(Linear) (Six-base) MAPSORT of: KIBB7519.seq  Check: 2091  from: 1  to: 426



KIBB7519.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     319     426 
   Size:        319     107
 
ApoI r'AATT_y

Cuts at:      0      99     426 
   Size:         99     327
 
BanII G_rGCy'C

Cuts at:      0     170     426 
   Size:        170     256
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     171     426 
   Size:        171     255
 
BbvCI CC'TCA_GC

Cuts at:      0     323     426 
   Size:        323     103
 
BglI GCCn_nnn'nGGC

Cuts at:      0     298     426 
   Size:        298     128
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      66     426 
   Size:         66     360
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     426 
   Size:        247     179
 
Bpu10I CC'TnA_GC

Cuts at:      0     323     426 
   Size:        323     103
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     226     426 
   Size:        226     200
 
BsaHI Gr'CG_yC

Cuts at:      0      47     426 
   Size:         47     379
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     346     376     426 
   Size:        346      30      50

  Fragments arranged by size: 

                 346      50      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55     340     388     426 
   Size:         55     285      48      38

  Fragments arranged by size: 

                 285      55      48      38
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     426 
   Size:        170     256
 
BsiHKAI G_wGCw'C

Cuts at:      0     170     426 
   Size:        170     256
 
Bsp1286I G_dGCh'C

Cuts at:      0     170     426 
   Size:        170     256
 
BspHI T'CATG_A

Cuts at:      0     182     426 
   Size:        182     244
 
BsrDI GCAATG_nn'

Cuts at:      0     236     426 
   Size:        236     190
 
BtgI C'CryG_G

Cuts at:      0      22     426 
   Size:         22     404
 
EaeI y'GGCC_r

Cuts at:      0     299     426 
   Size:        299     127
 
EcoICRI GAG'CTC

Cuts at:      0     168     426 
   Size:        168     258
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     426 
   Size:        247     179
 
EcoRV GAT'ATC

Cuts at:      0     399     426 
   Size:        399      27
 
HindIII A'AGCT_T

Cuts at:      0     271     348     426 
   Size:        271      77      78

  Fragments arranged by size: 

                 271      78      77
 
MscI TGG'CCA

Cuts at:      0     301     426 
   Size:        301     125
 
MspA1I CmG'CkG

Cuts at:      0      24      85     426 
   Size:         24      61     341

  Fragments arranged by size: 

                 341      61      24
 
NdeI CA'TA_TG

Cuts at:      0     113     426 
   Size:        113     313
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     152     184     426 
   Size:        152      32     242

  Fragments arranged by size: 

                 242     152      32
 
PstI C_TGCA'G

Cuts at:      0     383     426 
   Size:        383      43
 
PvuII CAG'CTG

Cuts at:      0      85     426 
   Size:         85     341
 
SacI G_AGCT'C

Cuts at:      0     170     426 
   Size:        170     256
 
SacII CC_GC'GG

Cuts at:      0      25     426 
   Size:         25     401
 
SfcI C'TryA_G

Cuts at:      0     379     426 
   Size:        379      47
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     298     426 
   Size:        298     128
 
StyI C'CwwG_G

Cuts at:      0     332     426 
   Size:        332      94
 

 Enzymes that do cut:

    AlwNI     ApoI    BanII     BbsI    BbvCI     BglI     BplI     BpmI
   Bpu10I     BsaI    BsaHI    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I
    BspHI    BsrDI     BtgI     EaeI  EcoICRI  Eco57MI    EcoRV  HindIII
     MscI   MspA1I     NdeI     PpiI     PstI    PvuII     SacI    SacII
     SfcI     SfiI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI    BpuEI    BsaAI    BsaBI    BsaWI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI