(Linear) (Six-base) MAPSORT of: KIBB7511.seq  Check: 6228  from: 1  to: 375



KIBB7511.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18     256     375 
   Size:         18     238     119

  Fragments arranged by size: 

                 238     119      18
 
AleI CACnn'nnGTG

Cuts at:      0     250     375 
   Size:        250     125
 
AvaI C'yCGr_G

Cuts at:      0     310     375 
   Size:        310      65
 
BanII G_rGCy'C

Cuts at:      0     272     375 
   Size:        272     103
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     182     375 
   Size:        182     193
 
BmgBI CAC'GTC

Cuts at:      0     248     375 
   Size:        248     127
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      12     187     202     375 
   Size:         12     175      15     173

  Fragments arranged by size: 

                 175     173      15      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      35     108     375 
   Size:         35      73     267

  Fragments arranged by size: 

                 267      73      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     375 
   Size:        145     230
 
BsiHKAI G_wGCw'C

Cuts at:      0     280     375 
   Size:        280      95
 
BsmI GAATG_Cn'

Cuts at:      0     334     375 
   Size:        334      41
 
Bsp1286I G_dGCh'C

Cuts at:      0     272     280     375 
   Size:        272       8      95

  Fragments arranged by size: 

                 272      95       8
 
BsrBI CCG'CTC

Cuts at:      0      41     375 
   Size:         41     334
 
BsrGI T'GTAC_A

Cuts at:      0     320     375 
   Size:        320      55
 
BssSI C'ACGA_G

Cuts at:      0     265     375 
   Size:        265     110
 
BtsI GCAGTG_nn'

Cuts at:      0      27     375 
   Size:         27     348
 
EaeI y'GGCC_r

Cuts at:      0     287     375 
   Size:        287      88
 
EarI CTCTTCn'nnn_

Cuts at:      0      78     118     375 
   Size:         78      40     257

  Fragments arranged by size: 

                 257      78      40
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      12      18     187     202     256     375 
   Size:         12       6     169      15      54     119

  Fragments arranged by size: 

                 169     119      54      15      12       6
 
HaeII r_GCGC'y

Cuts at:      0     329     375 
   Size:        329      46
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     375 
   Size:         38     337
 
MslI CAynn'nnrTG

Cuts at:      0     250     375 
   Size:        250     125
 
SapI GCTCTTCn'nnn_

Cuts at:      0     118     375 
   Size:        118     257
 
ScaI AGT'ACT

Cuts at:      0     241     375 
   Size:        241     134
 
TatI w'GTAC_w

Cuts at:      0     239     320     375 
   Size:        239      81      55

  Fragments arranged by size: 

                 239      81      55
 
XmnI GAAnn'nnTTC

Cuts at:      0     241     375 
   Size:        241     134
 

 Enzymes that do cut:

     AcuI     AleI     AvaI    BanII     BbsI    BmgBI     BpmI    BseRI
     BsgI  BsiHKAI     BsmI Bsp1286I    BsrBI    BsrGI    BssSI     BtsI
     EaeI     EarI  Eco57MI    HaeII     MmeI     MslI     SapI     ScaI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrDI    BsrFI   BssHII   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI