(Linear) (Six-base) MAPSORT of: KIBB7501.seq Check: 4329 from: 1 to: 174
KIBB7501.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
BamHI G'GATC_C
Cuts at: 0 93 174
Size: 93 81
BciVI GTATCCnnnnn_n'
Cuts at: 0 112 174
Size: 112 62
BsaBI GATnn'nnATC
Cuts at: 0 49 174
Size: 49 125
BsrGI T'GTAC_A
Cuts at: 0 131 174
Size: 131 43
BstYI r'GATC_y
Cuts at: 0 93 174
Size: 93 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 22 174
Size: 22 152
TatI w'GTAC_w
Cuts at: 0 131 174
Size: 131 43
Enzymes that do cut:
BamHI BciVI BsaBI BsrGI BstYI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI