(Linear) (Six-base) MAPSORT of: KIBB7515.seq  Check: 9353  from: 1  to: 381



KIBB7515.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     304     381 
   Size:        283      21      77

  Fragments arranged by size: 

                 283      77      21
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      49     381 
   Size:         49     332
 
AvaI C'yCGr_G

Cuts at:      0      55     381 
   Size:         55     326
 
BanI G'GyrC_C

Cuts at:      0      51     381 
   Size:         51     330
 
BanII G_rGCy'C

Cuts at:      0      14      94     381 
   Size:         14      80     287

  Fragments arranged by size: 

                 287      80      14
 
BbeI G_GCGC'C

Cuts at:      0      55     381 
   Size:         55     326
 
BglI GCCn_nnn'nGGC

Cuts at:      0     348     381 
   Size:        348      33
 
Bme1580I G_kGCm'C

Cuts at:      0     307     381 
   Size:        307      74
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     381 
   Size:        229     152
 
BsaHI Gr'CG_yC

Cuts at:      0      52     381 
   Size:         52     329
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      54     271     381 
   Size:         54     217     110

  Fragments arranged by size: 

                 217     110      54
 
BsiEI CG_ry'CG

Cuts at:      0      20     352     381 
   Size:         20     332      29

  Fragments arranged by size: 

                 332      29      20
 
BsiHKAI G_wGCw'C

Cuts at:      0     293     381 
   Size:        293      88
 
Bsp1286I G_dGCh'C

Cuts at:      0      14      94     293     307     381 
   Size:         14      80     199      14      74

  Fragments arranged by size: 

                 199      80      74      14      14
 
BsrFI r'CCGG_y

Cuts at:      0      22     364     381 
   Size:         22     342      17

  Fragments arranged by size: 

                 342      22      17
 
BssSI C'ACGA_G

Cuts at:      0     292     381 
   Size:        292      89
 
Bsu36I CC'TnA_GG

Cuts at:      0      95     381 
   Size:         95     286
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     112     381 
   Size:        112     269
 
EaeI y'GGCC_r

Cuts at:      0      17     349     362     381 
   Size:         17     332      13      19

  Fragments arranged by size: 

                 332      19      17      13
 
EagI C'GGCC_G

Cuts at:      0      17     349     381 
   Size:         17     332      32

  Fragments arranged by size: 

                 332      32      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     283     304     381 
   Size:        229      54      21      77

  Fragments arranged by size: 

                 229      77      54      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     274     381 
   Size:        274     107
 
HaeII r_GCGC'y

Cuts at:      0      55     381 
   Size:         55     326
 
KasI G'GCGC_C

Cuts at:      0      51     381 
   Size:         51     330
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     381 
   Size:        136     245
 
MspA1I CmG'CkG

Cuts at:      0     309     381 
   Size:        309      72
 
NaeI GCC'GGC

Cuts at:      0      24     366     381 
   Size:         24     342      15

  Fragments arranged by size: 

                 342      24      15
 
NarI GG'CG_CC

Cuts at:      0      52     381 
   Size:         52     329
 
NgoMIV G'CCGG_C

Cuts at:      0      22     364     381 
   Size:         22     342      17

  Fragments arranged by size: 

                 342      22      17
 
PstI C_TGCA'G

Cuts at:      0      38     381 
   Size:         38     343
 
SfcI C'TryA_G

Cuts at:      0      34     381 
   Size:         34     347
 
SfoI GGC'GCC

Cuts at:      0      53     381 
   Size:         53     328
 
SmaI CCC'GGG

Cuts at:      0      57     381 
   Size:         57     324
 
XmaI C'CCGG_G

Cuts at:      0      55     381 
   Size:         55     326
 

 Enzymes that do cut:

     AcuI     AlfI     AvaI     BanI    BanII     BbeI     BglI Bme1580I
     BpmI    BsaHI    BseRI    BsiEI  BsiHKAI Bsp1286I    BsrFI    BssSI
   Bsu36I     DrdI     EaeI     EagI  Eco57MI    EcoNI    HaeII     KasI
     MmeI   MspA1I     NaeI     NarI   NgoMIV     PstI     SfcI     SfoI
     SmaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseYI     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     MslI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI