(Linear) (Six-base) MAPSORT of: KIBB7516.seq  Check: 9291  from: 1  to: 738



KIBB7516.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0      81     738 
   Size:         81     657
 
AclI AA'CG_TT

Cuts at:      0     488     738 
   Size:        488     250
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81     565     738 
   Size:         81     484     173

  Fragments arranged by size: 

                 484     173      81
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      78     738 
   Size:         78     660
 
ApaLI G'TGCA_C

Cuts at:      0      63     738 
   Size:         63     675
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     553     738 
   Size:        553     185
 
BbvCI CC'TCA_GC

Cuts at:      0     728     738 
   Size:        728      10
 
Bme1580I G_kGCm'C

Cuts at:      0      39      67     738 
   Size:         39      28     671

  Fragments arranged by size: 

                 671      39      28
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     738 
   Size:         71     667
 
Bpu10I CC'TnA_GC

Cuts at:      0     518     728     738 
   Size:        518     210      10

  Fragments arranged by size: 

                 518     210      10
 
BseYI C'CCAG_C

Cuts at:      0     254     738 
   Size:        254     484
 
BsiHKAI G_wGCw'C

Cuts at:      0      67     738 
   Size:         67     671
 
Bsp1286I G_dGCh'C

Cuts at:      0      39      67     738 
   Size:         39      28     671

  Fragments arranged by size: 

                 671      39      28
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     312     738 
   Size:        312     426
 
BsrFI r'CCGG_y

Cuts at:      0      67     738 
   Size:         67     671
 
BstYI r'GATC_y

Cuts at:      0     684     738 
   Size:        684      54
 
BtsI GCAGTG_nn'

Cuts at:      0     448     738 
   Size:        448     290
 
DraI TTT'AAA

Cuts at:      0     204     351     413     738 
   Size:        204     147      62     325

  Fragments arranged by size: 

                 325     204     147      62
 
EarI CTCTTCn'nnn_

Cuts at:      0     329     738 
   Size:        329     409
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     328     738 
   Size:        328     410
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71      81     565     738 
   Size:         71      10     484     173

  Fragments arranged by size: 

                 484     173      71      10
 
HincII GTy'rAC

Cuts at:      0      82     446     738 
   Size:         82     364     292

  Fragments arranged by size: 

                 364     292      82
 
HpaI GTT'AAC

Cuts at:      0     446     738 
   Size:        446     292
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     654     738 
   Size:        241     413      84

  Fragments arranged by size: 

                 413     241      84
 
NspI r_CATG'y

Cuts at:      0      21     610     738 
   Size:         21     589     128

  Fragments arranged by size: 

                 589     128      21
 
PasI CC'CwG_GG

Cuts at:      0      39     738 
   Size:         39     699
 
PsiI TTA'TAA

Cuts at:      0     485     738 
   Size:        485     253
 
SalI G'TCGA_C

Cuts at:      0      80     738 
   Size:         80     658
 
SfcI C'TryA_G

Cuts at:      0     230     738 
   Size:        230     508
 
SphI G_CATG'C

Cuts at:      0     610     738 
   Size:        610     128
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AlfI    ApaLI     BbsI    BbvCI Bme1580I
     BpmI   Bpu10I    BseYI  BsiHKAI Bsp1286I    BspMI    BsrFI    BstYI
     BtsI     DraI     EarI     EciI  Eco57MI   HincII     HpaI     MmeI
     NspI     PasI     PsiI     SalI     SfcI     SphI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI