(Linear) (Six-base) MAPSORT of: KIBB7513.seq  Check: 9866  from: 1  to: 375



KIBB7513.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AflIII A'CryG_T

Cuts at:      0     110     212     335     375 
   Size:        110     102     123      40

  Fragments arranged by size: 

                 123     110     102      40
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     268     300     375 
   Size:        268      32      75

  Fragments arranged by size: 

                 268      75      32
 
ApaLI G'TGCA_C

Cuts at:      0     339     375 
   Size:        339      36
 
BanII G_rGCy'C

Cuts at:      0       8     375 
   Size:          8     367
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      89     375 
   Size:         89     286
 
Bme1580I G_kGCm'C

Cuts at:      0     343     375 
   Size:        343      32
 
BmgBI CAC'GTC

Cuts at:      0     213     375 
   Size:        213     162
 
BsaAI yAC'GTr

Cuts at:      0     336     375 
   Size:        336      39
 
BsaHI Gr'CG_yC

Cuts at:      0     317     375 
   Size:        317      58
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     268     298     375 
   Size:        268      30      77

  Fragments arranged by size: 

                 268      77      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     343     375 
   Size:        343      32
 
BsmI GAATG_Cn'

Cuts at:      0      98     375 
   Size:         98     277
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     343     375 
   Size:          8     335      32

  Fragments arranged by size: 

                 335      32       8
 
BtgI C'CryG_G

Cuts at:      0     235     375 
   Size:        235     140
 
BtsI GCAGTG_nn'

Cuts at:      0     351     375 
   Size:        351      24
 
DraIII CAC_nnn'GTG

Cuts at:      0     183     301     375 
   Size:        183     118      74

  Fragments arranged by size: 

                 183     118      74
 
EaeI y'GGCC_r

Cuts at:      0     267     357     375 
   Size:        267      90      18

  Fragments arranged by size: 

                 267      90      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0     222     375 
   Size:        222     153
 
HincII GTy'rAC

Cuts at:      0     165     375 
   Size:        165     210
 
MluI A'CGCG_T

Cuts at:      0     110     375 
   Size:        110     265
 
MspA1I CmG'CkG

Cuts at:      0     152     237     365     375 
   Size:        152      85     128      10

  Fragments arranged by size: 

                 152     128      85      10
 
NspI r_CATG'y

Cuts at:      0      69     375 
   Size:         69     306
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     268     300     375 
   Size:        268      32      75

  Fragments arranged by size: 

                 268      75      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     222     375 
   Size:        222     153
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     112     144     375 
   Size:        112      32     231

  Fragments arranged by size: 

                 231     112      32
 
PvuII CAG'CTG

Cuts at:      0     152     375 
   Size:        152     223
 
RsrII CG'GwC_CG

Cuts at:      0     239     375 
   Size:        239     136
 
SacII CC_GC'GG

Cuts at:      0     238     375 
   Size:        238     137
 
StyI C'CwwG_G

Cuts at:      0      77     375 
   Size:         77     298
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     256     375 
   Size:        256     119
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     238     270     375 
   Size:        238      32     105

  Fragments arranged by size: 

                 238     105      32
 

 Enzymes that do cut:

   AflIII     AloI    ApaLI    BanII     BbsI Bme1580I    BmgBI    BsaAI
    BsaHI    BsaXI  BsiHKAI     BsmI Bsp1286I     BtgI     BtsI   DraIII
     EaeI EcoO109I   HincII     MluI   MspA1I     NspI     PpiI    PpuMI
     PsrI    PvuII    RsrII    SacII     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaWI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI