(Linear) (Six-base) MAPSORT of: KIBB7505.seq  Check: 3531  from: 1  to: 294



KIBB7505.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0     194     294 
   Size:        194     100
 
AlwNI CAG_nnn'CTG

Cuts at:      0      21     294 
   Size:         21     273
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     140     294 
   Size:        140     154
 
BclI T'GATC_A

Cuts at:      0     116     294 
   Size:        116     178
 
BlpI GC'TnA_GC

Cuts at:      0       4     232     294 
   Size:          4     228      62

  Fragments arranged by size: 

                 228      62       4
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     294 
   Size:        247      47
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     294 
   Size:         70     224
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46      49     274     277     280     294 
   Size:         46       3     225       3       3      14

  Fragments arranged by size: 

                 225      46      14       3       3       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     294 
   Size:         49     245
 
BsiHKAI G_wGCw'C

Cuts at:      0     238     294 
   Size:        238      56
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      31     294 
   Size:         31     263
 
Bsp1286I G_dGCh'C

Cuts at:      0     238     294 
   Size:        238      56
 
BsrFI r'CCGG_y

Cuts at:      0       8     294 
   Size:          8     286
 
EarI CTCTTCn'nnn_

Cuts at:      0     123     251     266     294 
   Size:        123     128      15      28

  Fragments arranged by size: 

                 128     123      28      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     294 
   Size:        247      47
 
FspI TGC'GCA

Cuts at:      0      25     294 
   Size:         25     269
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     294 
   Size:        130      32     132

  Fragments arranged by size: 

                 132     130      32
 
MspA1I CmG'CkG

Cuts at:      0      75     294 
   Size:         75     219
 
NaeI GCC'GGC

Cuts at:      0      10     294 
   Size:         10     284
 
NgoMIV G'CCGG_C

Cuts at:      0       8     294 
   Size:          8     286
 
NspI r_CATG'y

Cuts at:      0     193     294 
   Size:        193     101
 
PvuII CAG'CTG

Cuts at:      0      75     294 
   Size:         75     219
 
SmlI C'TyrA_G

Cuts at:      0      85     294 
   Size:         85     209
 
XbaI T'CTAG_A

Cuts at:      0     124     294 
   Size:        124     170
 

 Enzymes that do cut:

     AccI    AlwNI     BbsI     BclI     BlpI     BpmI    BpuEI    BseRI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BsrFI     EarI  Eco57MI     FspI
    Hin4I   MspA1I     NaeI   NgoMIV     NspI    PvuII     SmlI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI