranged by size:
497 302 14
EaeI y'GGCC_r
Cuts at: 0 41 60 92 459 623 635 813
Size: 41 19 32 367 164 12 178
Fragments arranged by size:
367 178 164 41 32 19 12
EagI C'GGCC_G
Cuts at: 0 41 60 92 623 813
Size: 41 19 32 531 190
Fragments arranged by size:
531 190 41 32 19
EarI CTCTTCn'nnn_
Cuts at: 0 394 607 790 813
Size: 394 213 183 23
Fragments arranged by size:
394 213 183 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 187 595 634 694 742 787 813
Size: 178 9 408 39 60 48 45 26
Fragments arranged by size:
408 178 60 48 45 39 26 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 118 157 813
Size: 118 39 656
Fragments arranged by size:
656 118 39
FspI TGC'GCA
Cuts at: 0 496 813
Size: 496 317
HaeII r_GCGC'y
Cuts at: 0 251 291 386 487 745 813
Size: 251 40 95 101 258 68
Fragments arranged by size:
258 251 101 95 68 40
KasI G'GCGC_C
Cuts at: 0 247 483 741 813
Size: 247 236 258 72
Fragments arranged by size:
258 247 236 72
KpnI G_GTAC'C
Cuts at: 0 34 813
Size: 34 779
MspA1I CmG'CkG
Cuts at: 0 59 489 531 609 813
Size: 59 430 42 78 204
Fragments arranged by size:
430 204 78 59 42
NaeI GCC'GGC
Cuts at: 0 41 387 594 729 813
Size: 41 346 207 135 84
Fragments arranged by size:
346 207 135 84 41
NarI GG'CG_CC
Cuts at: 0 248 484 742 813
Size: 248 236 258 71
Fragments arranged by size:
258 248 236 71
NgoMIV G'CCGG_C
Cuts at: 0 39 385 592 727 813
Size: 39 346 207 135 86
Fragments arranged by size:
346 207 135 86 39
NotI GC'GGCC_GC
Cuts at: 0 60 813
Size: 60 753
NruI TCG'CGA
Cuts at: 0 793 813
Size: 793 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 612 813
Size: 612 201
PfoI T'CCnGG_A
Cuts at: 0 719 813
Size: 719 94
PstI C_TGCA'G
Cuts at: 0 281 689 813
Size: 281 408 124
Fragments arranged by size:
408 281 124
PvuI CG_AT'CG
Cuts at: 0 191 813
Size: 191 622
PvuII CAG'CTG
Cuts at: 0 609 813
Size: 609 204
SapI GCTCTTCn'nnn_
Cuts at: 0 607 790 813
Size: 607 183 23
Fragments arranged by size:
607 183 23
SfcI C'TryA_G
Cuts at: 0 277 426 685 813
Size: 277 149 259 128
Fragments arranged by size:
277 259 149 128
SfoI GGC'GCC
Cuts at: 0 249 485 743 813
Size: 249 236 258 70
Fragments arranged by size:
258 249 236 70
SgrAI Cr'CCGG_yG
Cuts at: 0 385 592 813
Size: 385 207 221
Fragments arranged by size:
385 221 207
SmaI CCC'GGG
Cuts at: 0 79 263 813
Size: 79 184 550
Fragments arranged by size:
550 184 79
XmaI C'CCGG_G
Cuts at: 0 77 261 813
Size: 77 184 552
Fragments arranged by size:
552 184 77
Enzymes that do cut:
AarI Acc65I AcuI AlwNI AvaI BanI BanII BbeI
BbsI BciVI BglI BlpI Bme1580I BplI BpmI Bpu10I
BsaAI BsaHI BseRI BsgI BsiEI Bsp1286I BspMI BsrFI
BssHII BssSI BtgI BtgZI BtsI EaeI EagI EarI
Eco57MI EcoNI FspI HaeII KasI KpnI MspA1I NaeI
NarI NgoMIV NotI NruI PflMI PfoI PstI PvuI
PvuII SapI SfcI SfoI SgrAI SmaI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BclI BfrBI
BglII BmgBI BmrI BmtI BpuEI BsaI BsaBI BsaWI
BsaXI BseYI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI
EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspAI Hin4I
HincII HindIII HpaI MfeI MluI MmeI MscI MslI
NcoI NdeI NheI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI