(Linear) (Six-base) MAPSORT of: KIBB7509.seq  Check: 553  from: 1  to: 336



KIBB7509.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AflIII A'CryG_T

Cuts at:      0     201     336 
   Size:        201     135
 
ApoI r'AATT_y

Cuts at:      0      82     274     336 
   Size:         82     192      62

  Fragments arranged by size: 

                 192      82      62
 
BfrBI ATG'CAT

Cuts at:      0     106     221     336 
   Size:        106     115     115

  Fragments arranged by size: 

                 115     115     106
 
BsaAI yAC'GTr

Cuts at:      0     233     336 
   Size:        233     103
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     336 
   Size:         25     311
 
EaeI y'GGCC_r

Cuts at:      0      75     336 
   Size:         75     261
 
EcoO109I rG'GnC_Cy

Cuts at:      0      37     336 
   Size:         37     299
 
EcoRI G'AATT_C

Cuts at:      0     274     336 
   Size:        274      62
 
EcoRV GAT'ATC

Cuts at:      0      65     336 
   Size:         65     271
 
MfeI C'AATT_G

Cuts at:      0     281     336 
   Size:        281      55
 
MscI TGG'CCA

Cuts at:      0      77     336 
   Size:         77     259
 
NsiI A_TGCA'T

Cuts at:      0     108     223     336 
   Size:        108     115     113

  Fragments arranged by size: 

                 115     113     108
 
NspI r_CATG'y

Cuts at:      0     106     205     225     336 
   Size:        106      99      20     111

  Fragments arranged by size: 

                 111     106      99      20
 
PciI A'CATG_T

Cuts at:      0     201     336 
   Size:        201     135
 
PpuMI rG'GwC_Cy

Cuts at:      0      37     336 
   Size:         37     299
 
SnaBI TAC'GTA

Cuts at:      0     233     336 
   Size:        233     103
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     252     284     336 
   Size:        252      32      52

  Fragments arranged by size: 

                 252      52      32
 

 Enzymes that do cut:

   AflIII     ApoI    BfrBI    BsaAI    BseRI     EaeI EcoO109I    EcoRI
    EcoRV     MfeI     MscI     NsiI     NspI     PciI    PpuMI    SnaBI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI