(Linear) (Six-base) MAPSORT of: KIBB7503.seq  Check: 6721  from: 1  to: 228



KIBB7503.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AleI CACnn'nnGTG

Cuts at:      0      40     228 
   Size:         40     188
 
ApoI r'AATT_y

Cuts at:      0      76     228 
   Size:         76     152
 
BseYI C'CCAG_C

Cuts at:      0     175     228 
   Size:        175      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46      67     228 
   Size:         46      21     161

  Fragments arranged by size: 

                 161      46      21
 
BspHI T'CATG_A

Cuts at:      0     164     228 
   Size:        164      64
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     219     228 
   Size:        219       9
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      66     228 
   Size:         66     162
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     103     228 
   Size:        103     125
 
EaeI y'GGCC_r

Cuts at:      0       2     228 
   Size:          2     226
 
EcoO109I rG'GnC_Cy

Cuts at:      0      30     228 
   Size:         30     198
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      22      54     228 
   Size:         22      32     174

  Fragments arranged by size: 

                 174      32      22
 
MslI CAynn'nnrTG

Cuts at:      0      40     228 
   Size:         40     188
 
PpuMI rG'GwC_Cy

Cuts at:      0      30     228 
   Size:         30     198
 
PstI C_TGCA'G

Cuts at:      0      62     228 
   Size:         62     166
 
SfcI C'TryA_G

Cuts at:      0      58     228 
   Size:         58     170
 
Tth111I GACn'n_nGTC

Cuts at:      0      87     228 
   Size:         87     141
 

 Enzymes that do cut:

     AleI     ApoI    BseYI     BsgI    BspHI    BspMI   BstAPI    BstXI
     EaeI EcoO109I    Hin4I     MslI    PpuMI     PstI     SfcI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI