(Linear) (Six-base) MAPSORT of: KIBB7518.seq  Check: 878  from: 1  to: 738



KIBB7518.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     718     738 
   Size:        718      20
 
AflIII A'CryG_T

Cuts at:      0     130     525     738 
   Size:        130     395     213

  Fragments arranged by size: 

                 395     213     130
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     281     738 
   Size:        281     457
 
AlwNI CAG_nnn'CTG

Cuts at:      0     649     738 
   Size:        649      89
 
ApoI r'AATT_y

Cuts at:      0      35     559     571     738 
   Size:         35     524      12     167

  Fragments arranged by size: 

                 524     167      35      12
 
BamHI G'GATC_C

Cuts at:      0      59     738 
   Size:         59     679
 
BanI G'GyrC_C

Cuts at:      0     321     738 
   Size:        321     417
 
BanII G_rGCy'C

Cuts at:      0     227     738 
   Size:        227     511
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     611     738 
   Size:        326     285     127

  Fragments arranged by size: 

                 326     285     127
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     738 
   Size:        203     535
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     664     738 
   Size:        664      74
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     676     738 
   Size:        676      62
 
BseYI C'CCAG_C

Cuts at:      0      30     738 
   Size:         30     708
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     738 
   Size:        598     140
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     738 
   Size:        227     511
 
BspHI T'CATG_A

Cuts at:      0     453     738 
   Size:        453     285
 
BsrDI GCAATG_nn'

Cuts at:      0     299     738 
   Size:        299     439
 
BsrGI T'GTAC_A

Cuts at:      0     684     738 
   Size:        684      54
 
BstYI r'GATC_y

Cuts at:      0      59     738 
   Size:         59     679
 
Bsu36I CC'TnA_GG

Cuts at:      0     659     738 
   Size:        659      79
 
BtsI GCAGTG_nn'

Cuts at:      0     371     738 
   Size:        371     367
 
EarI CTCTTCn'nnn_

Cuts at:      0     452     738 
   Size:        452     286
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     611     718     738 
   Size:        326     285     107      20

  Fragments arranged by size: 

                 326     285     107      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     425     738 
   Size:        425     313
 
EcoO109I rG'GnC_Cy

Cuts at:      0     709     738 
   Size:        709      29
 
EcoRV GAT'ATC

Cuts at:      0      15     415     738 
   Size:         15     400     323

  Fragments arranged by size: 

                 400     323      15
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     131     738 
   Size:        131     607
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     319     351     738 
   Size:        319      32     387

  Fragments arranged by size: 

                 387     319      32
 
HincII GTy'rAC

Cuts at:      0      48     738 
   Size:         48     690
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     738 
   Size:        171     567
 
MspA1I CmG'CkG

Cuts at:      0     255     738 
   Size:        255     483
 
NspI r_CATG'y

Cuts at:      0     134     217     738 
   Size:        134      83     521

  Fragments arranged by size: 

                 521     134      83
 
PciI A'CATG_T

Cuts at:      0     130     738 
   Size:        130     608
 
PfoI T'CCnGG_A

Cuts at:      0      86     738 
   Size:         86     652
 
PsiI TTA'TAA

Cuts at:      0     343     738 
   Size:        343     395
 
PstI C_TGCA'G

Cuts at:      0     366     738 
   Size:        366     372
 
SapI GCTCTTCn'nnn_

Cuts at:      0     452     738 
   Size:        452     286
 
SfcI C'TryA_G

Cuts at:      0     362     738 
   Size:        362     376
 
SmlI C'TyrA_G

Cuts at:      0     218     738 
   Size:        218     520
 
SphI G_CATG'C

Cuts at:      0     217     738 
   Size:        217     521
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     582     738 
   Size:        582     156
 
TatI w'GTAC_w

Cuts at:      0     200     684     738 
   Size:        200     484      54

  Fragments arranged by size: 

                 484     200      54
 
XbaI T'CTAG_A

Cuts at:      0     349     738 
   Size:        349     389
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI    AlwNI     ApoI    BamHI     BanI    BanII
     BpmI    BpuEI     BsaI    BseRI    BseYI     BsgI Bsp1286I    BspHI
    BsrDI    BsrGI    BstYI   Bsu36I     BtsI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI    Hin4I   HincII     MmeI   MspA1I     NspI
     PciI     PfoI     PsiI     PstI     SapI     SfcI     SmlI     SphI
    TaqII     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoRI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI