(Linear) (Six-base) MAPSORT of: KIBB7512.seq  Check: 778  from: 1  to: 729



KIBB7512.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AleI CACnn'nnGTG

Cuts at:      0     311     729 
   Size:        311     418
 
AlwNI CAG_nnn'CTG

Cuts at:      0     202     532     663     729 
   Size:        202     330     131      66

  Fragments arranged by size: 

                 330     202     131      66
 
AvaI C'yCGr_G

Cuts at:      0     278     729 
   Size:        278     451
 
BanI G'GyrC_C

Cuts at:      0     668     729 
   Size:        668      61
 
BbvCI CC'TCA_GC

Cuts at:      0     478     729 
   Size:        478     251
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     520     729 
   Size:        520     209
 
Bpu10I CC'TnA_GC

Cuts at:      0     478     656     729 
   Size:        478     178      73

  Fragments arranged by size: 

                 478     178      73
 
BsaBI GATnn'nnATC

Cuts at:      0     119     729 
   Size:        119     610
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     345     415     418     433     729 
   Size:        345      70       3      15     296

  Fragments arranged by size: 

                 345     296      70      15       3
 
BsiHKAI G_wGCw'C

Cuts at:      0      95     729 
   Size:         95     634
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     729 
   Size:         95     634
 
BspHI T'CATG_A

Cuts at:      0     191     729 
   Size:        191     538
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      46     729 
   Size:         46     683
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     313     729 
   Size:        313     416
 
BstYI r'GATC_y

Cuts at:      0     120     729 
   Size:        120     609
 
BtgI C'CryG_G

Cuts at:      0     124     729 
   Size:        124     605
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     446     729 
   Size:        446     283
 
BtsI GCAGTG_nn'

Cuts at:      0     318     729 
   Size:        318     411
 
ClaI AT'CG_AT

Cuts at:      0     113     729 
   Size:        113     616
 
EaeI y'GGCC_r

Cuts at:      0      77     729 
   Size:         77     652
 
EarI CTCTTCn'nnn_

Cuts at:      0     363     500     634     729 
   Size:        363     137     134      95

  Fragments arranged by size: 

                 363     137     134      95
 
EcoO109I rG'GnC_Cy

Cuts at:      0     660     729 
   Size:        660      69
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     205     237     729 
   Size:        205      32     492

  Fragments arranged by size: 

                 492     205      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     404     729 
   Size:        403       1     325

  Fragments arranged by size: 

                 403     325       1
 
MslI CAynn'nnrTG

Cuts at:      0     311     490     624     729 
   Size:        311     179     134     105

  Fragments arranged by size: 

                 311     179     134     105
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     532     729 
   Size:        532     197
 
PfoI T'CCnGG_A

Cuts at:      0     439     531     729 
   Size:        439      92     198

  Fragments arranged by size: 

                 439     198      92
 
SmlI C'TyrA_G

Cuts at:      0     278     729 
   Size:        278     451
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     357     729 
   Size:        357     372
 
Tth111I GACn'n_nGTC

Cuts at:      0     261     729 
   Size:        261     468
 
XhoI C'TCGA_G

Cuts at:      0     278     729 
   Size:        278     451
 

 Enzymes that do cut:

     AleI    AlwNI     AvaI     BanI    BbvCI     BmrI   Bpu10I    BsaBI
    BseRI  BsiHKAI Bsp1286I    BspHI    BspMI    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI     EaeI     EarI EcoO109I    Hin4I     MmeI
     MslI    PflMI     PfoI     SmlI    TaqII  Tth111I     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI
     XbaI     XcmI     XmaI     XmnI     ZraI