(Linear) (Six-base) MAPSORT of: KIBB7501.seq Check: 4329 from: 1 to: 174 KIBB7501.orf With 182 enzymes: SgfI * March 3, 2008 11:01 .. BamHI G'GATC_C Cuts at: 0 93 174 Size: 93 81 BciVI GTATCCnnnnn_n' Cuts at: 0 112 174 Size: 112 62 BsaBI GATnn'nnATC Cuts at: 0 49 174 Size: 49 125 BsrGI T'GTAC_A Cuts at: 0 131 174 Size: 131 43 BstYI r'GATC_y Cuts at: 0 93 174 Size: 93 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 22 174 Size: 22 152 TatI w'GTAC_w Cuts at: 0 131 174 Size: 131 43 Enzymes that do cut: BamHI BciVI BsaBI BsrGI BstYI TaqII TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI