(Linear) (Six-base) MAPSORT of: KIBB7507.seq  Check: 8190  from: 1  to: 327



KIBB7507.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28      97     327 
   Size:         28      69     230

  Fragments arranged by size: 

                 230      69      28
 
AlwNI CAG_nnn'CTG

Cuts at:      0     200     327 
   Size:        200     127
 
ApoI r'AATT_y

Cuts at:      0     238     327 
   Size:        238      89
 
BanII G_rGCy'C

Cuts at:      0      65     181     327 
   Size:         65     116     146

  Fragments arranged by size: 

                 146     116      65
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      34     327 
   Size:         34     293
 
BsaAI yAC'GTr

Cuts at:      0     191     327 
   Size:        191     136
 
BsiHKAI G_wGCw'C

Cuts at:      0      65     327 
   Size:         65     262
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     181     327 
   Size:         65     116     146

  Fragments arranged by size: 

                 146     116      65
 
EcoICRI GAG'CTC

Cuts at:      0      63     327 
   Size:         63     264
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28      97     327 
   Size:         28      69     230

  Fragments arranged by size: 

                 230      69      28
 
EcoRI G'AATT_C

Cuts at:      0     238     327 
   Size:        238      89
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     127     327 
   Size:        127     200
 
SacI G_AGCT'C

Cuts at:      0      65     327 
   Size:         65     262
 
SfcI C'TryA_G

Cuts at:      0     320     327 
   Size:        320       7
 
SnaBI TAC'GTA

Cuts at:      0     191     327 
   Size:        191     136
 
TatI w'GTAC_w

Cuts at:      0     222     327 
   Size:        222     105
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BanII     BbsI    BsaAI  BsiHKAI Bsp1286I
  EcoICRI  Eco57MI    EcoRI     FalI     SacI     SfcI    SnaBI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI