Restriction map for
pF1KB7514
(Linear) (Six-base) MAPSORT of: KIBB7514.seq Check: 7630 from: 1 to: 735
KIBB7514.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 691 735
Size: 691 44
AlwNI CAG_nnn'CTG
Cuts at: 0 193 453 719 735
Size: 193 260 266 16
Fragments arranged by size:
266 260 193 16
ApaI G_GGCC'C
Cuts at: 0 19 169 503 735
Size: 19 150 334 232
Fragments arranged by size:
334 232 150 19
AvaI C'yCGr_G
Cuts at: 0 371 735
Size: 371 364
BanII G_rGCy'C
Cuts at: 0 19 169 444 503 735
Size: 19 150 275 59 232
Fragments arranged by size:
275 232 150 59 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 640 735
Size: 640 95
BbvCI CC'TCA_GC
Cuts at: 0 8 735
Size: 8 727
BfrBI ATG'CAT
Cuts at: 0 683 735
Size: 683 52
BglI GCCn_nnn'nGGC
Cuts at: 0 469 735
Size: 469 266
BglII A'GATC_T
Cuts at: 0 296 380 735
Size: 296 84 355
Fragments arranged by size:
355 296 84
BlpI GC'TnA_GC
Cuts at: 0 79 735
Size: 79 656
Bme1580I G_kGCm'C
Cuts at: 0 19 169 503 735
Size: 19 150 334 232
Fragments arranged by size:
334 232 150 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 735
Size: 310 425
Bpu10I CC'TnA_GC
Cuts at: 0 8 735
Size: 8 727
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 735
Size: 548 187
BsaAI yAC'GTr
Cuts at: 0 506 735
Size: 506 229
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 240 270 735
Size: 240 30 465
Fragments arranged by size:
465 240 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 141 527 598 735
Size: 141 386 71 137
Fragments arranged by size:
386 141 137 71
BseYI C'CCAG_C
Cuts at: 0 496 612 735
Size: 496 116 123
Fragments arranged by size:
496 123 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 735
Size: 397 338
Bsp1286I G_dGCh'C
Cuts at: 0 19 169 444 503 735
Size: 19 150 275 59 232
Fragments arranged by size:
275 232 150 59 19
BssSI C'ACGA_G
Cuts at: 0 543 735
Size: 543 192
BstXI CCAn_nnnn'nTGG
Cuts at: 0 486 735
Size: 486 249
BstYI r'GATC_y
Cuts at: 0 296 380 735
Size: 296 84 355
Fragments arranged by size:
355 296 84
Bsu36I CC'TnA_GG
Cuts at: 0 186 735
Size: 186 549
BtgI C'CryG_G
Cuts at: 0 622 735
Size: 622 113
BtsI GCAGTG_nn'
Cuts at: 0 515 735
Size: 515 220
EarI CTCTTCn'nnn_
Cuts at: 0 155 232 735
Size: 155 77 503
Fragments arranged by size:
503 155 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 426 735
Size: 426 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 735
Size: 310 425
EcoNI CCTnn'n_nnAGG
Cuts at: 0 641 735
Size: 641 94
EcoO109I rG'GnC_Cy
Cuts at: 0 16 66 165 166 735
Size: 16 50 99 1 569
Fragments arranged by size:
569 99 50 16 1
FspI TGC'GCA
Cuts at: 0 526 735
Size: 526 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 537 569 735
Size: 537 32 166
Fragments arranged by size:
537 166 32
MslI CAynn'nnrTG
Cuts at: 0 673 735
Size: 673 62
MspA1I CmG'CkG
Cuts at: 0 279 342 453 573 735
Size: 279 63 111 120 162
Fragments arranged by size:
279 162 120 111 63
NcoI C'CATG_G
Cuts at: 0 622 735
Size: 622 113
NsiI A_TGCA'T
Cuts at: 0 685 735
Size: 685 50
PmlI CAC'GTG
Cuts at: 0 506 735
Size: 506 229
PspOMI G'GGCC_C
Cuts at: 0 15 165 499 735
Size: 15 150 334 236
Fragments arranged by size:
334 236 150 15
PstI C_TGCA'G
Cuts at: 0 31 735
Size: 31 704
PvuII CAG'CTG
Cuts at: 0 279 342 453 573 735
Size: 279 63 111 120 162
Fragments arranged by size:
279 162 120 111 63
SapI GCTCTTCn'nnn_
Cuts at: 0 232 735
Size: 232 503
SfcI C'TryA_G
Cuts at: 0 27 735
Size: 27 708
SmlI C'TyrA_G
Cuts at: 0 563 735
Size: 563 172
StuI AGG'CCT
Cuts at: 0 117 735
Size: 117 618
StyI C'CwwG_G
Cuts at: 0 622 735
Size: 622 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 635 735
Size: 635 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 623 655 735
Size: 623 32 80
Fragments arranged by size:
623 80 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 620 704 735
Size: 620 84 31
Fragments arranged by size:
620 84 31
XmnI GAAnn'nnTTC
Cuts at: 0 298 735
Size: 298 437
Enzymes that do cut:
AlfI AlwNI ApaI AvaI BanII BbsI BbvCI BfrBI
BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI
BsaXI BseRI BseYI BsgI Bsp1286I BssSI BstXI BstYI
Bsu36I BtgI BtsI EarI EciI Eco57MI EcoNI EcoO109I
FspI Hin4I MslI MspA1I NcoI NsiI PmlI PspOMI
PstI PvuII SapI SfcI SmlI StuI StyI TaqII
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI
BciVI BclI BmgBI BmrI BmtI BplI BsaI BsaBI
BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EcoICRI EcoRI EcoRV FalI FseI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI
ZraI