(Linear) (Six-base) MAPSORT of: KIBB7502.seq  Check: 9763  from: 1  to: 708



KIBB7502.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AseI AT'TA_AT

Cuts at:      0     527     708 
   Size:        527     181
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      16      49     708 
   Size:         16      33     659

  Fragments arranged by size: 

                 659      33      16
 
BanI G'GyrC_C

Cuts at:      0     354     708 
   Size:        354     354
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     178     708 
   Size:        178     530
 
BbvCI CC'TCA_GC

Cuts at:      0     503     708 
   Size:        503     205
 
BclI T'GATC_A

Cuts at:      0     390     708 
   Size:        390     318
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     708 
   Size:        383     325
 
Bpu10I CC'TnA_GC

Cuts at:      0     503     708 
   Size:        503     205
 
BsaBI GATnn'nnATC

Cuts at:      0     389     512     708 
   Size:        389     123     196

  Fragments arranged by size: 

                 389     196     123
 
BsaHI Gr'CG_yC

Cuts at:      0       4     708 
   Size:          4     704
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     194     224     356     386     708 
   Size:        194      30     132      30     322

  Fragments arranged by size: 

                 322     194     132      30      30
 
BseYI C'CCAG_C

Cuts at:      0      93     708 
   Size:         93     615
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     522     708 
   Size:        522     186
 
BspHI T'CATG_A

Cuts at:      0     141     456     708 
   Size:        141     315     252

  Fragments arranged by size: 

                 315     252     141
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     553     708 
   Size:        553     155
 
BtgI C'CryG_G

Cuts at:      0     547     708 
   Size:        547     161
 
BtsI GCAGTG_nn'

Cuts at:      0     222     708 
   Size:        222     486
 
EarI CTCTTCn'nnn_

Cuts at:      0     161     610     708 
   Size:        161     449      98

  Fragments arranged by size: 

                 449     161      98
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     708 
   Size:        383     325
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     502     708 
   Size:        502     206
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     639     708 
   Size:        639      69
 
HaeII r_GCGC'y

Cuts at:      0     471     708 
   Size:        471     237
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     180     708 
   Size:        180     528
 
PstI C_TGCA'G

Cuts at:      0     564     708 
   Size:        564     144
 
SfcI C'TryA_G

Cuts at:      0     560     708 
   Size:        560     148
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     194     226     356     388     708 
   Size:        194      32     130      32     320

  Fragments arranged by size: 

                 320     194     130      32      32
 

 Enzymes that do cut:

     AseI     BaeI     BanI     BbsI    BbvCI     BclI     BpmI   Bpu10I
    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BspHI    BspMI     BtgI
     BtsI     EarI  Eco57MI    EcoNI     FalI    HaeII     MmeI     PstI
     SfcI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI    AsiSI     AvaI    AvrII    BamHI    BanII
     BbeI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaWI
    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI