Restriction map for
pF1KB7510
(Linear) (Six-base) MAPSORT of: KIBB7510.seq Check: 919 from: 1 to: 726
KIBB7510.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
Acc65I G'GTAC_C
Cuts at: 0 277 726
Size: 277 449
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 358 726
Size: 24 334 368
Fragments arranged by size:
368 334 24
AlwNI CAG_nnn'CTG
Cuts at: 0 126 726
Size: 126 600
ApoI r'AATT_y
Cuts at: 0 472 726
Size: 472 254
BanI G'GyrC_C
Cuts at: 0 73 277 726
Size: 73 204 449
Fragments arranged by size:
449 204 73
BbeI G_GCGC'C
Cuts at: 0 77 726
Size: 77 649
BbsI GAAGACnn'nnnn_
Cuts at: 0 41 726
Size: 41 685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 511 545 726
Size: 511 34 181
Fragments arranged by size:
511 181 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 383 521 694 726
Size: 136 247 138 173 32
Fragments arranged by size:
247 173 138 136 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 726
Size: 703 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 639 726
Size: 639 87
BsaHI Gr'CG_yC
Cuts at: 0 74 726
Size: 74 652
BseYI C'CCAG_C
Cuts at: 0 295 726
Size: 295 431
BsiHKAI G_wGCw'C
Cuts at: 0 101 726
Size: 101 625
BsmI GAATG_Cn'
Cuts at: 0 594 726
Size: 594 132
Bsp1286I G_dGCh'C
Cuts at: 0 101 726
Size: 101 625
BspHI T'CATG_A
Cuts at: 0 252 726
Size: 252 474
BsrDI GCAATG_nn'
Cuts at: 0 632 726
Size: 632 94
BsrFI r'CCGG_y
Cuts at: 0 88 726
Size: 88 638
BstAPI GCAn_nnn'nTGC
Cuts at: 0 600 726
Size: 600 126
BstEII G'GTnAC_C
Cuts at: 0 609 726
Size: 609 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 542 581 726
Size: 542 39 145
Fragments arranged by size:
542 145 39
BtgI C'CryG_G
Cuts at: 0 614 726
Size: 614 112
EaeI y'GGCC_r
Cuts at: 0 2 150 726
Size: 2 148 576
Fragments arranged by size:
576 148 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 316 726
Size: 316 410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 136 358 383 521 694 726
Size: 24 112 222 25 138 173 32
Fragments arranged by size:
222 173 138 112 32 25 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 118 726
Size: 118 608
EcoO109I rG'GnC_Cy
Cuts at: 0 123 726
Size: 123 603
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 726
Size: 183 543
HaeII r_GCGC'y
Cuts at: 0 77 726
Size: 77 649
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 469 501 726
Size: 220 32 217 32 225
Fragments arranged by size:
225 220 217 32 32
HincII GTy'rAC
Cuts at: 0 321 726
Size: 321 405
HindIII A'AGCT_T
Cuts at: 0 25 179 726
Size: 25 154 547
Fragments arranged by size:
547 154 25
KasI G'GCGC_C
Cuts at: 0 73 726
Size: 73 653
KpnI G_GTAC'C
Cuts at: 0 281 726
Size: 281 445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 240 726
Size: 203 37 486
Fragments arranged by size:
486 203 37
MspA1I CmG'CkG
Cuts at: 0 80 726
Size: 80 646
NarI GG'CG_CC
Cuts at: 0 74 726
Size: 74 652
PflMI CCAn_nnn'nTGG
Cuts at: 0 696 726
Size: 696 30
PfoI T'CCnGG_A
Cuts at: 0 113 726
Size: 113 613
PsiI TTA'TAA
Cuts at: 0 185 726
Size: 185 541
PstI C_TGCA'G
Cuts at: 0 348 726
Size: 348 378
SfcI C'TryA_G
Cuts at: 0 344 726
Size: 344 382
SfoI GGC'GCC
Cuts at: 0 75 726
Size: 75 651
SmlI C'TyrA_G
Cuts at: 0 718 726
Size: 718 8
StuI AGG'CCT
Cuts at: 0 498 726
Size: 498 228
StyI C'CwwG_G
Cuts at: 0 455 726
Size: 455 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 338 726
Size: 338 388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 693 726
Size: 693 33
Enzymes that do cut:
Acc65I AcuI AlwNI ApoI BanI BbeI BbsI BcgI
BpmI BpuEI BsaI BsaHI BseYI BsiHKAI BsmI Bsp1286I
BspHI BsrDI BsrFI BstAPI BstEII BstXI BtgI EaeI
EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MspA1I NarI PflMI PfoI
PsiI PstI SfcI SfoI SmlI StuI StyI TaqII
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsaXI BseRI
BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrGI
BssHII BssSI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI
EcoRI EcoRV FseI FspI FspAI HpaI MfeI MluI
MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI